FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954612
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAACTCAACTTCAGGGAGA50390.5278584388212174No Hit
GTATATTACTGTGCGCGACATCCGTATCTAGCAGCAACTGAGGGCGACTGGTTCGACCCCTGGGGCCAGGGAACC42850.44887346901149366No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37490.3927250024093558No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32330.33867162784461124No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGCATTTACTACTGGGGCTGGATCCG29670.31080690374728165No Hit
GAGTTACAGTACGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT28080.29415092205000565No Hit
CAGTAATATACAGCCGTGTCTGCGGCGGTCACAGAACTCAACTTCAGGGA26890.2816851244275161No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25510.267228989369503No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24550.2571725475900156No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24070.2521443267002719No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22790.23873573766095543No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22720.23800245544786783No Hit
ATATTACTGTGCGCGACATCCGTATCTAGCAGCAACTGAGGGCGACTGGTTCGACCCCTGGGGCCAGGGAACC22540.23611687261421394No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGCATTTACTACTGGG21960.23004110570577366No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21170.22176549215807048No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20980.21977515472254697No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20960.21956564551880764No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC20720.21705153507393582No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20570.2154802160458909No Hit
GATCTATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA20520.2149564430365426No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20480.21453742462906397No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19940.2088806761281023No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19780.20720460249818773No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGCAC18470.19348174965326226No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18250.19117714841212974No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18100.18960582938408485No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17870.18719647354108268No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16940.17745429556720427No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16840.17640674954850766No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16620.17410214830737514No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16580.1736831298998965No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16240.17012147343632805No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16140.16907392741763147No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16020.16781687219519553No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15960.16718834458397758No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15950.1670835899821079No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15930.1668740807783686No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15860.16614079856528097No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15770.16519800714845403No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA15550.16289340590732151No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15450.1618458598886249No Hit
ACATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT15350.1607983138699283No Hit
GGCCAAAAGTGTACGGAGGCGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTG15080.15796993961944747No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14880.15587484758205428No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCCTATCCGTCGACACGTCC14650.1534654917390521No Hit
ATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA14650.1534654917390521No Hit
ACTTACTACTGTCAACAGAGTTACAGTACGCCTCCGTACACTTTTGGCCA14440.15126564509978924No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14410.15095138129418026No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14350.1503228536829623No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14340.15021809908109263No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14110.14780874323809043No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13930.14592316040443656No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13690.14340904995956472No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13600.14246625854273778No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13510.14152346712591085No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13360.13995214809786594No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13090.1371237738473851No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGCACCTATTTAAATTGGTATCAGCAGAAA12950.13565720942120987No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12880.13492392720812224No Hit
AGTCAGAGCATTGGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAA12860.1347144180043829No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12730.13335260818007735No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12720.1332478535782077No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12590.13188604375390212No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12540.1313622707445538No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12270.12853389649407299No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACG12150.12727684127163708No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCGCCCT12070.1264388044566798No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12070.1264388044566798No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG12010.1258102768454618No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG11980.12549601303985283No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11960.1252865038361135No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11870.12434371241928659No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11860.12423895781741691No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11710.12266763878937201No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA11510.12057254675197883No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGACTCCTGCAGCTGCAG11500.12046779215010915No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11390.11931549152954289No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11340.1187917185201946No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11230.11763941789962834No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11150.11680138108467106No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11110.11638236267719242No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACGCCTCCGTACA11040.11564908046410478No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT10900.11418251603792955No Hit
GTGTACGGAGGCGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATC10770.11282070621362397No Hit
CCCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCCTATCCGTCGACAC10670.11177316019492736No Hit
GTACGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA10320.10810674912948925No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC10220.10705920311079266No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10180.10664018470331403No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10140.10622116629583538No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10130.10611641169396573No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10090.10569739328648707No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10070.10548788408274776No Hit
TCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG10020.10496411107339945No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9970.10444033806405115No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9840.10307852823974557No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9720.10182147301730966No Hit
GAGGCGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGT9710.10171671841543999No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGTCGCCCTCAGTTGCTGCTAGATACGGATGTCGCGCACAGTAATATA9600.10056441779487374No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA9570.10025015398926476No Hit
GTAGTAGGGGCTCCCACGAGAAGAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC9550.10004064478552543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAT12000.056.0736270
GTGGTAT1850.055.4526021
GTTCTAA250.007429199351.62423769
CGTTCTC200.00759095651.3447627
GCCGTCT503.6593374E-641.69449246
ATGCCGT503.7281752E-641.58162744
CGTATGC503.81473E-641.44304341
CATAAGC503.9901006E-641.17295532
GTATCAA12950.038.0246431
CGACGGA12000.036.9256769
ATCTCGT601.3634999E-534.42114637
TAGTACA501.9606069E-434.1957744
TATCAAC14850.032.698992
TCGTATG652.3197066E-531.84893240
TCAACGC15950.030.658284
TAGATTC450.004807230.42173826
AATAGAT450.00481583530.4105824
CGATCGG450.004825718230.39783315
GTATAGT450.00482695530.3962441
ATCAACG16100.030.3726433
Produced by FastQC (version 0.11.5)