FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277202
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA51160.40056310591433464No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGACC39920.312558232761928No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCACCGCCGAAGACCACGAGAGTGGTGCTGCCTGTATATGAA37190.29118338367775815No Hit
GACCTAGGACGGTCAGCTTGGTCCCACCGCCGAAGACCACGAGAGTGGTG36380.2848413954879494No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGACCTTCAAGCCAACAAGG34080.26683328087491254No Hit
GTATATTACTGTGCGAAAGAGACTATAGCAGATCGCCTTGGGGCTTATGA32350.2532880468398891No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG31490.24655457789762308No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG30610.23966451665437416No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT29810.23340082461505698No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29210.22870305558556908No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26960.21108642172498948No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26950.211008125574498No Hit
GTATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA25130.19675822618505137No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25080.19636674543259408No Hit
GAATAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT24740.19370467631588426No Hit
GGACTGAATAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT23660.18524869206280603No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23480.18383936135395967No Hit
GTTCATATACAGGCAGCACCACTCTCGTGGTCTTCGGCGGTGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG22540.17647952320776197No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22210.17389575024154363No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGTTTATAACTATGTCTCCTGGTACCAACAGCACCCCGGCAAAGC22080.17287790028515457No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21830.17092049652286795No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20630.16152495846389217No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20500.16050710850750313No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT20150.15776674324030185No Hit
CCTCTGAGGACCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20140.1576884470898104No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19790.1549480818226091No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGGAGCTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG19550.15306897421081395No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19400.151894531953442No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18810.14727505907444555No Hit
ACCTAGGACGGTCAGCTTGGTCCCACCGCCGAAGACCACGAGAGTGGTGC18690.14633550526854797No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18640.14594402451609062No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGTTTATAACTATGTCTCCTGGTACCAACAGCA18250.1428904746469235No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18010.14101136703512834No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17560.13748804026301242No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17510.1370965595105551No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGGTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17510.1370965595105551No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17380.13607870955416607No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17260.13513915574826849No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17230.13490426729679408No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16910.1323987904810672No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16840.13185071742762694No Hit
GTTTATAACTATGTCTCCTGGTACCAACAGCACCCCGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAT16590.12989331366534035No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16480.1290320560099342No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16280.12746613300010493No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16280.12746613300010493No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16200.1268397637961732No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15790.12362962162602313No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15730.12315984472307435No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15680.12276836397061702No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCAC15610.12222029091717677No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15320.1199497025529243No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15180.11885355644604377No Hit
CTCTGTTCCCGCCCTCCTCTGAGGACCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14480.11337282591164122No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14430.11298134515918391No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14420.11290304900869244No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14280.11180690290181193No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT14210.11125882984837168No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14130.11063246064443996No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13600.10648276466839232No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGGTCCTCAGAGGA13580.10632617236740938No Hit
GTTCCCGCCCTCCTCTGAGGACCTTCAAGCCAACAAGGCCACACTGGTGT13570.10624787621691793No Hit
CAGTAATATACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATA13540.10601298776544352No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13450.10530832241102035No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13330.10436876860512274No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13320.1042904724546313No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG13310.10421217630413983No Hit
CCTTTAGGAGCTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG13310.10421217630413983No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13250.10374239940119104No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGGCATAGCTCC13240.10366410325069958No Hit
GATTATTACTGCAGTTCATATACAGGCAGCACCACTCTCGTGGTCTTCGG13200.10335091864873372No Hit
GGTACCAACAGCACCCCGGCAAAGCCCCCAAACTCATGATTTATGAGGTC12960.10147181103693857No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGGAGCTATGCCATGAACTGGGTCCG12860.10068884953202391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)