FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004686845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300319
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATAGTGACTCACCTCGGCTCAGCTTCGGCGGAGGGACCAAAGTGGAGATCAAACGAA54730.4208967184206337No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54680.42051219739156315No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46810.3599885874158572No Hit
GTATAGTGACTCACCTCGGCTCAGCTTCGGCGGAGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCAT39640.30484827184713903No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCACTCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA34000.26147429976798003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32860.25270722030517123No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32560.25040009413074793No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31450.2418637272853815No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30500.23455782773304087No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30350.2334042646458292No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28170.21663914777835286No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27740.21333226692834603No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCTGCAGGGCCAGTCAGAATGTGGGCAC27110.208487301962057No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25930.1994126056759918No Hit
GTAATACACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGAAGATACA25890.19910498885273537No Hit
CATCTATGGTGCATCTAAAAGGGCCACTGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA25840.19872046782366481No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGAGTGGAGCCTGAGGATT25370.19510597015040154No Hit
GTGTATTACTGTCAGCAGTATAGTGACTCACCTCGGCTCAGCTTCGGCGG23440.18026345842827798No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23330.17941751216432275No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23110.17772561963641229No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22820.17549539766780306No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22460.17272684625849502No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22190.17065043270151403No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21940.16872782755616123No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCAGTGGCCCTTTT20750.15957622706428193No Hit
GTGTATTACTGTGCGAGGGACCTCCGCCTATGGTTCGCGGAATTGGGGGT20270.15588482518520455No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCTGC19990.1537315074224094No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19840.15257794433519775No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19740.15180890227705662No Hit
CTCCTGCAGGGCCAGTCAGAATGTGGGCACCAGCTACTTAGTCTGGTACC19330.14865582983867806No Hit
GCAGTATAGTGACTCACCTCGGCTCAGCTTCGGCGGAGGGACCAAAGTGG19290.1483482130154216No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCTCCGCCGAAGCTGAGCCGAGGTGAGTCACTATACTG18980.14596418263518413No Hit
CCATAGATGAGGACCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGACTAAGTAGCTGGTGCCCACATTCTGA18740.14411848169564545No Hit
GTCAGAATGTGGGCACCAGCTACTTAGTCTGGTACCAGCAGAAACCTGGC18720.14396467328401724No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18160.13965803775842697No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17980.13827376205377298No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17910.13773543261307417No Hit
GTCACTATACTGCTGACAGTAATACACTGCAAAATCCTCAGGCTCCACTCTGCTGATGGAGAGAGTGAAGT17690.13604354008516373No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17630.13558211485027905No Hit
CTCCATCAGCAGAGTGGAGCCTGAGGATTTTGCAGTGTATTACTGTCAGCAGTATAGTGACTCACCTCGGCTCAG17430.13404403073399682No Hit
GTAATACACTGCAAAATCCTCAGGCTCCACTCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17180.132121425588644No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17080.1313523835305029No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16990.13066024567817588No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16840.12950668259096423No Hit
GACTTCACTCTCTCCATCAGCAGAGTGGAGCCTGAGGATTTTGCAGTGTATTACTGTCAGCAGTATAGTGACTCA15930.12250839986188003No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15640.1202781778932708No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCTGCAGGGCCAGTCAGA15530.11943223162931557No Hit
CTATACTGCTGACAGTAATACACTGCAAAATCCTCAGGCTCCACTCTGCT15450.11881699798280268No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15070.11589463816186643No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14990.11527940451535354No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14980.11520250030953942No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14860.11427964983977008No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14750.11343370357581486No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCC14540.1118187152537185No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14440.11104967319557739No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13950.10728136711068592No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13800.10612780402347423No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT13740.10566637878858956No Hit
GCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGT13720.10551257037696134No Hit
CCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCTCC13510.103897582054865No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13500.10382067784905089No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13460.10351306102579444No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13440.10335925261416622No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13370.10282092317346744No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13360.10274401896765332No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13300.10228259373276866No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13260.10197497690951221No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13150.10112903064555696No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG13060.10043689279322997No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCA13020.10012927596997352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)