FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469985
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAGATCAGACCATCCAGG85860.584087592730538No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAGATCAGACCATCCAGGTTGTCATCCCAAA77110.5245631758147192No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG74610.5075561995530566No Hit
GTCCTGGGCCCAGTCTGTGGTGACTCAGTCACCCTCAGCGTCTGGGACCC74370.505923529831937No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG73260.49837243237175893No Hit
GATTATTACTGTGCAGTTTGGGATGACAACCTGGATGGTCTGATCTTCGG72590.49381456273363333No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC71930.4893247210005544No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGTCACCCTCAGCGTCT63980.43524253648846756No Hit
GTAATAGACGGCCGTGTCGTCGGCTCTCAGGTTGTTCATTTGCAGAAACA57490.3910924261131916No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53720.36544590591060455No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC47760.32490127450280104No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45590.31013921910767794No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC44560.303132344887873No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGTCAC42010.28578522910097726No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC41910.2851049500505107No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAGATCAGACCATCCAGGT40320.2742885131480933No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT40210.2735402061925802No Hit
GTCTGTGGTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGG38140.2594584298479236No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG38040.25877815079745714No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG36870.2508188859069991No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG36830.25054677428681243No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35980.2447644023578472No Hit
CCCCTGAGGGCCGCTGAGTATTCCTATAGATGAGCAACTTGGGGGCACTTCCTGGGAGAAGCTGGTACCAATTTA34870.237213304897669No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT34170.2324513515444035No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC33990.23122684925356382No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA33530.22809756562141792No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC32260.21945802168049333No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG31480.21415184508685464No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA31090.21149875679003527No Hit
GAGTATTCCTATAGATGAGCAACTTGGGGGCACTTCCTGGGAGAAGCTGG29590.20129457103303774No Hit
GTGGTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCAC29220.1987775385463117No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA28830.19612445024949232No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28790.19585233862930573No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT28310.19258699918706654No Hit
GTCTTGTTCTGGAAGCAGGTCCAACATCAAAGACAATAATGTAAATTGGTACCAGCTTCTCCCAGGAAGTGCCCC28120.1912944689911802No Hit
GTATTCCTATAGATGAGCAACTTGGGGGCACTTCCTGGGAGAAGCTGGTA28000.19047813413062037No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27780.18898152021959408No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26890.18292703667044222No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT26510.1803419762786695No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAGAT25980.17673649731119706No Hit
ATAGGAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG25940.17646438569101044No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25560.17387932529923775No Hit
TCTATAGGAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC25500.17347115786895786No Hit
CTCCTGTGCAGTCTCAGGCTTCACATTTAGTAACTTTGCCATGGGCTGGG25020.17020581842671864No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG25000.17006976261662532No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG24740.16830103708541244No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24480.16653231155419954No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24360.16571597669363972No Hit
GTTTGGGATGACAACCTGGATGGTCTGATCTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG24240.16489964183307992No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC23760.16163430239084073No Hit
ATCCCAAACTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCAC23650.16088599543532756No Hit
CTTCCTGGGAGAAGCTGGTACCAATTTACATTATTGTCTTTGATGTTGGA23420.15932135361925462No Hit
CCTTGGTCCCTCCGCCGAAGATCAGACCATCCAGGTTGTCATCCCAAACTGCACAGTAATAATCACCCTCATCCT23180.157688683898135No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22640.1540151770256159No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22380.152246451494403No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22210.15108997710860994No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT22160.1507498375833767No Hit
CCCAGGAAGTGCCCCCAAGTTGCTCATCTATAGGAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG22120.1504777259631901No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC21980.149525335292537No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC21780.148164777191604No Hit
GGAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG21630.14714435861590425No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCACCATGTCTTGTTCTGG21570.14673619118562434No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21440.14585182842001787No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21330.14510352146450475No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21290.14483140984431814No Hit
GTCTATTACTGTGCGAAAGATGGTTTTAGGGTCGTGGGAGCTACAATGGGGGCTTTTGATACCTGGGGCCAAGGG21050.14319874012319853No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20880.1420422657374055No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGGTGATTATTACTGTGCAGTTTGGGATGACAACCTGGATGGT20660.14054565182637918No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20400.13877692629516628No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20250.13775650771946651No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG20150.13707622866900002No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20100.13673608914376678No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20020.13619186590339358No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20020.13619186590339358No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG19940.13564764266302037No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19730.13421905665704073No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19130.13013738235424172No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGTCACCCTCAGCGTCTGGGA18980.12911696377854195No Hit
GTCTATTACTGTGCGAAAGATGGTTTTAGGGTCGTGGGAGCTACAATGGG18760.12762034986751566No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT18760.12762034986751566No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18470.12564754062116282No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC18070.1229264244192968No Hit
GTCTCAGGCTTCACATTTAGTAACTTTGCCATGGGCTGGGTCCGCCAGGCTCCAGGCAAGGGCCCGGAGTGGGT17850.12142981050827048No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG17750.12074953145780398No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG17630.11993319659724419No Hit
GTCTGGGACCCCCGGGCAGGGGGTCACCATGTCTTGTTCTGGAAGCAGGT17270.11748419201556479No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT17240.11728010830042485No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17110.11639574553481838No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC17060.11605560600958513No Hit
GTCTGATCTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC16840.11455899209855884No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16640.11319843399762583No Hit
AGTCTGTGGTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGGGG16600.11292632237743921No Hit
AGATCAGACCATCCAGGTTGTCATCCCAAACTGCACAGTAATAATCACCC16600.11292632237743921No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCAC16580.11279026656734593No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16570.11272223866229927No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16310.11095351313108637No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG16220.11034126198566652No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTG16100.10952492712510671No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16070.10932084340996677No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16030.10904873178978017No Hit
TTCCTATAGATGAGCAACTTGGGGGCACTTCCTGGGAGAAGCTGGTACCA15890.10809634111912705No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15860.10789225740398711No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15670.10659972720810076No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15630.10632761558791416No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC15440.1050350853920278No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15340.1043548063415613No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15310.10415072262642136No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15290.10401466681632807No Hit
GTCAGGGACCCCTGAGGGCCGCTGAGTATTCCTATAGATGAGCAACTTGGGGGCACTTCCTGGGAGAAGCTGGTA15260.10381058310118811No Hit
CATCTATAGGAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC15220.10353847148100151No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGTCACCCTCAG15040.1023139691901618No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14900.1013615785195087No Hit
CCTGAGGGCCGCTGAGTATTCCTATAGATGAGCAACTTGGGGGCACTTCC14780.1005452436589489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)