FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336725
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC75770.5668331182554377No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCAAACACCCAAAAAATACCACTGCCCCAGGTCTGACAGTAATAGT75080.5616712487609643No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCAAACACCCAAAAAATACCACTGCCCCAGGTCTGA72090.5393031476182462No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG71590.5355626624773233No Hit
CTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT66750.4993547663131908No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53820.40262582056892776No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC52090.389683741981335No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49120.3674652602442537No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC45260.33858871495632986No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT43490.3253473975574632No Hit
GTCCAGCTCTGGGGCTCAGCGCTACCTCACCATCTCCAACCTCCAGTCTG42000.31420075183751334No Hit
GAGCTGGACCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGTTGTGGCT40090.2999120985991883No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT39890.2984159045428192No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39650.29662047167517624No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG39360.2944509902934411No Hit
GACTATTACTGTCAGACCTGGGGCAGTGGTATTTTTTGGGTGTTTGGCGGAGGGACCAGGCTGACCGTCCTAGGT39100.2925059380201612No Hit
CCTGCACTCTGAGCAGGGGGCACGGCAGCTACATCATCGCATGGCATCAG39040.2920570798032505No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG38810.290336456638426No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGC38800.2902616469356076No Hit
CACCTGCACTCTGAGCAGGGGGCACGGCAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCG38340.2868204006059586No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT37400.2797882885410238No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC37220.27844171389029154No Hit
CAACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGGTCCAGCTCTGGGGCTCAGCGCTACCTCACCATCTCCAA36800.2752997063719165No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC36750.27492565785782414No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCAAACACCCAAAAAATACCAC35850.2681927846041631No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC35050.26220800837868674No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC34460.25779423591239786No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA33980.254203370177112No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG33700.25210869849819517No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG32850.24574987375862647No Hit
GGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC30910.2312367914118461No Hit
CCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT30810.23048869438366154No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30060.2248779666722774No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29980.22427948904972977No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC29500.2206886233144439No Hit
GAGCAGGGGGCACGGCAGCTACATCATCGCATGGCATCAGCAGCAGCCAG28540.21350689184387212No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28230.21118779105650004No Hit
TCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGCGCTGAGCCCCAGAGCTGGACCCTGAGAAGCGATCAGGGATC27440.20527782453384205No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27390.20490377601974974No Hit
GCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG26520.1983953318745441No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGCGCTGAGCCCCAGAGCTGGACC26320.196899137818175No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25360.18971740634760328No Hit
GCTCTGGGGCTCAGCGCTACCTCACCATCTCCAACCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC24810.18560287269258824No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG24460.18298453309394228No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCAGTGGTATTT24410.18261048457985002No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24400.18253567487703154No Hit
CCCTTGTTGTGGCTGCCATCATTGTTGACTTTCATCAAGAAGCGAGGGGC24380.18238605547139464No Hit
CTATTACTGTCAGACCTGGGGCAGTGGTATTTTTTGGGTGTTTGGCGGAG24310.18186238755166545No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24160.18074024200938862No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT23950.17916923825020104No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23770.17782266359946886No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT23730.17752342478819502No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT23230.17378293964727226No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC23130.1730348426190877No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22760.17026688361480485No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCAAACAC22030.1648057753090576No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGGGGGCACGGC21770.16286072303577773No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC21710.162411864818867No Hit
GGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG21700.16233705511604857No Hit
GGGAAGGCCCCTCGCTTCTTGATGAAAGTCAACAATGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCG21270.15912023789485497No Hit
GCGCTACCTCACCATCTCCAACCTCCAGTCTGAGGATGAGGCTGACTATT21220.15874618938076268No Hit
GCCTGGTCCCTCCGCCAAACACCCAAAAAATACCACTGCCCCAGGTCTGA21080.1576988535413043No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCT20820.15575380126802446No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20480.15321027137219698No Hit
ACCTCACCATCTCCAACCTCCAGTCTGAGGATGAGGCTGACTATTACTGT19950.14924535712281883No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19860.14857206979745274No Hit
CCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCAGTG19520.14602853990162526No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG19520.14602853990162526No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC19520.14602853990162526No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18970.14191400624661019No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC18460.13809871140286895No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18420.13779947259159514No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGC18380.1375002337803213No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18300.13690175615777367No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA18170.13592923002113375No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18110.135480371804223No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG18100.13540556210140456No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT18000.13465746507322No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC17350.12979483439002037No Hit
GTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGGGGG17230.12889711795619893No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17140.1282238306308328No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16900.12642839776318987No Hit
GACTGGAGGTTGGAGATGGTGAGGTAGCGCTGAGCCCCAGAGCTGGACCCTGAGAAGCGATCAGGGATCCCGTCC16560.12388486786736239No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC16320.12208943499971946No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16230.12141614767435335No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC16030.11991995361798424No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC15860.11864818867007051No Hit
GCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCAGACTGGAGGTTGG15700.11745123342497522No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT15680.1173016140193383No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA15490.11588022966578765No Hit
CTTCTCAGGGTCCAGCTCTGGGGCTCAGCGCTACCTCACCATCTCCAACC15460.11565580055733228No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCAAACACC15450.11558099085451383No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC15410.11528175204324001No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG15200.11371074828405243No Hit
GACCTGGGGCAGTGGTATTTTTTGGGTGTTTGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGC15080.11281303185023098No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGC14960.1119153154164095No Hit
GCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGCGCTGAGCCCC14840.11101759898258805No Hit
GGATGAGGCTGACTATTACTGTCAGACCTGGGGCAGTGGTATTTTTTGGGTGTTTGGCGGAGGGACCAGGCTGAC14830.11094278927976958No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC14830.11094278927976958No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14790.11064355046849576No Hit
GGGCACGGCAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGC14690.10989545344031121No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCAAACACCCAAAAAATAC14650.10959621462903739No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14340.10727711384166526No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT14190.10615496829938843No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG14190.10615496829938843No Hit
CTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGCTTCTTGATGAAAGTCAACAATGATGGCAG14020.10488320335147469No Hit
TCTCAGGGTCCAGCTCTGGGGCTCAGCGCTACCTCACCATCTCCAACCTC13950.10435953543174549No Hit
GCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAG13940.10428472572892704No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG13880.10383586751201632No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC13830.10346181899792405No Hit
GCCCCAGAGCTGGACCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGTT13750.10286334137537638No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13740.10278853167255793No Hit
CTCCAACCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCAGTGGTATTTTTTGGGTGTTTGG13640.10204043464437336No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13440.10054424058800428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6400.049.16191
AGGGGGG1950.048.24622770
GTATCAA18000.041.989491
CTACGCT1550.041.97231328
TCGTATG856.548362E-1140.6146240
CGTATGC901.200533E-1038.3901541
TACGCTG1700.038.28464529
GGTACGT400.001606166138.04650568
ATCTCGT1300.037.07859837
TATCAAC20800.035.3505332
ACTACGC1850.035.15809227
CGAGACT1850.035.12389823
ACGAGAC1950.035.07649622
CACGAGA1950.035.07518421
CGACAGT400.002510085234.73886546
TCTCGTA1003.8562575E-1034.4625738
CCCACGA1900.034.20086319
ATCAACG22650.032.463183
TCAACGC23000.031.969184
ACGCTGC2050.031.75657330
Produced by FastQC (version 0.11.5)