FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277202
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA46150.3613367345181107No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGACC38540.30175336399410585No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCACCGCCGAAGACCACGAGAGTGGTGCTGCCTGTATATGAA33320.26088277343756117No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG32980.25822070432085137No Hit
GTATATTACTGTGCGAAAGAGACTATAGCAGATCGCCTTGGGGCTTATGA31300.24506695103828527No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGACCTTCAAGCCAACAAGG31030.2429529549750157No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30040.23520163607636066No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT28440.22267425199772628No Hit
GACCTAGGACGGTCAGCTTGGTCCCACCGCCGAAGACCACGAGAGTGGTG28200.22079514438593115No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24730.1936263801653928No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24710.19346978786440985No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24530.1920604571555635No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23660.18524869206280603No Hit
GTTCATATACAGGCAGCACCACTCTCGTGGTCTTCGGCGGTGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG23570.18454402670838285No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23510.18407424980543408No Hit
GAATAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT23380.183056399849045No Hit
GGACTGAATAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT23220.18180366144118157No Hit
GTATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA23080.18070751533430107No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGTTTATAACTATGTCTCCTGGTACCAACAGCACCCCGGCAAAGC22700.17773226161562541No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20890.16356065837667025No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20800.16285599302224707No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGGAGCTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG20190.1580799278422677No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20020.1567488932839128No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19980.15643570868194695No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT19910.1558876356285067No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19630.15369534341474567No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19170.15009372049213826No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18910.1480580205793602No Hit
CCTCTGAGGACCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18630.1458657283655992No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18520.14500447071019307No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18430.1442998053557699No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18180.14234240159348327No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17820.1395237401757905No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17580.13764463256399534No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGTTTATAACTATGTCTCCTGGTACCAACAGCA17410.13631359800564047No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17260.13513915574826849No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16780.13138094052467816No Hit
GTTTATAACTATGTCTCCTGGTACCAACAGCACCCCGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAT16490.12911035216042568No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG16480.1290320560099342No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16360.12809250220403662No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16070.12582191383978414No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15670.12269006782012555No Hit
ACCTAGGACGGTCAGCTTGGTCCCACCGCCGAAGACCACGAGAGTGGTGC15620.12229858706766822No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15450.12096755250931333No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15370.12034118330538161No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15260.11947992564997549No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15210.11908844489751816No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGGTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15020.11760081803818033No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14460.1132162336106583No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14390.11266816055721805No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14380.11258986440672658No Hit
CTCTGTTCCCGCCCTCCTCTGAGGACCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14120.11055416449394849No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13960.10930142608608505No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGGCATAGCTCC13720.1074223184742899No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13690.1071874300228155No Hit
GTTCCCGCCCTCCTCTGAGGACCTTCAAGCCAACAAGGCCACACTGGTGT13650.10687424542084964No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13420.10507343395954594No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13320.1042904724546313No Hit
GATTATTACTGCAGTTCATATACAGGCAGCACCACTCTCGTGGTCTTCGG13300.10413388015364836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13180.10319432634775079No Hit
CTCCAGGGACAATTCCAAGGACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGACGACACGGCCGTATATTA13170.10311603019725932No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCAC13010.10186329178939588No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13010.10186329178939588No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGGAGCTATGCCATGAACTGGGTCCG12950.1013935148864471No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12880.10084544183300682No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12840.10053225723104096No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12830.10045396108054952No Hit
CCTTTAGGAGCTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG12820.10037566493005805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA200.00723395151.9751644
GTGGTAT2800.042.6968351
GGTATCA8800.038.8153041
GTATCAA21850.037.3621751
TGGTATC3600.032.2509882
TATCAAC25750.031.429522
ATCAACG26050.031.0651343
AATGGTA4350.030.80588770
TCAACGC26300.030.6388074
CAACGCA26450.030.5965375
CCTCGTA450.00483830530.3824239
AACGCAG27400.029.797796
CGCAGAG27750.028.8188578
AGAGTAC27900.028.79882811
CAGAGTA28300.028.14462910
GCAGAGT29050.027.7677569
GCCGTTA500.008085527.35061315
CTCTAAC500.00812113127.3259751
GAGTTTA4450.026.0978431
GAGTACT10300.024.89729512
Produced by FastQC (version 0.11.5)