FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260043
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC57070.45292105110698605No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC54690.43403280681691025No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCAAAGACCAC44200.3507816796728366No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42300.3357028291891626No Hit
CTATTACTGTATCTCCCGGGACAGCAGCGATAACCATGTGGTCTTTGGCG41320.3279253168344255No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39650.31467180088298574No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCAAAGACCACATGGTTATCG39500.31348136531848514No Hit
CCTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT38270.3037197936895804No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38000.3015770096734794No Hit
CTCTTTACATAGGTTCTGTGCTTTCTTCTGAGCTGACTCAGGATCCTGCT37340.2963390931896769No Hit
CAGTAATAGTCACCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGA36680.2911011767058743No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTTCTGAGGCTGTCTCCTTGGCATGTGATCTTGACTGT36420.28903775506074No Hit
CCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTATATTTCCTGAACTGGAGTCAGAGAATCGGT31480.24983274380318768No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31220.24776932215805333No Hit
GGATGAGGGTGACTATTACTGTATCTCCCGGGACAGCAGCGATAACCATG31140.2471344231903197No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG30470.24181714433555043No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC30310.24054734640008316No Hit
CTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTT28760.22824617890024387No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28160.22348443664224155No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27780.2204686665455068No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26360.20919920986823465No Hit
AGCCAGGACAGGCCCCTTCACTTGTCATCTATGGTAAAGACAAACGGCCC24480.19427908412649408No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23810.18896180527172485No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23620.18745392022335747No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCCGCAGCTCTGGTCTCC23500.186501571771757No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCCGCAG23270.18467623723952278No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22300.17697808725575237No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21930.17404167952998428No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTACATAGGTTCTG21870.17356550530418408No Hit
GTCTGTGGCCTTGGGACAGACAGTCAAGATCACATGCCAAGGAGACAGCCTCAGAACCTATTATGCAAGCTGGTA21500.17062909757841596No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21410.16991483623971562No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20690.16420074553011285No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20570.1632483970785124No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20130.15975645275597739No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19520.15491534812700836No Hit
GTCAAGATCACATGCCAAGGAGACAGCCTCAGAACCTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCC19490.15467726101410825No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19420.15412172441734132No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19370.15372491256250778No Hit
CCTTGGTCCCTCCGCCAAAGACCACATGGTTATCGCTGCTGTCCCGGGAGATACAGTAATAGTCACCCTCATCCT18410.1461061249497041No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCCGCAG18390.1459474002077707No Hit
CTCCTCACTCTTTACATAGGTTCTGTGCTTTCTTCTGAGCTGACTCAGGATCCTGCTGTGTCTGTGGCCTTGGGA17970.14261418062716907No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17750.14086820846590156No Hit
GGACAGGCCCCTTCACTTGTCATCTATGGTAAAGACAAACGGCCCTCAGG17580.13951904815946758No Hit
ATCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAAGATCACATGCCAAGGAGACAGCCTCAGAACCTATTATG17550.13928096104656745No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17350.13769371362723337No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17200.13650327806273277No Hit
GGCTCACTCTCCTCACTCTTTACATAGGTTCTGTGCTTTCTTCTGAGCTGACTCAGGATCCTGCTGTGTCTGTGG17150.13610646620789923No Hit
GCATAATAGGTTCTGAGGCTGTCTCCTTGGCATGTGATCTTGACTGTCTG17000.1349160306433987No Hit
CTTCTGAGCTGACTCAGGATCCTGCTGTGTCTGTGGCCTTGGGACAGACA16900.13412240693373162No Hit
TCACTGGGGCTCAGGCGGAGGATGAGGGTGACTATTACTGTATCTCCCGG16270.12912257756282922No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTTCACTTGTCATCTATGGTAAAGACAAACGGCC16010.12705915591769487No Hit
GGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTTCTGAGGCTGTCTCCTTGGCATGTGATCTTGA15930.12642425694996123No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15910.1262655322080278No Hit
GTGAAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTT15740.12491637190159384No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCAAAGACCACATGGTTATCGC15580.12364657396612656No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG15510.12309103736935963No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC15330.12166251469195892No Hit
GTAATAGTCACCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG15170.12039271675649164No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTACATAGGTTCTGTGCTTTCTTCTGAG15110.11991654253069141No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15050.11944036830489119No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15000.11904355645005765No Hit
GTCCCGGGAGATACAGTAATAGTCACCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTATATTTC14970.11880546933715755No Hit
GGATCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAAGATCACATGCC14860.11793248325652379No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14480.11491671315978899No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14250.11309137862755478No Hit
GCCCTCAGGGATCCCAGACCGATTCTCTGACTCCAGTTCAGGAAATATAG14230.11293265388562138No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCAAAGACCACATGGTTAT14210.11277392914368796No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14150.11229775491788774No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14090.11182158069208749No Hit
GTGATGGTCAAGGAAGCTATATTTCCTGAACTGGAGTCAGAGAATCGGTC13960.11078986986952032No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13860.10999624615985328No Hit
GTATCAACGCAGAGTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCT13720.1088851729663194No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13640.10825027399858576No Hit
GCCTTGGGACAGACAGTCAAGATCACATGCCAAGGAGACAGCCTCAGAAC13510.10721856317601859No Hit
AGCCAGGACAGGCCCCTTCACTTGTCATCTATGGTAAAGACAAACGGCCCTCAGGGATCCCAGACCGATTCT13250.10515514153088426No Hit
TCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTATATTTCCTGAACTGGAGTCAGAGAATCGGTCTGGGATC13230.10499641678895086No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13010.10325044462768335No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACT13010.10325044462768335No Hit
CTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12910.1024568209180163No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12650.10039339927288196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2100.051.3461041
GGTATCA8300.042.2213441
GTATCAA21450.038.4871371
TGGTATC3100.034.773182
ATCAACG24800.032.5959743
CAACGCA25300.032.0887375
TCAACGC25200.032.078584
AACGCAG25700.031.5918126
ACGCAGA26350.030.6895267
CGCAGAG26750.030.2366188
AGAGTAC26550.030.2237211
CAGAGTA26850.030.0080210
GACGTTT450.00516788329.9756916
TATCAAC27800.029.203062
CCTATGA10750.028.2105051
CTATGAA11650.026.0239182
GAACACT11850.025.5867276
GAGTACG28400.025.52440512
AACACTC11800.025.413687
AGTACGG28700.025.25759913
Produced by FastQC (version 0.11.5)