FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416592
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGGAGCTCAGCCACTTACACTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAA65420.46181257553339283No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53110.37491387781379537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48460.34208861831776544No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAATCTGTCCCAGACCCACTGCCA44470.31392242791149466No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40770.2878034042264816No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38470.27156725436823026No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37640.26570812202807864No Hit
CTCCTGCAGGGCCAGTCAGACTGTAAACAACAACTATTTAGCCTGGTATC36340.2565311677603714No Hit
GTATGGGAGCTCAGCCACTTACACTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT33340.23535358098873918No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32620.23027096016354745No Hit
GTCCAGGAGGGCCGCTGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCAGTCTCACCATCAG32460.2291414888690604No Hit
GTGTATTACTGTCAGCAGTATGGGAGCTCAGCCACTTACACTTTTGGCCA31330.22116459785174558No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30890.2180585517919062No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28460.20090470650688413No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28300.1997752352123971No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27420.1935631430927183No Hit
GCTCAGTGAGGACCCAGAGGGAACCATGGAAACCCCAGCGCACCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA25160.17760936105808872No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25110.17725640127856152No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAATCTGTCCCAGACCCACTGCCA25040.17676225758722341No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACGCTCTCCTGCAGGGCCAGTCAGACTGTAAACAA24630.17386798739510034No Hit
GTGAGGACCCAGAGGGAACCATGGAAACCCCAGCGCACCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA24560.17337384370376227No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24110.17019720568801744No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23970.16920891830534127No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23080.16292623422975705No Hit
CTCCTGGACGTAGCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTG22530.1590436766549578No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCCTGGCCAAAAGTGTAAGTGGCTGAGCTCCCATACTG22480.15869071687543063No Hit
CATCTATGCTACGTCCAGGAGGGCCGCTGGCACCCCAGACAGGTTCAGTG22220.15685532602188915No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACGCTCTCCTGC22060.1557258547274021No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21710.1532551362707117No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21580.15233744084394096No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21210.14972553847543965No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21000.14824310740142538No Hit
GTCTGGGACAGATTTCAGTCTCACCATCAGCAGACTGGAGCCTGAAGATT20860.14725482001874923No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20530.14492528547386968No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19990.14111331985497588No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19720.13920733704552898No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACGCTCTCCTGCAGGGCCAGTCAGA19690.13899556117781267No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19640.13864260139828546No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18540.13087748624868698No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18270.1289715034392401No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCAGCGGCCCTCCT17600.12424184239357558No Hit
GTTATAGTCCGTCCCTCAGGAGTCGCCTCTTCTTATCAGTAGACACGTCC16960.11972395721562737No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16750.11824152614161312No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16650.1175356065825587No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC16430.115982583552639No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16390.11570021572901724No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15900.11224120988965065No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15830.11174706619831257No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15820.11167647424240713No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15510.10948812360933846No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15260.10772332471170244No Hit
CCTTGGTCCCCTGGCCAAAAGTGTAAGTGGCTGAGCTCCCATACTGCTGA15110.10666444537312085No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGATACCAGGCTAAATAGTTGTTGTTTACAGTCTGA15060.10631148559359364No Hit
GTCTTTGACGGCCCCCTCAGAAATTACTACTGGAACTGGGTCCGGCAGCG14850.10482905451957938No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14730.1039819510487141No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14670.10355839931328145No Hit
CCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCACCGGAGAAACTGTGTTGACGCAGTCTCCAGGCACC14610.10313484757784881No Hit
CCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCAGTCTCACC14520.10249951997469983No Hit
CTCCCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAATCTGTC14410.10172300845973999No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14370.10144064063611824No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14220.10038176129753662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1107.1860013E-637.79023770
GTATCAA41650.037.69071
GTGGTAT4400.036.514461
TATCAAC48150.032.382672
ATCAACG49150.031.5865463
TCAACGC49650.031.2684544
CAACGCA49750.031.2078065
AACGCAG50650.030.724766
AATGGTA7500.029.56036670
AGAGTAC52700.029.48169511
CGCAGAG52850.029.4540928
ACGCAGA53300.029.2023247
GCAGAGT53350.029.0535779
AGTTGAG8200.028.72672770
ATGGCAA4900.028.2784170
CAGTACT855.6215467E-627.5541364
CGTACAC500.008982968526.7668743
CGTGCAA1107.6261676E-726.30884657
CGGGTAT1157.520248E-826.21089417
CAGAGTA60500.025.62359410
Produced by FastQC (version 0.11.5)