FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596648
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25820.43275096874539093No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT24640.4129738137059037No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20770.34811144929673776No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19710.3303455303629611No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18610.3119091993939475No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18380.3080543301913356No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18110.3035290489534868No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG15880.26615357798903205No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG15410.258276236574999No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15020.2517397192314396No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13350.22375001676030087No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13190.22106836861935344No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12840.21520226331103098No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12190.20430806773843205No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11490.19257585712178707No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11300.18939139995441198No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC10760.1803408374787144No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10110.1694466419061155No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10010.16777061181802336No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA9910.16609458172993122No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9870.16542416969469437No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9640.16156930049208243No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9540.1598932704039903No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCT9440.15821724031589815No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG8970.15033989890186508No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8910.1493342808490098No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8860.14849626580496375No Hit
GGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCAT8520.14279776350545045No Hit
GGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAA8460.14179214545259516No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8230.13793727624998323No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8220.13776967324117403No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACC8210.13760207023236481No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8150.13659645217950953No Hit
GAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTGTCTGTTGTCATGGCGGAGGTGGACGGCAC8020.13441761306498975No Hit
GGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCC7890.13223877395046996No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA7660.12838390474785802No Hit
GCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGT7550.1265402716509567No Hit
GCCCCAAGGACGTGCTGGTTCGCTGGCTGCAGGGGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCAT7550.1265402716509567No Hit
GTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCG7500.12570225660691062No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7480.12536705058929218No Hit
CCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTC7320.12268540244834475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7320.12268540244834475No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT7240.12134457837787103No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7240.12134457837787103No Hit
AGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTG7190.12050656333382496No Hit
GACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGC7130.11950094528096968No Hit
ATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGA6700.11229401590217349No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6650.1114560008581274No Hit
CCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAG6550.10977997077003528No Hit
GGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGGTGGTGCC6480.10860674970837075No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6400.10726592563789705No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6380.10693071962027863No Hit
TATTCATTCTCCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGG6340.10626030758504176No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6340.10626030758504176No Hit
GCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGC6270.10508708652337727No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG6250.10475188050575884No Hit
GTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTG6220.10424907147933118No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG6180.10357865944409435No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6180.10357865944409435No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA6100.10223783537362063No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6080.1019026293560022No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6070.10173502634719297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA150.002464976968.209938
GACCGGA606.014945E-446.4391570
GGTATCA13800.042.847771
CAGGTAT552.1576125E-741.809041
GTATCAA32750.039.620561
TCGTATG1050.039.42308840
TAGGCAA909.452397E-538.69929570
GCCGTCT1100.038.6203546
CAAGGGT350.001714138737.542814
TTAGTGC400.001735218637.4486458
CGTCTTC1150.037.26436248
CTCGTAT754.2626198E-836.55037739
CGTATGC1151.8189894E-1236.17329841
GTGGTAT2850.035.731531
CTCTTAT657.992039E-735.3768771
CCGTCTT1251.8189894E-1234.1153447
CACGAGA1350.034.09234221
ATCTCGT808.205825E-833.927837
TCGCCTA400.0029090933.70602835
TGAGCGA1500.033.330531
Produced by FastQC (version 0.11.5)