FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277643
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG48910.38281429162919534No Hit
GTAGTACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA46940.36739527395367877No Hit
CAGTAGTACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATA36300.2841169246808381No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG35220.27566385915314373No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA33680.2636104138636536No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30310.23723371865223697No Hit
GTGTACTACTGTGCGCGACACGCTAGGCCCTCGTATGATGGGAGCTACTACTGGCTATATTTTGACTCCTGGGGC29950.2344160301430055No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT28950.22658911761736258No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28900.22619777199108046No Hit
CTTCTATACTGACTATTACATGACCTGGGTCCGCCAGGCTCCAGGGGCGG28690.22455412036069544No Hit
GTATAATAACTGGCCTCCCAGAGTCTCTTTCGGCCCTGGGACCAAAGTGG28420.22244085397877186No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT28100.2199362419705661No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26970.21109183081658964No Hit
GTTATTATACTGCTGACAGTAATAAACCGCAAAATCTTCAGACTCCAGGCTGCTGATGGTGAGCGTGAACT25530.19982107677966382No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24880.19473358363799592No Hit
GTGCAGGGCCGATTCACCATCTCCAGAGACAACACCAAGAACTCACTGTA24130.18886339924376372No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC23870.18682840198709655No Hit
CTCCAGCCCCGCCCCTGGAGCCTGGCGGACCCAGGTCATGTAATAGTCAG23470.18369763697683938No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC22770.17821879820888933No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC22390.17524457144914501No Hit
GTACTACTGTGCGCGACACGCTAGGCCCTCGTATGATGGGAGCTACTACT21740.17015707830747714No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21480.16812208105080997No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21190.16585227641837352No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT20200.15810363301798702No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19660.15387710025413984No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGTCAGAAGTTCTA19340.15137248824593413No Hit
GCCCTGCACAGAGTCCGCATAGAACTTCTGACTTCCATCTTGCTTTATGTTGGCCACCCACTCCAGCCCCGCCC19300.1510594117449084No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC18690.1462849951042662No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18310.14331076834452192No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC18010.14096269458682903No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17860.1397886577079826No Hit
GATCAGCACTGAGCACAGAAGACTCACCATGGAATTGGGGCTGAGCTGGG17610.13783192957657187No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT17600.13775366045131543No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17250.13501424106734042No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT17200.13462289544105827No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16890.13219655255810894No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16860.13196174518233966No Hit
GTCCCAGCCCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT16850.13188347605708323No Hit
CTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGCACAGAAGACTCACC16740.1310225156792625No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC16620.13008328617618536No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16560.1296136714246468No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16150.1264046372891332No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG15760.12335214140413245No Hit
GCCTTAGCCCTGGACTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGC15700.12288252665259387No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC15500.12131714414746529No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC15460.12100406764643956No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15270.11951695426656742No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT15010.11748195700990026No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA14990.11732541875938741No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14980.11724714963413096No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14970.11716888050887454No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14900.11662099663207953No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCATCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT14630.11450773025015595No Hit
CTCCTGTGCAGCCTCTCGATTCTTCTATACTGACTATTACATGACCTGGGTCCGCCAGGCTCCAGGGGCGGGG14530.11372503899759166No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGGCTGGGACAGAGTTCACG14450.11309888599554023No Hit
GTCTCCAGGCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAT14390.11262927124400166No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT14390.11262927124400166No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTG14250.11153350349041163No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC14080.11020292836105233No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14050.10996812098528305No Hit
CTCCAGGCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13930.1090288914822059No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA13780.10785485460335947No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG13750.10762004722759018No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT13730.10746350897707732No Hit
GGCCGGTACTTCGCCCACAGCATCCTGACCGTGTCCGAAGAGGAATGGAA13720.1073852398518209No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG13590.1063677412234873No Hit
GCCCTGGACTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGCACAGAAGACTCACCATGGAATTGGGGCTGAG13470.10542851172041015No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13290.10401966746579443No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC13270.10386312921528157No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13240.1036283218395123No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG13220.10347178358899943No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC13140.102845630586948No Hit
GTAATAAACCGCAAAATCTTCAGACTCCAGGCTGCTGATGGTGAGCGTGAACTCTGTCCCAGCCCCACTGCCA13100.10253255408592228No Hit
ACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGG13010.10182813195861443No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA13000.10174986283335799No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT12910.10104544070605012No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAATATA12900.1009671715807937No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG12860.10065409507976797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACC200.0048739657.4303467
GGGCGTA200.00817889650.385855
CGTAAAC306.94773E-445.112332
ACCGCAC359.183678E-442.6268864
CTTATAC3000.042.5497171
GGTATCA5050.041.9067041
GCCGTCT3300.041.197546
CCGTCTT3350.040.71988747
TTATACA3150.040.5219542
CGTATGC3150.040.469341
GTATGCC3200.039.91869742
ACGAGAC3300.039.71348622
TATACAC3300.039.6979453
CTACATC3100.039.3332333
TCTGGAG16600.038.91079370
GTCGTAA350.001499217438.58402630
ATCTCGT3200.038.37057537
TACACAT3650.037.731875
CACGAGA3500.037.4382821
TCTACAT3400.036.81960332
Produced by FastQC (version 0.11.5)