FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569603
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81470.5190484472825294No Hit
GAGTTACAGTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT65970.42029736181696903No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63670.4056439749414342No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGCTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTC56860.36225720771430736No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56140.35767006051848776No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54700.3484957661268486No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45810.2918572403340208No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGTAAATTAAATTGGTATCAGAAAAAG45730.29134755731226303No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45310.28867172144803493No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG42940.2735723619284622No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA42240.269112635488082No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41450.2640795156482244No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC39840.2538221448353501No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37350.2379582607831407No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT35950.2290388079023804No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35400.225534737127796No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35070.22343229466304537No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG34670.2208838795542567No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT33370.2126015304506936No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33190.2114547436517387No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC32590.2076321209885557No Hit
GACTTCACTCTCACCATCAGCAGTCTGCAAGCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCC32570.20750470023311626No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31910.203299815303615No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31100.198139274708318No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30630.1951448869554913No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG30320.1931698652461801No Hit
GAGTGAAGTCTGTCCCAGATCCAGTGCCACTGAACCTTGATGGGACCCCA29540.1882004557840422No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29240.18628914445245073No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29070.18520606803121553No Hit
GCCCCTAAGCTCCTAATCTACGCTGCTTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCACTGGAT28900.18412299160998036No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28620.18233910103382828No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27410.1746301453297426No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT27150.17297367550902998No Hit
GTCCCAGATCCAGTGCCACTGAACCTTGATGGGACCCCAGTTTGCAAACT26800.17074381228883992No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26660.16985186700076388No Hit
AATCTACGCTGCTTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCACTGGATCTGGGACAGACTTCA26610.1695333151121653No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26400.16819539718005125No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26370.16800426604689211No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGCA26110.16634779622617948No Hit
ACAGTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA25850.16469132640546685No Hit
ACTTACTACTGTCAACAGAGTTACAGTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA25300.16118725563088246No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAAGCTGAAGATT25260.1609324141200036No Hit
GCTTAGGGGCTGTCCCTGGCTTTTTCTGATACCAATTTAATTTACTGCTA24820.15812915750033607No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24510.15615413579102486No Hit
GATTAGGAGCTTAGGGGCTGTCCCTGGCTTTTTCTGATACCAATTTAATT24330.15500734899206997No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23760.15137585746204613No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23480.14959196688589407No Hit
GTAGTAAGTTGCAAAATCTTCAGCTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCAGTGCCA23090.14710726215482514No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA22610.14404916402427875No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22410.14277495646988442No Hit
CTCCTAATCTACGCTGCTTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCACTGGATCTGGGACAGA21830.1390797545621409No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21570.13742328474142823No Hit
GGGCACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGCTTGCAGACTGCTGATGGTGAGAGTGAAGT21480.1368498913419508No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21470.1367861809642311No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGAAGGGACA21410.13640391869791277No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21020.13391921396684384No Hit
GTGTATTACTGTGCGAGAGCAATTGCCACCTACCGGGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGT20680.1317530611243735No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT20560.1309885365917369No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20540.13086111583629745No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20530.13079740545857774No Hit
GTCTCCAACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT20040.12767559695031164No Hit
CACCATCAGCAGTCTGCAAGCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCCCCGCTCACTTT19910.12684736203995534No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19790.12608283750731872No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19650.1251908922192427No Hit
GCGTAGATTAGGAGCTTAGGGGCTGTCCCTGGCTTTTTCTGATACCAATT19610.12493605070836382No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19480.12410781579800753No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19370.12340700164309064No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19090.12162311106693859No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19060.12143197993377942No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18830.11996664124622596No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18760.11952066860218793No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18660.11888356482499078No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18490.1178004884037556No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18210.11601659782760354No Hit
ACCTTGATGGGACCCCAGTTTGCAAACTGGAAGCAGCGTAGATTAGGAGCTTAGGGGCTGTCCCTGGCTTTTT18090.11525207329496695No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17330.11041008458826851No Hit
CATCAAGGTTCAGTGGCACTGGATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAAGCTGAAGATTTTG17250.10990040156651076No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17190.10951813930019245No Hit
GTCCCTGGCTTTTTCTGATACCAATTTAATTTACTGCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACT17040.10856248363439673No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16980.10818022136807842No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16920.10779795910176014No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16900.1076705383463207No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16810.10709714494684325No Hit
AGTCAGAGCATTAGCAGTAAATTAAATTGGTATCAGAAAAAGCCAGGGACAGCCCCTAAGCTCCTAATCTACG16690.10633262041420666No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16670.10620519965876722No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16580.10563180625928978No Hit
GCCAGGGACAGCCCCTAAGCTCCTAATCTACGCTGCTTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGG16480.1049947024820926No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC16370.10429388832717572No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16270.10365678454997856No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15950.10161805246294764No Hit
CTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCAGTGCCACTGAACCT15810.10072610717487161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)