FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614892
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAAGCTGTTGACAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC73110.45272377347835024No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC72610.449627591194953No Hit
GCTTAGTACTTACCCTCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAGGAACTGTGGCTGCACCAT71550.44306368475415076No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63490.39315322634578653No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC60690.37581460555876184No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51230.3172348367568853No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA48460.30008198690686433No Hit
GTACTTACCCTCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAGGA47990.2971715755604709No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46690.289121501623638No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42530.2633612650257726No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42390.26249433398642136No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG41610.25766428962432164No Hit
GGTAAGTACTAAGCTGTTGACAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATT41110.25456810734092433No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC38310.2372294865538996No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37970.23512408260118944No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36870.22831248157771541No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGG36270.2245970628376387No Hit
GATTAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGAAACCAGGCTAAAT35930.22249165888492853No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35840.22193434607391702No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35640.2206958731605581No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34780.21537043963311478No Hit
GCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCCTGCTGCTCTGG32530.20143761935782703No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTC32520.2013756957121591No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT31370.19425447646034533No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30660.1898578976179212No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30590.18942443209824558No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGAAGTCATTTAGCCTGGTTTCAGCAAAAA30570.18930058480690967No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG30300.18762864637387516No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30200.1870094099171957No Hit
GTCTCCACCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT30200.1870094099171957No Hit
CAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGAAAT28000.17338620787024767No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27660.1712808039175375No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27470.17010425464984655No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATATCCACTTTGGTCC27130.1679988506971364No Hit
GGACACAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCCTGCT27070.16762730882312873No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26300.16285918810669692No Hit
GTGCAGCCACAGTTCCTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG24920.15431372500452042No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24790.15350871761083712No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24760.1533229466738333No Hit
GTCTCCTACAGATGCAGACAGGAAGGGTGGAGACTGGGTCAACTGGATTTCACATCTGGCACCTGGGAGCCAGAG24260.15022676439043603No Hit
GGACATTAGAAGTCATTTAGCCTGGTTTCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTAATCTTTGCTGCATC23710.14682096387869903No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23660.14651134565035928No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23360.1446536362803209No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23060.14279592691028253No Hit
CTCCTAATCTTTGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAACGTT22970.14223861409927105No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22960.1421766904536031No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22750.14087629389457623No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22750.14087629389457623No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCAACAGCTTAGTACTTAC22580.13982359191822116No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22570.1397616682725532No Hit
AGTCAGGACATTAGAAGTCATTTAGCCTGGTTTCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTAATCTTTG22060.136603562343488No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21840.13524124213879318No Hit
ACTTATTACTGTCAACAGCTTAGTACTTACCCTCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAGGAA21650.13406469287110223No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21530.13332160912308688No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21450.13282621995774332No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21430.13270237266640741No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21400.13251660172940358No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21010.1301015795483537No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20990.1299777322570178No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20750.1284915647609871No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCC20570.12737693913896408No Hit
GTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG20490.12688154997362053No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20260.12545730612325778No Hit
CTAATGTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA20230.12527153518625395No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20220.125209611540586No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20120.12459037508390654No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19950.12353767310755147No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGAAATCCAGTTGACCCAGTCTCCACCCTTC19460.12050341446982213No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19140.11852185780844787No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19090.11821223958010814No Hit
GCTTAGGGGCTTTCCCTGGTTTTTGCTGAAACCAGGCTAAATGACTTCTA18960.11740723218642485No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18880.11691184302108129No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18840.11666414843840951No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18520.11468259177703526No Hit
CAATAATAGAGCGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGGAGATA18020.11158640949363796No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17720.1097287001235996No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17330.10731367794254972No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17320.10725175429688177No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17230.10669444148587025No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17220.1066325178402023No Hit
GTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGAAGGGTGGAGACTGGGTCAA17160.10626097596619463No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16780.10390787743081271No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16740.10366018284814092No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAAGGAACTGTGGCTGCACCA16660.10316479368279736No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16540.10242170993478202No Hit
CATTAGAAGTCATTTAGCCTGGTTTCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTAATCTTTGCTGCATCCA16260.10068784785607955No Hit
GGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC16180.10019245869073597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTT200.00749419651.51319540
CGTATGC4750.047.8625941
TATCTCG4550.047.75827436
TCGTATG4900.046.95760340
GGTATCA8450.046.925871
ATGCCGT5000.045.19280644
ACGGACT5050.045.03423327
CGAGACG5100.044.46972323
CTCCTAT5100.043.60505732
CACGAGA5150.043.3782621
GCCGTCT5250.043.3220646
TTATACA5350.042.9751472
ACGAGAC5300.042.7849922
TGCCGTC5300.042.77982745
ACTCCTA5200.042.6956431
TATACAC5400.042.577233
CGGACTC5350.042.54332728
TCCTATC5000.042.5260433
TATGCCG5400.042.35760543
CTTATAC5500.041.80311
Produced by FastQC (version 0.11.5)