FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416592
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGGAGCTCAGCCACTTACACTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAA69750.49237889244044863No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53510.37773755605001297No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48040.33912375616973695No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAATCTGTCCCAGACCCACTGCCA47090.3324175203587201No Hit
CTCCTGCAGGGCCAGTCAGACTGTAAACAACAACTATTTAGCCTGGTATC38980.2751674441194077No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38310.2704377830737432No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35940.25370748952415373No Hit
GTATGGGAGCTCAGCCACTTACACTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT34920.24650711002179881No Hit
GTCCAGGAGGGCCGCTGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCAGTCTCACCATCAG32400.22871793713362776No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31720.22391768413205776No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31460.22208229327851634No Hit
GTGTATTACTGTCAGCAGTATGGGAGCTCAGCCACTTACACTTTTGGCCA31320.22109400589584016No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31260.22067045416040754No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30730.21692908049741913No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29630.2091639653478207No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAATCTGTCCCAGACCCACTGCCA26260.18537447620768718No Hit
GCTCAGTGAGGACCCAGAGGGAACCATGGAAACCCCAGCGCACCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA25700.1814213266769825No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25200.17789172888171045No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACGCTCTCCTGCAGGGCCAGTCAGACTGTAAACAA24820.17520923455730372No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24620.17379739543919492No Hit
GTGAGGACCCAGAGGGAACCATGGAAACCCCAGCGCACCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA24450.17259733218880244No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23930.1689265504817195No Hit
CTCCTGGACGTAGCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTG23890.16864418265809775No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23800.16800885505494878No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23680.1671617515840835No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACGCTCTCCTGC23420.16532636073054202No Hit
CATCTATGCTACGTCCAGGAGGGCCGCTGGCACCCCAGACAGGTTCAGTG23320.16462044117148764No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22980.16222031467070266No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22780.16080847555259384No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22760.16066729164078294No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22440.15840834905180884No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCCTGGCCAAAAGTGTAAGTGGCTGAGCTCCCATACTG21990.15523171103606404No Hit
GTCTGGGACAGATTTCAGTCTCACCATCAGCAGACTGGAGCCTGAAGATT21130.1491608028281961No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20880.1473960039305601No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20460.1444311417825316No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18930.13363057252899918No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACGCTCTCCTGCAGGGCCAGTCAGA18480.13045393451325435No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC18440.1301715666896326No Hit
GTCTTTGACGGCCCCCTCAGAAATTACTACTGGAACTGGGTCCGGCAGCG18110.12784203214475304No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17680.1248065780408191No Hit
GTTATAGTCCGTCCCTCAGGAGTCGCCTCTTCTTATCAGTAGACACGTCC17650.12459480217310277No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17590.12417125043767013No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17510.1236065147904266No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCAGCGGCCCTCCT17390.1227594113195613No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17380.12268881936365589No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17300.12212408371641235No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT17270.12191230784869603No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17090.1206416526423981No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16470.11626495137626078No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGATACCAGGCTAAATAGTTGTTGTTTACAGTCTGA16390.11570021572901724No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16340.11534725594949005No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16150.11400600878728667No Hit
CCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCAGTCTCACC15880.11210002597783979No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15740.11111173859516361No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15730.11104114663925817No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15600.11012345121248744No Hit
CCCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGC15430.10892338796209494No Hit
CTCCCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAATCTGTC15360.10842924427075687No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15300.10800569253532422No Hit
CCTTGGTCCCCTGGCCAAAAGTGTAAGTGGCTGAGCTCCCATACTGCTGA15160.10701740515264804No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14990.10581734190225556No Hit
CCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCACCGGAGAAACTGTGTTGACGCAGTCTCCAGGCACC14880.1050408303872957No Hit
CTGCAGGAGAGCGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG14730.1039819510487141No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14560.1027818877983216No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14440.10193478432745631No Hit
TCTATGCTACGTCCAGGAGGGCCGCTGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCAGT14400.10165241650383457No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14300.10094649694478014No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14280.10080531303296926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)