FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711704
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26670.37473444016051616No Hit
GCCTTGGGCTGACCGACGACGGTCAGTCTGGTCCCTCCGCCGAAAGCCGC20780.2919753155806346No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15970.22439103897125773No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14770.20753009678180817No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14490.20359587693760325No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14170.19909962568708342No Hit
CCCCTGTCCTGCTCCTCTATCAAGATAAGCAGCGGCCCTCAGGGATCCCT13380.18799950541236243No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11610.16312961568292436No Hit
GGACAGGGGACTGGCCTGGTTTTTGCTGATACCACGAAACAAAGTTATCC11480.16130301361240068No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10870.15273203466609714No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGACGACGGTCAGTCTGGTCCCTC10760.15118644829873093No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10640.14950035407978599No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10380.1458471499387386No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9930.139524296617695No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9870.13868124950822253No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9850.13840023380506503No Hit
GTCTGGTCCCTCCGCCGAAAGCCGCAATGTGGCTGGCCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGG9180.12898620774928904No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT8950.12575452716297786No Hit
TCCATAGGCTGGGTTCCGCTAATGGTCAGAGTGGCTGTGTTCCCAGAACTGGACCCAGAGAATCGCTCAGGGATC8940.12561401931139912No Hit
GTCCTGCTCCTCTATCAAGATAAGCAGCGGCCCTCAGGGATCCCTGAGCG8930.12547351145982039No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8910.12519249575666289No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8900.12505198790508412No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8750.12294437013140294No Hit
CCCTCAGTGTCCGTGTCACCAGGACACACAGCCAACATCACCTGCTCTGG8700.1222418308735092No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT8610.1209772602093005No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG8400.11802659532614683No Hit
GACTATTACTGTCAGGCGTGGGCCAGCCACATTGCGGCTTTCGGCGGAGGGACCAGACTGACCGTCGTCGGTCAG8330.11704304036509561No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8230.11563796184930814No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACCGTCACGGCTC8180.11493542259141441No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACGGTGGC8160.11465440688825691No Hit
GAGTGGCTGTGTTCCCAGAACTGGACCCAGAGAATCGCTCAGGGATCCCT8120.11409237548194193No Hit
GCCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGCCAGCCACATTGCGGCTTTCGGCGGAGGGACCAGA8030.1128278048177332No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8020.11268729696615448No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA7800.10959612423142205No Hit
CTATTACTGTCAGGCGTGGGCCAGCCACATTGCGGCTTTCGGCGGAGGGACCAGACTGACCGTCGTCGGTCAGCC7670.10776952216089836No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7660.1076290143093196No Hit
GTAATAGTCAGCCTCATCCATAGGCTGGGTTCCGCTAATGGTCAGAGTGG7590.10664545934826838No Hit
GAGCAGGACAGGGGACTGGCCTGGTTTTTGCTGATACCACGAAACAAAGT7580.10650495149668963No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7560.10622393579353213No Hit
GGACCAGACTGACCGTCGTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG7460.10481885727774468No Hit
CAGCCACTCTGACCATTAGCGGAACCCAGCCTATGGATGAGGCTGACTAT7450.10467834942616593No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACGGTGGCCTGGA7220.10144666883985477No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7210.10130616098827602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC851.8189894E-1244.81685341
TCGTATG851.8189894E-1244.72079540
GTCGTAT405.0145274E-543.10383639
CGAGACA1250.038.0647323
TACGTAT851.2646186E-436.83659770
TCTCCGA1300.033.98398211
TGACAGT8600.033.49560570
GTATCAA22800.033.2410551
TATCAAC22900.032.9474872
ACGAGAC1350.032.72531522
CACGAGA1253.6379788E-1232.6223321
CCCACGA1253.6379788E-1232.6223319
ATCAACG23150.032.1512533
TCAACGC23350.031.8758684
TCTCGTA553.2994055E-431.2924738
CAACGCA23900.031.0001185
CGGCTAG450.0045364430.78329341
GCGGCAT604.0489883E-430.21803364
AACGCAG24500.030.1022116
TATACAC1750.029.1311743
Produced by FastQC (version 0.11.5)