FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241747
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC32780.26398292083653113No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT31960.2573793212304922No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30530.24586328777118044No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC30270.24376946350585105No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25670.20672488035002298No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG25420.20471158778720625No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG25150.20253723181936417No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23770.19142385687261576No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23090.1859477011017542No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22880.18425653534898817No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22810.18369281343139945No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22550.18159898916607006No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTACCACTGTCCCAAACCTGACAGTAATAGT22360.18006888681832933No Hit
GTCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGGTTAT21480.1729820969972144No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20570.16565371206856147No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20440.16460679993589677No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAGTTCCAATGTTGTTTCCCCCACAGATAA20250.16307669758815604No Hit
GTGGTAGTGATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA20220.16283510248061803No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19470.15679522479216781No Hit
GGGTTATCTGTGGGGGAAACAACATTGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC19050.15341289328663568No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCCTCAGGTATCCCT18840.15172172753386962No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTACCACTGTCCCAAACCTGA18840.15172172753386962No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG18660.15027215688864157No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18420.14833939602833748No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTACCAC18100.14576238154793206No Hit
GTGTATTACTGCGTGGTAGCTGCTAGCGTCGCGTTGTCAACTTTTGATAT17510.14101101109968456No Hit
GCCCTCAGGTATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG17490.14084994769465922No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA17490.14084994769465922No Hit
CCCCAGGACAGACGGCCAGGGTTATCTGTGGGGGAAACAACATTGGAACT16670.13424634808862032No Hit
CACTACCACTGTCCCAAACCTGACAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGTGG16670.13424634808862032No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT16390.13199146041826557No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16150.13005869955796148No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15680.12627370953986602No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15670.12619317783735334No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15560.12530732910971398No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15550.1252267974072013No Hit
GTCCCAAACCTGACAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGC15440.12434094867956194No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG15280.12305244143935923No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTAC15200.12240818781925787No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14800.11918691971875107No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14500.11677096864337098No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14400.11596565161824429No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14310.11524086629563027No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14110.11363023224537688No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG14030.11298597862527551No Hit
CAGGTATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC13960.11242225670768684No Hit
GGGATACCTGAGGGCCGGGCGCTATCATCATAGACGACCAGCACAGGGGC13890.11185853479009815No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13860.11161693968256015No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13760.11081162265743343No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13440.10823460817702801No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13340.10742929115190132No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13300.10710716434185065No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTGTGACCTCCTATGTGCTGACT13130.10573812539913525No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAGGCCGGGGATGAGGCCGACTAT12930.10412749134888187No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12930.10412749134888187No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12730.10251685729862846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)