FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300319
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51970.39967115761593885No Hit
GTATTACTGTCAGCAGTATAGTGACTCACCTCGGCTCAGCTTCGGCGGAGGGACCAAAGTGGAGATCAAACGAA51770.3981330734996566No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38720.2977730849122408No Hit
GTATAGTGACTCACCTCGGCTCAGCTTCGGCGGAGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCAT37640.2894674306843167No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34710.266934498380782No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33950.26108977873890943No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31060.23886446325863114No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCACTCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA31010.2384799422295606No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29900.2299435753841942No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28140.21640843516091052No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGAGTGGAGCCTGAGGATT25430.19556739538528622No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25180.19364479023993342No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25130.19326026921086287No Hit
CATCTATGGTGCATCTAAAAGGGCCACTGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA25110.19310646079923466No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24330.18710793274573392No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCTGCAGGGCCAGTCAGAATGTGGGCAC24300.1868772201282916No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24150.18572365704107993No Hit
GTAATACACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGAAGATACA23680.18210915936781666No Hit
GTGTATTACTGTCAGCAGTATAGTGACTCACCTCGGCTCAGCTTCGGCGG23020.1770334817840853No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22580.17364969672826436No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22470.17280375046430915No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21470.16511332988289798No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20380.15673077144915978No Hit
GTGTATTACTGTGCGAGGGACCTCCGCCTATGGTTCGCGGAATTGGGGGT19580.15057843498403084No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCAGTGGCCCTTTT19500.14996320133751795No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCTGC19300.1484251172212357No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19230.14788678778053693No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19110.1469639373107676No Hit
GCAGTATAGTGACTCACCTCGGCTCAGCTTCGGCGGAGGGACCAAAGTGG19060.14657941628169702No Hit
CTCCTGCAGGGCCAGTCAGAATGTGGGCACCAGCTACTTAGTCTGGTACC19000.14611799104681236No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18830.14481061954797247No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCTCCGCCGAAGCTGAGCCGAGGTGAGTCACTATACTG18620.1431956312258761No Hit
GTCAGAATGTGGGCACCAGCTACTTAGTCTGGTACCAGCAGAAACCTGGC17960.13811995364214472No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17590.1352744980270226No Hit
GTCACTATACTGCTGACAGTAATACACTGCAAAATCCTCAGGCTCCACTCTGCTGATGGAGAGAGTGAAGT17540.13488997699795205No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17280.13289046764678514No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17170.1320445213828299No Hit
CTCCATCAGCAGAGTGGAGCCTGAGGATTTTGCAGTGTATTACTGTCAGCAGTATAGTGACTCACCTCGGCTCAG16970.13050643726654768No Hit
CCATAGATGAGGACCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGACTAAGTAGCTGGTGCCCACATTCTGA16860.12966049100259244No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16850.12958358679677834No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16660.12812240688631021No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16580.1275071732397973No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCTGCAGGGCCAGTCAGA16540.12719955641654085No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15930.12250839986188003No Hit
GTAATACACTGCAAAATCCTCAGGCTCCACTCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15760.12120102836304014No Hit
CTATACTGCTGACAGTAATACACTGCAAAATCCTCAGGCTCCACTCTGCT15480.11904771060024502No Hit
GACTTCACTCTCTCCATCAGCAGAGTGGAGCCTGAGGATTTTGCAGTGTATTACTGTCAGCAGTATAGTGACTCA15220.1170482012490781No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15090.11604844657349465No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14940.11489488348628298No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14730.11327989516418664No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14630.11251085310604553No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14550.11189561945953261No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14540.1118187152537185No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCA14410.11081896057813505No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14180.10905016384441049No Hit
CTCCTGTGCTGCCTCTGGGTTCACCGTCAGTAACAGTTACATGACGTGGGTCCGCCAGGCTCCAGGAAAGGGG14150.10881945122696815No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14150.10881945122696815No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13860.1065892292583589No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC13710.10543566617114723No Hit
GCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGT13610.10466662411300612No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13600.104589719907192No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCC13580.10443591149556378No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13510.103897582054865No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13380.10289782737928155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)