FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462202
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGTGGCCCCTACTCACTTTCGGCCCTGGGACCAAACTGGATATGAAACGAACTGTGGCTGCACCAT83450.570714579791301No Hit
CTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAAGT80990.5538906389130914No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA72970.49904185604998486No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC70760.4839276652610241No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70220.48023460506824645No Hit
GTCCCAGACCCAGCGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT59150.40452687111630264No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56940.3894126803273419No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50040.34222357786407076No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT48100.32895591717149886No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGAC46260.3163721565146266No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45630.3120635862897192No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44990.3076866260612419No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44870.3068659460184024No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG41370.282929444768917No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG40630.2778685845047401No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT40560.2773898544797504No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC40080.2741071343083924No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTTCA39930.273081284254843No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39860.27260255422985336No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38980.266584233915697No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTGGTAACCTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT38240.2615233736515201No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36650.2506493630838968No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36500.24962351303034735No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTTCACT36100.24688791288754902No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGA35320.2415534926090923No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35220.24086959257339272No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTGGTAACCTAGTCTGGTA34250.2342357622271068No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33040.22596057179514184No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT32940.22527667175944224No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31880.21802733138102667No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31730.21700148132747732No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGCGCCA31630.21631758129177775No Hit
GTGCAGCCACAGTTCGTTTCATATCCAGTTTGGTCCCAGGGCCGAAAGTGAGTAGGGGCCACTTATTATACTG31610.21618080128463785No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31430.21494978122037858No Hit
GAGTGAAGTCTGTCCCAGACCCAGCGCCACTGAACCTGGCTGGGATACCA30810.21070960099904118No Hit
GTGTTAGTGGTAACCTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCA30000.20517001070987456No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG29370.20086144048496718No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATATCCAGTTTGGTCCCAGGGCCGAAAGTGAGTAGGGGCCA29090.19894652038500837No Hit
GAGTGTTAGTGGTAACCTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC28390.19415922013511128No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27090.18526851967101673No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26720.18273808953892826No Hit
GTTTATTACTGTCAGCAGTATAATAAGTGGCCCCTACTCACTTTCGGCCC26070.17829273930688097No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC25970.1776088392711814No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAAGTGGCCCCTACTCA25900.1771301092461917No Hit
GTCAGAGTGTTAGTGGTAACCTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG25240.17261636901057448No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25140.17193246897487488No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24970.1707698389141856No Hit
CCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGCACCATAGAT24830.16981237886420616No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24490.1674871187428276No Hit
GACTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAAGTGG23610.16146879842867126No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23320.15948548832514248No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT23240.1589383682965828No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGG23100.1579809082466034No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22570.15435623805739562No Hit
CCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC21990.15038961785033805No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21800.14909020778250884No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21590.14765401770753972No Hit
CTTTCGGCCCTGGGACCAAACTGGATATGAAACGAACTGTGGCTGCACCA21580.14758562770396977No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21240.1452603675825912No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTC21240.1452603675825912No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21200.14498680756831137No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21070.14409773752190191No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGCGCCA21060.14402934751833193No Hit
GGTTTCTGCTGGTACCAGACTAGGTTACCACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC20850.14259315744336282No Hit
CCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCAC20730.14177247740052332No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20710.14163569739338341No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGACTAGGTTACCACTA20620.14102018736125377No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20370.13931043727200482No Hit
GTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGCGCCACTGAACCTGGC20340.13910526726129496No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20270.13862653723630525No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20210.13821619721488548No Hit
GTTCAGTGGCGCTGGGTCTGGGACAGACTTCACTCTCACCATCACCAGCC20200.13814780721131553No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20080.13732712716847603No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19970.1365748371292065No Hit
CCACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGGAGA19820.13554898707565713No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACC19520.13349728696855837No Hit
CACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTTCACTCTCACCATCACCAG19370.132471436915009No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19280.1318559268828794No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19220.13144558686145966No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19210.13137719685788968No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19150.1309668568364699No Hit
GCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGACTAGGTTACCA18960.12966744676864073No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18940.1295306667615008No Hit
CTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGA18940.1295306667615008No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18710.12795769667939177No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC18640.12747896665440206No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC18590.12713701663655227No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTGGTAACCTAGT18570.12700023662941237No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18540.12679506661870246No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT18010.12317039642949469No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17930.12262327640093502No Hit
TCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTTCACT17890.12234971638665519No Hit
CACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACA17610.12043479628669636No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17320.11845148618316757No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTGGTAACCTAGTCTGGTACCAGCAGAAACCTGGCCAGG17310.11838309617959762No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17290.1182463161724577No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17100.1169469061046285No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTT16970.11605783605821904No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA16780.11475842599038984No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16580.11339062591899068No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16580.11339062591899068No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16550.11318545590828079No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16540.11311706590471085No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16320.11161248582617175No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACG16170.11058663577262239No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16030.10962917572264297No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15810.1081245956441039No Hit
GACACAGACAGGGTGGCTGGAGACTGCGTCATCACTATTTCTCCAGTGGT15710.1074406956084043No Hit
GTACCAGACTAGGTTACCACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGA15240.10422636544061628No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15140.10354246540491668No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT15060.10299534537635702No Hit
ACTCACTTTCGGCCCTGGGACCAAACTGGATATGAAACGAACTGTGGCTG14930.10210627532994757No Hit
CCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTC14890.10183271531566773No Hit
GTGGTAACCTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGG14850.1015591553013879No Hit
GTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATC14840.10149076529781796No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14830.101422375294248No Hit
GAGTAGGGGCCACTTATTATACTGCTGACAGTAATAAACTGCAAAATCTT14800.10121720528353813No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTT14640.1001229652264188No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT502.282874E-456.52470
CCGACTA352.6325944E-548.07711814
CTTATAC4000.043.745691
CGTGCGT658.327853E-443.47999670
TCGTATG3250.043.3619840
TATACAC4300.041.4748153
CGTATGC3250.041.31459841
CTCTTAT1250.040.3806341
CGAGACG4300.039.9343123
TTATACA4500.038.8837282
CGCTCAT4650.038.50713328
ATGCCGT3600.038.50078644
AATGGTA6100.038.22319870
ACGAGAC4500.038.15162322
GTACGTA601.0559105E-437.9906169
CCGTCTT3900.037.5861747
GCCGTCT3650.037.2193446
CATGAAT4650.037.19323332
TACACAT4900.037.082995
TGTACCG400.001956667336.54786361
Produced by FastQC (version 0.11.5)