FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336725
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC71990.5385550505900615No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCAAACACCCAAAAAATACCACTGCCCCAGGTCTGACAGTAATAGT68480.5122968449007836No Hit
CTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT66210.4953150423609942No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCAAACACCCAAAAAATACCACTGCCCCAGGTCTGA64480.4823729637734014No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG63220.472946941218276No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG57690.4315771755596701No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC49510.37038283865417343No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46480.3477154987001814No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC45600.3411322448521573No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG42780.3200359086573529No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT42060.31464961005442404No Hit
GTCCAGCTCTGGGGCTCAGCGCTACCTCACCATCTCCAACCTCCAGTCTG38820.2904112663412445No Hit
GGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC38470.2877929267425985No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT38000.2842768707101311No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT37920.28367839308758347No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG37700.28203257962557743No Hit
GAGCTGGACCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGTTGTGGCT36810.2753745160747349No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36560.27350427350427353No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC36150.27043707568871683No Hit
CCTGCACTCTGAGCAGGGGGCACGGCAGCTACATCATCGCATGGCATCAG36090.2699882174718061No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC35430.26505077708578806No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGC35220.26347977332660044No Hit
GACTATTACTGTCAGACCTGGGGCAGTGGTATTTTTTGGGTGTTTGGCGGAGGGACCAGGCTGACCGTCCTAGGT35120.26273167629841593No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34580.2586919523462193No Hit
CACCTGCACTCTGAGCAGGGGGCACGGCAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCG34440.25764461650676096No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC33700.25210869849819517No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA33310.24919112008827543No Hit
CAACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGGTCCAGCTCTGGGGCTCAGCGCTACCTCACCATCTCCAA33300.249116310385457No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG32310.2417101498064299No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31410.2349772765527689No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC30910.2312367914118461No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30380.227271877162468No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCAAACACCCAAAAAATACCAC30340.22697263835119416No Hit
CCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT30180.22577568310609888No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCAAACAC28320.21186107838186613No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26630.19921823860554713No Hit
GCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG26480.1980960930632703No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26090.19517851465335054No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26020.19465484673362135No Hit
GAGCAGGGGGCACGGCAGCTACATCATCGCATGGCATCAGCAGCAGCCAG25510.1908395518898801No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCAGTGGTATTT25190.18844564139968956No Hit
TCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGCGCTGAGCCCCAGAGCTGGACCCTGAGAAGCGATCAGGGATC25150.18814640258841572No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24760.18522882417849595No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24340.1820868166601208No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24180.18088986141502553No Hit
GCTCTGGGGCTCAGCGCTACCTCACCATCTCCAACCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC24140.1805906226037517No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGCGCTGAGCCCCAGAGCTGGACC23880.17864557033047188No Hit
CCCTTGTTGTGGCTGCCATCATTGTTGACTTTCATCAAGAAGCGAGGGGC23860.17849595092483495No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC23670.1770745665712843No Hit
CTATTACTGTCAGACCTGGGGCAGTGGTATTTTTTGGGTGTTTGGCGGAG23400.175054704595186No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT23370.17483027548673064No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT23190.17348370083599843No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22930.17153864856271858No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22650.16944397688380183No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC21820.16323477154987No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21380.15994314462585796No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21320.15949428640894722No Hit
GCGCTACCTCACCATCTCCAACCTCCAGTCTGAGGATGAGGCTGACTATT21090.15777366324412276No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC21060.1575492341356674No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20590.15403317810319997No Hit
GTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGGGGG20560.15380874899474462No Hit
GGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG20530.15358431988628926No Hit
GGGAAGGCCCCTCGCTTCTTGATGAAAGTCAACAATGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCG20360.1523125549383755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20260.15156445791019094No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC20020.14976902504254802No Hit
GCCTGGTCCCTCCGCCAAACACCCAAAAAATACCACTGCCCCAGGTCTGA19880.14872168920308965No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCT19670.14715068544390206No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCAAACACC19650.14700106603826515No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGC19550.1462529690100806No Hit
ACCTCACCATCTCCAACCTCCAGTCTGAGGATGAGGCTGACTATTACTGT19190.1435598197086162No Hit
CCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCAGTG19170.1434102003029793No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGGGGGCACGGC19110.14296134208606856No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18890.14131552862406252No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18790.140567431595878No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18630.1393704763507827No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17960.13435822626194618No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC17900.13390936804503545No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17790.13308646131403243No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA17690.13233836428584786No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT16850.12605434924909759No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16620.12433372608427312No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC16470.1232115805419963No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC16410.12276272232508556No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC16250.12156576707999028No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16010.11977033421234735No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC15920.11909704688698125No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15890.11887261777852587No Hit
GACTGGAGGTTGGAGATGGTGAGGTAGCGCTGAGCCCCAGAGCTGGACCCTGAGAAGCGATCAGGGATCCCGTCC15730.11767566253343058No Hit
CCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCT15580.11655351699115377No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCAAACACCCAAAAAATAC15540.11625427817987992No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15390.1151321326376031No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA15350.11483289382632927No Hit
CTTCTCAGGGTCCAGCTCTGGGGCTCAGCGCTACCTCACCATCTCCAACC15040.11251379303895716No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15040.11251379303895716No Hit
GGATGAGGCTGACTATTACTGTCAGACCTGGGGCAGTGGTATTTTTTGGGTGTTTGGCGGAGGGACCAGGCTGAC14960.1119153154164095No Hit
GCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCAGACTGGAGGTTGG14950.11184050571359104No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT14840.11101759898258805No Hit
GGGCACGGCAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGC14790.11064355046849576No Hit
GCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGCGCTGAGCCCC14410.10780078176139446No Hit
GACCTGGGGCAGTGGTATTTTTTGGGTGTTTGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGC14290.10690306532757299No Hit
CTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGCTTCTTGATGAAAGTCAACAATGATGGCAG14220.1063793974078438No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGC14200.10622977800220688No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC14140.10578091978529615No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT14120.10563130037965925No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT14060.10518244216274851No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13900.10398548691765322No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG13870.10376105780919787No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC13730.10271372196973949No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG13570.10151676672464419No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA13420.10039462118236736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7750.049.3436281
GACCGGA750.001965090436.51320370
TGGTATC1900.035.934362
GTATCAA18300.034.8903471
AATGGTA4450.033.84650870
ACGATTA450.00461161230.6820540
TATCAAC21250.029.559182
ATCAACG22800.027.5486453
TATTAGA1005.5038436E-727.3101122
TCAACGC23100.027.190874
CAACGCA23750.026.5914255
AACGCAG24300.026.132986
AACGGCC809.6833464E-525.82740437
CGCAGAG25200.025.072628
ACGCAGA25400.025.0049787
GAATACG1101.256718E-624.8441079
AGAGTAC25400.024.62009811
GTTCGTT700.001405349424.4233227
GTATTAG1401.5501428E-824.3885921
TAATTAG851.4300835E-424.38637541
Produced by FastQC (version 0.11.5)