FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462202
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGTGGCCCCTACTCACTTTCGGCCCTGGGACCAAACTGGATATGAAACGAACTGTGGCTGCACCAT78920.53973390817411No Hit
CTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAAGT74800.5115572267032872No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA72100.49309192573939853No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69180.4731220446969707No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60310.4124601115304178No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54680.3739565395205314No Hit
GTCCCAGACCCAGCGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT54280.37122093937773304No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52200.35699581863518176No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT50120.3427706978926304No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46440.3176031765788858No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43700.2988643156007173No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGAC42750.29236726526157125No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42140.28819547504380383No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41360.28286105476534706No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTGGTAACCTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT39040.26699457393711673No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT38990.266652623919267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38110.26063430360511064No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37400.2557786133516436No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTTCA36970.2528378431981354No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC36950.2527010631909955No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36490.24955512302677743No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36250.24791376294109846No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTTCACT35510.24285290267692153No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35430.24230578264836186No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTGGTAACCTAGTCTGGTA34890.2386127224555841No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34160.23362025219497715No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33480.22896973195222No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33350.22808066190581053No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT33100.22637091181656158No Hit
GTGTTAGTGGTAACCTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCA33000.22568701178086203No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT32480.22213073159522417No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32250.22055776151311515No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGA31780.21734343134532713No Hit
GAGTGAAGTCTGTCCCAGACCCAGCGCCACTGAACCTGGCTGGGATACCA31320.21419749118110903No Hit
GTGCAGCCACAGTTCGTTTCATATCCAGTTTGGTCCCAGGGCCGAAAGTGAGTAGGGGCCACTTATTATACTG31280.2139239311668292No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGCGCCA30520.20872629089551237No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG29280.2002459304528376No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29240.19997237043855773No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28750.19662126026362978No Hit
GAGTGTTAGTGGTAACCTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC28640.19586897022436023No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28090.19210752002801257No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATATCCAGTTTGGTCCCAGGGCCGAAAGTGAGTAGGGGCCA27910.1908764999637533No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC27610.18882479985665454No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27430.1875937797923953No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAAGTGGCCCCTACTCA25040.1712485689391753No Hit
GTTTATTACTGTCAGCAGTATAATAAGTGGCCCCTACTCACTTTCGGCCC24940.1705646689034757No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24600.16823940878209714No Hit
GTCAGAGTGTTAGTGGTAACCTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG24520.16769228875353748No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24420.16700838871783788No Hit
GACTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAAGTGG24160.16523024862501898No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24160.16523024862501898No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23930.16365727854290993No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22280.15237292795386684No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGACTAGGTTACCACTA22120.1512786878967475No Hit
CCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGCACCATAGAT22080.15100512788246767No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22020.1505947878610479No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGG21740.1486798677610891No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21650.14806435772895948No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21400.14635460763971053No Hit
GGTTTCTGCTGGTACCAGACTAGGTTACCACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC21290.14560231760044096No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21290.14560231760044096No Hit
GTTCAGTGGCGCTGGGTCTGGGACAGACTTCACTCTCACCATCACCAGCC20770.14204603741480315No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTC20730.14177247740052332No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACC20670.14136213737910358No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20640.1411569673683937No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGCGCCA20620.14102018736125377No Hit
CTTTCGGCCCTGGGACCAAACTGGATATGAAACGAACTGTGGCTGCACCA20310.13890009725058508No Hit
CCACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGGAGA20220.13828458721845546No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20210.13821619721488548No Hit
CCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC19960.13650644712563653No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19780.1352754270613773No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19730.1349334770435275No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19640.13431796701139787No Hit
GTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGCGCCACTGAACCTGGC19470.13315533695070858No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT19450.13301855694356868No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19370.132471436915009No Hit
CCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCAC19340.13226626690429913No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19080.13048812681148023No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC18640.12747896665440206No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18460.1262479465901428No Hit
GTGGTAACCTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGG18360.12556404655444323No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTGGTAACCTAGT18020.12323878643306464No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17960.1228284464116449No Hit
GCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGACTAGGTTACCA17860.12214454637594532No Hit
CTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGA17690.12098191631525602No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17560.12009284626884656No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17460.11940894623314699No Hit
CACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTTCACTCTCACCATCACCAG17420.11913538621886716No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17400.11899860621172724No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17320.11845148618316757No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17320.11845148618316757No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17100.1169469061046285No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17070.11674173609391864No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT17030.1164681760796388No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16680.11407452595469025No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTGGTAACCTAGTCTGGTACCAGCAGAAACCTGGCCAGG16610.11359579592970055No Hit
TCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTTCACT16490.11277511588686105No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA16370.11195443584402155No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16230.11099697579404214No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16180.11065502577619235No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16020.10956078571907303No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTT16020.10956078571907303No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15960.10915044569765327No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACG15920.10887688568337343No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15880.1086033256690936No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15840.10832976565481377No Hit
CACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACA15760.1077826456262541No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15640.1069619655834146No Hit
GGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGGAGACTGCGTC15470.10579933552272532No Hit
GTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATC15140.10354246540491668No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15070.10306373537992698No Hit
CCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTC14820.10135398529067803No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14700.10053330524783853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)