FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569603
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT79400.5058603990945482No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71080.4528533648317441No Hit
GAGTTACAGTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT69100.4402387100432403No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGCTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTC59520.37920416818775193No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56660.3609830001599131No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51450.3277898933679408No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG45890.2923669233557785No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45880.2923032129780588No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45180.2878434865376786No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44640.2844031261408139No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44530.28370231198589707No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGTAAATTAAATTGGTATCAGAAAAAG44160.28134502801026756No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG40670.25911010618608654No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT39040.2487253146177728No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38400.24464785044371096No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC38100.24273653911211943No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG37010.23579210794067038No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT36220.23075898810081277No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC35120.22375084655164396No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34240.2181443333123089No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34050.2169338361356343No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33650.21438542102684566No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG33370.2126015304506936No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32850.20928859080926832No Hit
GACTTCACTCTCACCATCAGCAGTCTGCAAGCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCC32740.20858777665435144No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32130.20470144361344877No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31980.20374578794765302No Hit
GAGTGAAGTCTGTCCCAGATCCAGTGCCACTGAACCTTGATGGGACCCCA29740.18947466333843652No Hit
ACAGTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA29300.186671406718769No Hit
GCCCCTAAGCTCCTAATCTACGCTGCTTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCACTGGAT28500.1815745765011917No Hit
GTCCCAGATCCAGTGCCACTGAACCTTGATGGGACCCCAGTTTGCAAACT28490.181510866123472No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGCA28310.1803640793245171No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27750.17679629817221296No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT27740.17673258779449327No Hit
ACTTACTACTGTCAACAGAGTTACAGTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA27030.1722091509763934No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26850.1710623641774385No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAAGCTGAAGATT26820.17087123304427934No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26180.1667937688702175No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25960.16539214056038373No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25850.16469132640546685No Hit
GCTTAGGGGCTGTCCCTGGCTTTTTCTGATACCAATTTAATTTACTGCTA25380.16169693865264018No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24260.15456137634803194No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24180.15405169332627422No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24140.15379685181539535No Hit
AATCTACGCTGCTTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCACTGGATCTGGGACAGACTTCA23840.15188554048380387No Hit
GATTAGGAGCTTAGGGGCTGTCCCTGGCTTTTTCTGATACCAATTTAATT23620.1504839121739701No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGAAGGGACA23410.14914599424185607No Hit
GTAGTAAGTTGCAAAATCTTCAGCTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCAGTGCCA23390.14901857348641662No Hit
GGGCACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGCTTGCAGACTGCTGATGGTGAGAGTGAAGT22990.14647015837762797No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22840.14551450271183222No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22300.14207414231496754No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT22120.14092735551601263No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22060.14054509324969436No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA21340.13595794605387476No Hit
GCGTAGATTAGGAGCTTAGGGGCTGTCCCTGGCTTTTTCTGATACCAATT21200.13506600076579875No Hit
CACCATCAGCAGTCTGCAAGCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCCCCGCTCACTTT20980.133664372455965No Hit
GTGTATTACTGTGCGAGAGCAATTGCCACCTACCGGGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGT20930.13334582056736638No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20920.1332821101896467No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20870.1329635583010481No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20730.13207161301297207No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20690.1318167715020932No Hit
CTCCTAATCTACGCTGCTTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCACTGGATCTGGGACAGA20600.13124337810261577No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20580.13111595734717632No Hit
GTCTCCAACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT20420.13009659130366086No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20330.1295231979041834No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20220.12882238374926652No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20050.12773930732803135No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19850.12646509977363704No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19500.12423523655344697No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19450.12391668466484836No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19370.12340700164309064No Hit
ACCTTGATGGGACCCCAGTTTGCAAACTGGAAGCAGCGTAGATTAGGAGCTTAGGGGCTGTCCCTGGCTTTTT19120.12181424220009773No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18950.12073116577886256No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18950.12073116577886256No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18870.12022148275710481No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18570.11831017142551334No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18270.11639886009392185No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT18110.11537949405040639No Hit
CATCAAGGTTCAGTGGCACTGGATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAAGCTGAAGATTTTG18080.11518836291724721No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17740.11302221007477688No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17730.11295849969705715No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17610.11219397516442056No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17590.11206655440898113No Hit
GCCAGGGACAGCCCCTAAGCTCCTAATCTACGCTGCTTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGG17450.1111746091209051No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC16880.10754311759088127No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16800.10703343456912352No Hit
AGTCAGAGCATTAGCAGTAAATTAAATTGGTATCAGAAAAAGCCAGGGACAGCCCCTAAGCTCCTAATCTACG16790.10696972419140381No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16680.10626891003648693No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16620.10588664777016862No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16620.10588664777016862No Hit
CCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCG16530.10531325437069118No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16530.10531325437069118No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16500.10512212323753203No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGCACTGTAACTCTGTTGACA16460.10486728172665316No Hit
GTCCCTGGCTTTTTCTGATACCAATTTAATTTACTGCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACT16180.10308339115050111No Hit
GGATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAAGCTGAAGA16160.10295597039506167No Hit
GCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCAGTGCCACTGAACCTTG16050.10225515624014479No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15860.10104465906347018No Hit
CTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCAGTGCCACTGAACCT15760.10040755528627303No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCA15720.10015271377539416No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15710.10008900339767446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)