FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255352
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGA54120.43111414169093615No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43040.3428520446854747No Hit
CAGTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGACCCTCTGCCA41340.32931002619185695No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40580.3232559473358867No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCA32010.25498824234159023No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30840.24566814726068864No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29540.23531248605968683No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27880.22208910329533071No Hit
GTCCCAGACCCTCTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT26410.21037924024496715No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26170.2084674258693976No Hit
GTCTGGGACAGACTTCACTCTCATCGTCAGTCGCCTGGAGCCTGAAGACT24890.19827108253302658No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24810.1976338110745034No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24060.19165939115084854No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23900.19038484823380217No Hit
GTCAGAGTGTGAGCGGTTACTTGGCCTGGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATG22870.18217997820531612No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21490.17118704554579114No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT21110.16816000611780602No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21080.1679210293208598No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20950.16688546320075962No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20080.15995513608931997No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20080.15995513608931997No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20040.1596365003600584No Hit
CTCCTGCAGGGCCAGTCAGAGTGTGAGCGGTTACTTGGCCTGGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACT19820.1578840038491196No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCCCGGCCAAAAGTC19130.1523875375193571No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAGGAGCCACCCTCTCCT19040.15167060712851851No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18880.15039606421147217No Hit
CTCCCAGACTCCTCATCTATGATGCATCCACCAGGGCCACTGGCATCCCA18640.1484842498359026No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18610.1482452730389564No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAGGAGCCACCCTCTCCTGC17610.14027937980741656No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17440.13892517795805479No Hit
ATGTATTACTGTCAGCAATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGACCAAAGTGGAGATCAAACGAA16960.1351015492069157No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16380.13048133113262256No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTAC15830.12610008985527565No Hit
GTCTTTGTCTCCAGGTGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15510.12355100402118291No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15390.12259509683339813No Hit
GTCTGGGAGCCTGGCCAGGCTTCTGCTGATACCAGGCCAAGTAACCGCTC15110.12036464672856696No Hit
GTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGACCCTCTGCCA15090.12020532886393617No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14780.11773590196215881No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14670.11685965370668945No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14490.11542579292501227No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14480.11534613399269687No Hit
GAGTGAAGTCTGTCCCAGACCCTCTGCCACTGAACCTGTCTGGGATGCCA14320.1140715910756505No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14320.1140715910756505No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCC14210.11319534282018111No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14040.11184114097081933No Hit
GTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGACC14000.11152250524155774No Hit
GACTTCACTCTCATCGTCAGTCGCCTGGAGCCTGAAGACTTTGCAATGTATTACTGTCAGCAATATGGTGCCTCA13910.11080557485071917No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13840.11024796232451137No Hit
GAGTGTGAGCGGTTACTTGGCCTGGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATC13780.10977000873061898No Hit
CACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCACCTGGAGACAAA13710.10921239620441119No Hit
GCAATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCAT13700.10913273727209578No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12820.10212275122834073No Hit
CTGCTACTCTGGCTCCCAGGTACCACCGGAGAAATTGTGTTGACGCAGTC12760.10164479763444835No Hit
CCATATTGCTGACAGTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACT12650.10076854937897896No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12570.10013127792045577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)