FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255352
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGA52070.4147840605662794No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44740.3563940631790924No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38540.3070055251435454No Hit
CAGTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGACCCTCTGCCA37240.2966498639425436No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36170.288126358184796No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31880.2539526762214901No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCA30780.24519019366679626No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30360.24184451850954952No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26000.20711322402003582No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25230.20097948623175013No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25090.19986426117933456No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25010.19922698972081138No Hit
GTCTGGGACAGACTTCACTCTCATCGTCAGTCGCCTGGAGCCTGAAGACT24180.1926152983386333No Hit
GTCCCAGACCCTCTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT23530.1874374677381324No Hit
GTCAGAGTGTGAGCGGTTACTTGGCCTGGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATG22110.17612589934934583No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20620.16425671843435147No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20430.16274319872035892No Hit
CTCCTGCAGGGCCAGTCAGAGTGTGAGCGGTTACTTGGCCTGGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACT19960.1589992289015352No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19690.15684843772901944No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19680.15676877879670403No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19550.15573321267660384No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAGGAGCCACCCTCTCCT19430.15477730548881907No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19320.15390105723334968No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19100.15214856072241092No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCCCGGCCAAAAGTC19050.15175026606083392No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19040.15167060712851851No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18140.1445013032201327No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAGGAGCCACCCTCTCCTGC17620.14035903873973196No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17440.13892517795805479No Hit
CTCCCAGACTCCTCATCTATGATGCATCCACCAGGGCCACTGGCATCCCA17440.13892517795805479No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17360.1382879064995316No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16480.13127792045577655No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT16390.13056099006493796No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15830.12610008985527565No Hit
GTCTTTGTCTCCAGGTGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15440.12299339149497511No Hit
ATGTATTACTGTCAGCAATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGACCAAAGTGGAGATCAAACGAA15300.12187816644255954No Hit
GTCTGGGAGCCTGGCCAGGCTTCTGCTGATACCAGGCCAAGTAACCGCTC14940.11901044487920519No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14940.11901044487920519No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTAC14860.118373173420682No Hit
GAGTGAAGTCTGTCCCAGACCCTCTGCCACTGAACCTGTCTGGGATGCCA14570.11606306438353546No Hit
GACTTCACTCTCATCGTCAGTCGCCTGGAGCCTGAAGACTTTGCAATGTATTACTGTCAGCAATATGGTGCCTCA14300.1139122732110197No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14280.1137529553463889No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14250.11351397854944272No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14250.11351397854944272No Hit
GTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGACCCTCTGCCA13980.11136318737692695No Hit
GAGTGTGAGCGGTTACTTGGCCTGGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATC13900.11072591591840376No Hit
GTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGACC13560.10801751221968023No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13430.10698194609958005No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13260.10562774425021827No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13150.10475149599474887No Hit
CTGCTACTCTGGCTCCCAGGTACCACCGGAGAAATTGTGTTGACGCAGTC13050.1039549066715949No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12830.10220241016065613No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCC12780.10180411549907914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)