FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004686877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260043
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC58090.4610160129455899No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC53830.42720764291377356No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCAAAGACCACATGGTTATCG43690.3467341987535346No Hit
CTATTACTGTATCTCCCGGGACAGCAGCGATAACCATGTGGTCTTTGGCG43230.34308352968906614No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42490.33721071423753No Hit
CCTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT42410.33657581526979635No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG41320.3279253168344255No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCAAAGACCAC41080.3260206199312246No Hit
CAGTAATAGTCACCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGA40690.32292548746352306No Hit
CTCTTTACATAGGTTCTGTGCTTTCTTCTGAGCTGACTCAGGATCCTGCT37740.29951358802834505No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37190.2951486576251763No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTTCTGAGGCTGTCTCCTTGGCATGTGATCTTGACTGT35940.28522836125433815No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG32960.26157837470626No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC32450.2575308937869581No Hit
CCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTATATTTCCTGAACTGGAGTCAGAGAATCGGT32000.25395958709345634No Hit
AGCCAGGACAGGCCCCTTCACTTGTCATCTATGGTAAAGACAAACGGCCC31190.24753123504515323No Hit
GGATGAGGGTGACTATTACTGTATCTCCCGGGACAGCAGCGATAACCATG30720.24380120360971808No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29340.23284919641631277No Hit
CTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTT28030.2224527258196744No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26280.208564310900501No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCCGCAGCTCTGGTCTCC25810.20483427946506588No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25620.20332639441669847No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25330.20102488565866403No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23910.18975542898139192No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23430.18594603517499006No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23320.1850730490943563No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23280.18475559961048948No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22700.1801525820944206No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCCGCAG21740.1725337944816169No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21600.17142272128808303No Hit
GTCTGTGGCCTTGGGACAGACAGTCAAGATCACATGCCAAGGAGACAGCCTCAGAACCTATTATGCAAGCTGGTA21510.17070845994938266No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTACATAGGTTCTG21400.16983547386874892No Hit
GTCAAGATCACATGCCAAGGAGACAGCCTCAGAACCTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCC21050.16705779088491424No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20890.16578799294944696No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20500.16269286048174547No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19640.15586769657860883No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCCGCAG19200.1523757522560738No Hit
ATCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAAGATCACATGCCAAGGAGACAGCCTCAGAACCTATTATG18330.14547122598197046No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA18320.14539186361100376No Hit
GCATAATAGGTTCTGAGGCTGTCTCCTTGGCATGTGATCTTGACTGTCTG18310.14531250124003706No Hit
GGACAGGCCCCTTCACTTGTCATCTATGGTAAAGACAAACGGCCCTCAGG18250.14483632701423682No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18180.14428079041746988No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18020.1430109924820026No Hit
CCTTGGTCCCTCCGCCAAAGACCACATGGTTATCGCTGCTGTCCCGGGAGATACAGTAATAGTCACCCTCATCCT18010.1429316301110359No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17870.141820556917502No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCAAAGACCACATGGTTATCGC17840.1415824698046019No Hit
TCACTGGGGCTCAGGCGGAGGATGAGGGTGACTATTACTGTATCTCCCGG17740.14078884609493486No Hit
CTTCTGAGCTGACTCAGGATCCTGCTGTGTCTGTGGCCTTGGGACAGACA17660.14015394712720122No Hit
CTCCTCACTCTTTACATAGGTTCTGTGCTTTCTTCTGAGCTGACTCAGGATCCTGCTGTGTCTGTGGCCTTGGGA17400.13809052548206688No Hit
GTGAAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTT16890.13404304456276492No Hit
GTAATAGTCACCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG16880.13396368219179822No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16750.13293197136923104No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTTCACTTGTCATCTATGGTAAAGACAAACGGCC16600.13174153580473047No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16570.13150344869183034No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT16420.1303130131273298No Hit
GGCTCACTCTCCTCACTCTTTACATAGGTTCTGTGCTTTCTTCTGAGCTGACTCAGGATCCTGCTGTGTCTGTGG15970.12674170643382804No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC15940.12650361932092793No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15780.12523382138546066No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG15770.12515445901449396No Hit
GCCTTGGGACAGACAGTCAAGATCACATGCCAAGGAGACAGCCTCAGAAC15280.1212657028371254No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTACATAGGTTCTGTGCTTTCTTCTGAG15220.12078952861132516No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15170.12039271675649164No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15120.11999590490165812No Hit
GGATCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAAGATCACATGCC15020.11920228119199107No Hit
GGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTTCTGAGGCTGTCTCCTTGGCATGTGATCTTGA14700.11666268532105649No Hit
TCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTATATTTCCTGAACTGGAGTCAGAGAATCGGTCTGGGATC14620.11602778635332285No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14570.11563097449848933No Hit
GTCCCGGGAGATACAGTAATAGTCACCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTATATTTC14570.11563097449848933No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14290.1134088281114216No Hit
GTGATGGTCAAGGAAGCTATATTTCCTGAACTGGAGTCAGAGAATCGGTC13990.11102795698242043No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCAAAGACCACATGGTTAT13810.10959943430501976No Hit
GGGACAGACAGTCAAGATCACATGCCAAGGAGACAGCCTCAGAACCTATT13750.10912326007921952No Hit
GCCCTCAGGGATCCCAGACCGATTCTCTGACTCCAGTTCAGGAAATATAG13710.1088058105953527No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13600.10793282451471894No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13540.10745665028891872No Hit
CTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13520.1072979255469853No Hit
GTTCTGAGGCTGTCTCCTTGGCATGTGATCTTGACTGTCTGTCCCAAGGC13320.1057106781276512No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13300.10555195338571778No Hit
GATCTTGACTGTCTGTCCCAAGGCCACAGACACAGCAGGATCCTGAGTCA13290.10547259101475108No Hit
GACTATTACTGTATCTCCCGGGACAGCAGCGATAACCATGTGGTCTTTGG13260.10523450390185096No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13200.10475832967605074No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13160.10444088019218392No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12970.10293299514381653No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12820.10174255957931594No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12750.101187022982549No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)