FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277643
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG47260.3698998859618845No Hit
GTAGTACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA42510.3327220514650806No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40910.32019899142405195No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA32330.25304408195403566No Hit
GTATAATAACTGGCCTCCCAGAGTCTCTTTCGGCCCTGGGACCAAAGTGG32250.2524179289519842No Hit
CAGTAGTACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATA31510.24662601368300846No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29820.23339853151467194No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29510.23097218863172264No Hit
GTGTACTACTGTGCGCGACACGCTAGGCCCTCGTATGATGGGAGCTACTACTGGCTATATTTTGACTCCTGGGGC28560.22353662173236186No Hit
CTTCTATACTGACTATTACATGACCTGGGTCCGCCAGGCTCCAGGGGCGG28160.22040585672210466No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26960.2110135616913332No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT26010.20357799479197242No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25930.20295184178992098No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC25080.19629896614312448No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24820.19426396888645733No Hit
GTTATTATACTGCTGACAGTAATAAACCGCAAAATCTTCAGACTCCAGGCTGCTGATGGTGAGCGTGAACT24600.19254204813081588No Hit
GTGCAGGGCCGATTCACCATCTCCAGAGACAACACCAAGAACTCACTGTA24070.18839378449222513No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC24010.18792416974068657No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23740.185810903358763No Hit
CTCCAGCCCCGCCCCTGGAGCCTGGCGGACCCAGGTCATGTAATAGTCAG23240.1818974470959415No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22270.17430534194606787No Hit
GTACTACTGTGCGCGACACGCTAGGCCCTCGTATGATGGGAGCTACTACT20170.15786882564221774No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC19620.15356402375311412No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19560.15309440900157556No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19370.1516072956217034No Hit
GCCCTGCACAGAGTCCGCATAGAACTTCTGACTTCCATCTTGCTTTATGTTGGCCACCCACTCCAGCCCCGCCC19120.14965056749029268No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC18530.14503268910016334No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC18280.1430759609687526No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGTCAGAAGTTCTA18140.14198019321516261No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18090.14158884758888046No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC17230.13485770281682755No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16350.12797001979426179No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16310.12765694329323604No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT16020.12538713866079962No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15870.12421310178195318No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT15700.12288252665259387No Hit
CTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGCACAGAAGACTCACC15680.12272598840208102No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC15640.1224129119010553No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15600.1220998354000296No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT15600.1220998354000296No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15580.12194329714951672No Hit
GCCTTAGCCCTGGACTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGC15450.12092579852118314No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15420.12069099114541387No Hit
CTCCTGTGCAGCCTCTCGATTCTTCTATACTGACTATTACATGACCTGGGTCCGCCAGGCTCCAGGGGCGGGG15390.12045618376964456No Hit
GATCAGCACTGAGCACAGAAGACTCACCATGGAATTGGGGCTGAGCTGGG15330.11998656901810599No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCATCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT15240.11928214689079814No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT15210.11904733951502884No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15110.11826464826246456No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA14930.11685580400784884No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG14810.11591657450477169No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC14770.11560349800374596No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14720.11521215237746381No Hit
GTCCCAGCCCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT14670.11482080675118166No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC14540.11380330812284808No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA14420.11286407861977094No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG14400.11270754036925808No Hit
GTCTCCAGGCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAT14200.11114215786412948No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14090.11028119748630878No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGGCTGGGACAGAGTTCACG13990.10949850623374448No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13750.10762004722759018No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13700.10722870160130803No Hit
CTCCAGGCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13670.10699389422553875No Hit
GGCCGGTACTTCGCCCACAGCATCCTGACCGTGTCCGAAGAGGAATGGAA13630.10668081772451303No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13590.1063677412234873No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13470.10542851172041015No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13410.10495889696887158No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA13340.10441101309207658No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC13180.10315870708797373No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13110.1026108232111787No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT13050.10214120845964013No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGAGACTCTGGGAGGCCAGTTATTATA13050.10214120845964013No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12780.10002794207771655No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTG12780.10002794207771655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5450.046.156661
TCTGGAG14700.043.3862770
GTATAAG551.8345963E-742.687921
TGCATAG750.001570250338.21891470
GTATCAA12950.037.5550081
AGAGCCG11650.035.0613470
AATCGTT400.002681737334.2732739
TTAATCG400.00274278834.11628337
GCTTGTA601.6286516E-533.540511
TAGCGGC450.003143413233.1801364
TATCAAC14900.032.6247632
GCTCGGG5800.032.12365770
CGAACCT450.00392397231.7111453
ATCAACG15850.030.6681333
TCAACGC15850.030.6681334
TAATCGT450.004832581630.38973638
CAACGCA16050.030.2883475
AACGCAG16350.029.943516
AGAGTAC16500.029.49000511
CTCTGGA14800.029.35845669
Produced by FastQC (version 0.11.5)