FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022239
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25610.2505284967605423No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21070.2061161822235309No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18010.17618189092765976No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC17770.17383410337504243No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17530.17148631582242507No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16210.1585734842830297No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14650.143312865191017No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14110.13803034319762794No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13480.13186740087200743No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13200.12912831539395386No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13140.1285413685057995No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13110.12824789506172235No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12830.12550880958366878No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12560.12286754858697427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12510.122378426180179No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12060.11797632451902149No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11860.11601983489184035No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG11720.11465029215281357No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11650.11396552078330019No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC11560.11308510045106868No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11520.11269380252563246No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11470.11220468011883718No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG11440.11191120667476001No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11030.10790040293903871No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCGCCGCCGAATACCCA10880.10643303571865288No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC10320.10095486476254575No Hit
GACCTAGGACGGTCAGCCTGGTCCCGCCGCCGAATACCCAACCACTGCTGTCTGCTGATTGACAGTAATAGTCAG10310.10085704028118668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13650.045.4028471
GTGGTAT4450.039.573821
TGGTATC4650.037.1343352
GTATCAA35300.035.4971
GTCGGTT1201.0266376E-832.167369
AATCGAT553.6028135E-430.8280522
ATCAACG40750.030.1589473
TCAACGC41150.029.7835144
TCGGTTT2201.8681021E-929.73402470
CAACGCA42200.029.2071955
CGGGTAT704.3437114E-529.05363717
AACGCAG43150.028.489876
TCGATAA605.9862674E-428.26735724
TATCAAC44000.028.2404632
TATATCG606.048311E-428.2175715
GTAACCG500.00815436127.30257832
CGCAGAG45100.027.1251188
ACTGTCG500.008440725527.1100888
AGAGTAC45700.026.9291611
GCAGAGT46250.026.5291219
Produced by FastQC (version 0.11.5)