FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348315
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62820.46591486410816463No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55240.4096965471718404No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46160.3423532334803069No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42660.31639490771815193No Hit
GAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTACTGATCTATAAGGCGT40640.30141324542113673No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38600.2862832498340521No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36760.2726365871476621No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35720.2649232560640503No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35040.25987992420168876No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34310.2544657591141536No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33490.24838409422130586No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31100.23065826605800574No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30550.22657910058109568No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTA30440.22576326748571365No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGAATAACTATGAAACTG29920.22190660194390777No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29800.22101660220349104No Hit
CCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCG28690.21278410460463618No Hit
TCTATAAGGCGTCTAATTTACAAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT28260.20959493886814284No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAA27910.20699910629192733No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27510.20403244049053817No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27010.20032410823880176No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26860.19921160856328085No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26300.19505827644133605No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26020.19298161038036366No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24920.1848232794265435No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24580.18230161349536272No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA23830.17673911511775808No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC23720.1759232820223761No Hit
TAGTTATTCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT22840.16939661725931995No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22510.1669491179731739No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCAACAGA21850.16205411940088185No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT21590.16012578662997892No Hit
GTTATTCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA21370.15849412043921488No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21230.15745578740872868No Hit
CCTTATAGATCAGTAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTACTAATACTCTGA20950.15537912134775628No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20330.15078078935560313No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19930.14781412355421397No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19880.1474432903290403No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19760.14655329058862357No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19680.14595995742834575No Hit
GAATAACTATGAAACTGCTGGCAGTAATAAGTTGCAGAATCATCAGGCTG18930.1403974590507411No Hit
GTTTCATAGTTATTCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT18700.13869162621494235No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18690.13861745956990762No Hit
GATCTATAAGGCGTCTAATTTACAAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA18510.1372824599592825No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18200.13498329396320594No Hit
ACTTATTACTGCCAGCAGTTTCATAGTTATTCTCCGTGGACGTTCGGCCA18140.13453829409299756No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18130.13446412744796282No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTATTACTGCCAGC18040.13379662764265027No Hit
GAGAATAACTATGAAACTGCTGGCAGTAATAAGTTGCAGAATCATCAGGC17770.1317941282267126No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17350.128679129135254No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17200.12756662945973307No Hit
CTATGAAACTGCTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC17140.1271216295895247No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC17080.12667662971931634No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16780.12445163036827447No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16590.12304246411261463No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16270.12066913147150334No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAA16250.12052079818143387No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16180.12000163166619075No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16160.11985329837612131No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTACTGATCTATAAGGCGTCTAATTTACAAACTGGGGTCCCAT15840.11747996573500999No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15810.1172574657999058No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15750.11681246592969745No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15680.11629329941445433No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15610.11577413289921124No Hit
CCCCTAAACTACTGATCTATAAGGCGTCTAATTTACAAACTGGGGTCCCA15380.1140683000634125No Hit
ATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA15350.11384580012830829No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15220.11288163374285683No Hit
GTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT15070.11176913406733589No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15050.11162080077726645No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15020.11139830084216226No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14920.11065663439181496No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14730.10924746813615513No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14700.10902496820105094No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14660.10872830162091203No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14630.10850580168580784No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTACTA14590.10820913510566893No Hit
ACCTTGATGGGACCCCAGTTTGTAAATTAGACGCCTTATAGATCAGTAGTTTAGGGGCTTTCCCTGGTTTCTG14590.10820913510566893No Hit
CTACTGATCTATAAGGCGTCTAATTTACAAACTGGGGTCCCATCAAGGTT14570.10806080181559947No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14480.10739330201028692No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14480.10739330201028692No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14350.10642913562483544No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14290.10598413575462708No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14230.1055391358844187No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14140.10487163607910614No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTCTG14040.10412996962875887No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13890.10301746995323793No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13760.10205330356778647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT853.6379788E-1271.8950870
TCGTATG2750.048.9191540
ATCTCGT2800.047.5542237
CTTATAC3600.045.332821
CTAGCGC3400.045.2790328
ACTAGCG3400.045.23351327
CGTATGC2850.044.90797441
TAGCGCT3450.044.6777629
GAGACTA3450.044.45052324
GACTAGC3500.043.88394526
CGCTCAT3550.043.691532
AGACTAG3550.043.23049525
GCCGTCT2850.041.9093146
ATGCCGT3100.041.66513444
GCGCTCA3900.041.40323331
GGTACGT901.0550139E-940.60177668
CGAGACT3800.040.3429223
TATACAC4300.040.2767143
ATCTCCG3900.040.1332310
TTATACA4200.039.6496962
Produced by FastQC (version 0.11.5)