FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711704
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25320.3557658801973855No Hit
GCCTTGGGCTGACCGACGACGGTCAGTCTGGTCCCTCCGCCGAAAGCCGC18510.26008003327225926No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15630.21961377201758034No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14900.20935669885233185No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14750.20724908107865067No Hit
CCCCTGTCCTGCTCCTCTATCAAGATAAGCAGCGGCCCTCAGGGATCCCT13930.1957274372491935No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13250.18617290334183872No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13230.18589188763868125No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11100.1559637152524083No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10740.15090543259557343No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10030.14092937513348247No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9700.13629261603138385No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9650.1355900767734901No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9470.13306093544507266No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9250.12996976271034025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9050.12715960567876533No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGACGACGGTCAGTCTGGTCCCTC8980.1261760507177141No Hit
GTCCTGCTCCTCTATCAAGATAAGCAGCGGCCCTCAGGGATCCCTGAGCG8890.1249114800535054No Hit
CCCTCAGTGTCCGTGTCACCAGGACACACAGCCAACATCACCTGCTCTGG8870.1246304643503479No Hit
GTCTGGTCCCTCCGCCGAAAGCCGCAATGTGGCTGGCCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGG8860.12448995649876915No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT8860.12448995649876915No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT8840.12420894079561166No Hit
GGACAGGGGACTGGCCTGGTTTTTGCTGATACCACGAAACAAAGTTATCC8840.12420894079561166No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG8790.12350640153771793No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8400.11802659532614683No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8380.11774557962298934No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8190.11507593044299315No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACCGTCACGGCTC8160.11465440688825691No Hit
GCCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGCCAGCCACATTGCGGCTTTCGGCGGAGGGACCAGA8020.11268729696615448No Hit
GACTATTACTGTCAGGCGTGGGCCAGCCACATTGCGGCTTTCGGCGGAGGGACCAGACTGACCGTCGTCGGTCAG7830.1100176477861583No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACGGTGGCCTGGA7740.10875307712194958No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACGGTGGC7640.1073479986061621No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA7610.10692647505142587No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7560.10622393579353213No Hit
GGACCAGACTGACCGTCGTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG7500.10538088868405966No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7430.10439733372300845No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7400.10397581016827219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1253.3469405E-1045.6263670
GTATCAA22350.034.003881
TATCAAC23600.031.7559152
ATCAACG23650.031.6865483
ATGGCAA5800.031.68497370
TCAACGC23700.031.6174774
TGCGTTA553.588126E-430.8479568
TGACAGT8650.030.76922270
CAACGCA24600.030.6028735
AACGCAG24750.030.4280916
AATGGTA8050.029.91376370
CGCAGAG26100.028.9923528
ACGCAGA26100.028.862347
ATACGTA3550.028.68564811
AATACGT3550.028.68564810
TACGTAC3550.028.68564812
GTACGTA1353.2243406E-828.59494669
GTGCTAG3800.028.570931
ACGTACC3600.028.28723513
CGTACCA3650.027.89973814
Produced by FastQC (version 0.11.5)