FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469985
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGGTGACTCAGTCACCCTCAGCGTCTGGGACCC83740.5696656768606483No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGTCACCCTCAGCGTCT72590.49381456273363333No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAGATCAGACCATCCAGG70500.4795967305788834No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC70050.47653547485178416No Hit
GATTATTACTGTGCAGTTTGGGATGACAACCTGGATGGTCTGATCTTCGG68940.46898437739160603No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAGATCAGACCATCCAGGTTGTCATCCCAAA68820.46816804253104627No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG64870.44129702003761945No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG57110.3885073657214189No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC47600.32381282802205463No Hit
GTAATAGACGGCCGTGTCGTCGGCTCTCAGGTTGTTCATTTGCAGAAACA47290.3217039629656085No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC43890.29857447524974745No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGTCAC42560.289526763878543No Hit
GTCTGTGGTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGG42420.28857437320788987No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42060.28612536862621046No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT41530.282519889658738No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA40620.27632935029949285No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG39830.2709551458008075No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG38050.25884617870250376No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC36130.24578482093354695No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG35920.24435623492756728No Hit
GTGGTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCAC35750.24319976054177425No Hit
GTCTTGTTCTGGAAGCAGGTCCAACATCAAAGACAATAATGTAAATTGGTACCAGCTTCTCCCAGGAAGTGCCCC35220.23959428157430177No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT34410.23408402126552313No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34030.23149896087375038No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33020.22462814246403875No Hit
CCCCTGAGGGCCGCTGAGTATTCCTATAGATGAGCAACTTGGGGGCACTTCCTGGGAGAAGCTGGTACCAATTTA32810.2231995564580591No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAGATCAGACCATCCAGGT32220.21918591006030674No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC32000.21768929614928043No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31640.21524029156760102No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG31570.21476409623227447No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAGAT31000.21088650564461542No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG29590.20129457103303774No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29390.19993401293210475No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC29370.19979795712201145No Hit
GTATTCCTATAGATGAGCAACTTGGGGGCACTTCCTGGGAGAAGCTGGTA28520.19401558519304618No Hit
ATAGGAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG28150.19149855270632013No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT27890.18972982717510722No Hit
GAGTATTCCTATAGATGAGCAACTTGGGGGCACTTCCTGGGAGAAGCTGG27290.1856481528723082No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG27120.18449167848651515No Hit
TCTATAGGAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC26700.18163450647455587No Hit
GTCTATTACTGTGCGAAAGATGGTTTTAGGGTCGTGGGAGCTACAATGGG26540.18054605999380946No Hit
CTCCTGTGCAGTCTCAGGCTTCACATTTAGTAACTTTGCCATGGGCTGGG25840.17578410664054395No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC25580.17401538110933104No Hit
GTTTGGGATGACAACCTGGATGGTCTGATCTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG24710.16809695337027247No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24680.16789286965513253No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24660.16775681384503924No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24050.16360711163719358No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24010.16333500001700696No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT23800.16190641401102732No Hit
CTTCCTGGGAGAAGCTGGTACCAATTTACATTATTGTCTTTGATGTTGGA23010.15653220951234195No Hit
CCCAGGAAGTGCCCCCAAGTTGCTCATCTATAGGAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG22670.15421926074075587No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22600.1537430654054293No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22500.1530627863549628No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22380.152246451494403No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22200.15102194920356332No Hit
GGAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG22140.15061378177328338No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT21580.14680421909067098No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21460.1459878842301112No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21190.14415113079385164No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGGTGATTATTACTGTGCAGTTTGGGATGACAACCTGGATGGT21170.14401507498375835No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21100.1435388796484318No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21040.1431307122181519No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG20920.1423143773575921No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG20780.141361986686939No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20660.14054565182637918No Hit
CCTTGGTCCCTCCGCCGAAGATCAGACCATCCAGGTTGTCATCCCAAACTGCACAGTAATAATCACCCTCATCCT20530.13966128906077274No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC20380.13864087048507298No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20280.13796059143460646No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20010.13612383799834693No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19900.13537553104283376No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC19850.13503539151760052No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT19740.13428708456208735No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC19350.131633996265268No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT19130.13013738235424172No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19120.13006935444919507No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG18980.12911696377854195No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCAC18910.1286407684432154No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGTCACCCTCAGCGTCTGGGA18810.1279604893927489No Hit
GTCTGGGACCCCCGGGCAGGGGGTCACCATGTCTTGTTCTGGAAGCAGGT18590.1264638754817226No Hit
GTCTCAGGCTTCACATTTAGTAACTTTGCCATGGGCTGGGTCCGCCAGGCTCCAGGCAAGGGCCCGGAGTGGGT18050.1227903686092035No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17790.1210216430779906No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17490.11898080592659109No Hit
GTCTGATCTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC17440.11864066640135784No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17360.11809644316098464No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17320.11782433154079804No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGTCACCCTCAG17110.11639574553481838No Hit
ATCCCAAACTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCAC16900.11496715952883872No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC16760.11401476885818562No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC16490.11217801542192608No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTG16460.11197393170678611No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG16350.11122562475127298No Hit
AGTCTGTGGTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGGGG16220.11034126198566652No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16180.11006915036547993No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG16090.10945689922006008No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCACCATGTCTTGTTCTGG16080.10938887131501342No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16070.10932084340996677No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15990.10877662016959358No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15930.10836845273931367No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC15890.10809634111912705No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15800.1074840899737072No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15750.10714395044847395No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15730.10700789463838067No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC15700.1068038109232407No Hit
CATCTATAGGAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC15310.10415072262642136No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15220.10353847148100151No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15150.10306227614567495No Hit
CTCCCAGGAAGTGCCCCCAAGTTGCTCATCTATAGGAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCT15100.1027221366204417No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCACCATGTCTTGTTCTGGAAGCAGGTCCAACATCAAAGACAAT15050.10238199709520845No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14990.10197382966492854No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC14970.10183777385483525No Hit
GTCCGAGGATGAGGGTGATTATTACTGTGCAGTTTGGGATGACAACCTGGATGGTCTGATCTTCGGCGGAGGGAC14780.1005452436589489No Hit
GTCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCACCATGTCTTGTTCTGGAAGCAGGTCCAACATCAAAGA14750.10034115994380895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT100.009055604133.6063770
TCTGGAG14350.055.86329370
GTGGTAT2550.041.5220261
CTCTGGA15600.039.7004369
TATATCG350.001417815939.0252345
GTATAAG1350.037.9502371
CAAGGGA12500.037.40978270
TGGTATC2850.037.1348572
TATCAAC28300.036.3115732
ATTTGCA14250.035.31499538
ATCAACG29450.034.8924453
TCAACGC29550.034.889894
GGGTGTT14550.034.84781651
GGTGTTC14400.034.7639152
GTGTTCT14450.034.68959453
AACGCAG29850.034.670126
GGCGCTA400.00255682634.60865842
TGTCGTC14350.034.54280515
TTGCAGA14600.034.53838740
GAATTGT14850.034.52665362
Produced by FastQC (version 0.11.5)