FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614892
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGTACTTACCCTCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAGGAACTGTGGCTGCACCAT70270.43513745810865373No Hit
GTACTAAGCTGTTGACAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC70180.43458014529764216No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63600.393834386448134No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63130.39092397510174054No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC57760.35767097737805376No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55460.3434285388744263No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53720.33265382452820375No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC49040.3036735583556052No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45990.2847868464268818No Hit
GTACTTACCCTCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAGGA45100.27927564196243465No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40190.24887113193947336No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT39560.24496994226239277No Hit
GGTAAGTACTAAGCTGTTGACAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATT38310.2372294865538996No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36860.22825055793204746No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36330.22496860471164634No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT35850.22199626971958494No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGG35650.22075779680622606No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34920.216237370672466No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC34530.21382234849141615No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33920.21004500610567145No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG33050.20465764893256017No Hit
GATTAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGAAACCAGGCTAAAT32850.2034191760192013No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32060.1985272080114336No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31610.19574064395637605No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31540.1953071784367004No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGAAGTCATTTAGCCTGGTTTCAGCAAAAA30560.18923866116124174No Hit
GTCTCCACCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT30520.18899096657856995No Hit
GCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCCTGCTGCTCTGG30120.18651402075185214No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTC30010.18583286064950474No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29680.18378938034246253No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29330.18162205274408444No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27110.1678750034058005No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27090.16775115611446462No Hit
GGACACAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCCTGCT26210.1623018752956854No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26080.16149686790200213No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATATCCACTTTGGTCC26040.16124917331933034No Hit
CAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGAAAT25660.1588960747839484No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25130.1556141215635473No Hit
GTCTCCTACAGATGCAGACAGGAAGGGTGGAGACTGGGTCAACTGGATTTCACATCTGGCACCTGGGAGCCAGAG24860.15394218313051275No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24800.15357064125650507No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24560.1520844737604744No Hit
GTGCAGCCACAGTTCCTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG24300.1504744589731078No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24070.14905021512274505No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23200.14366285794963377No Hit
GGACATTAGAAGTCATTTAGCCTGGTTTCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTAATCTTTGCTGCATC23110.14310554513862228No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22950.14211476680793514No Hit
CTCCTAATCTTTGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAACGTT22920.1419289958709313No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC22560.13969974462688528No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21750.13468392932778167No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21410.13257852537507153No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCAACAGCTTAGTACTTAC21300.1318973652727241No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21130.13084466329636904No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21020.13016350319402165No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20870.12923464850900246No Hit
AGTCAGGACATTAGAAGTCATTTAGCCTGGTTTCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTAATCTTTG20630.12774848101297176No Hit
ACTTATTACTGTCAACAGCTTAGTACTTACCCTCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAGGAA20560.12731501549329616No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20400.12632423716260902No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20170.12489999331224627No Hit
GTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG20120.12459037508390654No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20050.12415690956423092No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCC19810.12267074206820024No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT19740.12223727654852462No Hit
CTAATGTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA19720.12211342925718871No Hit
GTGGGGTCCCATCAACGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT19490.12068918540682598No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19460.12050341446982213No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19410.1201937962414824No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGAAATCCAGTTGACCCAGTCTCCACCCTTC19400.12013187259581445No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19070.11808839228877226No Hit
GTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGAAGGGTGGAGACTGGGTCAA18770.1162306829187339No Hit
GCTTAGGGGCTTTCCCTGGTTTTTGCTGAAACCAGGCTAAATGACTTCTA18690.11573529375339031No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18160.11245334053298921No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17750.10991447106060344No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17630.10917138731258809No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17600.10898561637558427No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17160.10626097596619463No Hit
GTCCCAGATCCACTGCCGCTGAACGTTGATGGGACCCCACTTTGCAAAGT17060.10564173950951519No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16760.10378403013947682No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAAGGAACTGTGGCTGCACCA16710.1034744119111371No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16540.10242170993478202No Hit
CAATAATAGAGCGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGGAGATA16520.10229786264344613No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16330.10112131337575517No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16160.10006861139940008No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT150.002732816666.463544
TCGTACC352.8356491E-547.47692
GTCGTAC307.5819663E-444.3200041
GTGGTAT3400.040.083531
TGGTATC3500.037.981522
GTATCAA21600.036.4716721
GGTATCA9100.036.1622051
CGTACCC502.3198634E-433.231773
TATCAAC24200.032.135192
TCAACGC24500.031.6040924
CAACGCA24750.031.5573085
AACGCAG25050.031.3188426
CGCAGAG26100.029.9417238
AGAGTAC25900.029.9263411
TCCGAGA8650.029.86066870
ACGCAGA26300.029.8367757
CAGAGTA26100.029.6951810
GATTAAT450.005545659529.546671
TAAGGTC450.00554901829.5430095
CGTATCG500.00582987129.2458657
Produced by FastQC (version 0.11.5)