FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241747
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38300.3084364206235248No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC36940.2974841090818017No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT31060.25013146800435193No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC29030.23378353239428No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA28080.22613302065557636No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24640.19843011499121802No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24060.19375927624548317No Hit
GTGGTAGTGATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA23870.19222917389774247No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23780.19150438857512844No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG23100.1860282328042669No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG23040.18554504258919088No Hit
GGGTTATCTGTGGGGGAAACAACATTGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC23010.18530344748165287No Hit
GTCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGGTTAT22820.18377334513391214No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21440.17265997018716372No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTACCACTGTCCCAAACCTGACAGTAATAGT21060.1695997654916823No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20950.1687139167640429No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20920.16847232165650491No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20160.16235191226554202No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19830.1596943660826239No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19720.15880851735498455No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18900.15220491774894565No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCCTCAGGTATCCCT18560.1494668398635149No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTACCACTGTCCCAAACCTGA17780.14318536706752666No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAGTTCCAATGTTGTTTCCCCCACAGATAA17720.14270217685245062No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17220.13867559172681712No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTACCAC16960.13658176746148773No Hit
GCCCTCAGGTATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG16700.13448794319615834No Hit
CCCCAGGACAGACGGCCAGGGTTATCTGTGGGGGAAACAACATTGGAACT16420.13223305552580356No Hit
GTGTATTACTGCGTGGTAGCTGCTAGCGTCGCGTTGTCAACTTTTGATAT16390.13199146041826557No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15290.1231329731418719No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA15200.12240818781925787No Hit
GTCCCAAACCTGACAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGC14950.12039489525644113No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTAC14840.11950904652880176No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14820.11934798312377642No Hit
CACTACCACTGTCCCAAACCTGACAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGTGG14730.1186231978011624No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG14680.11822053928859905No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTGTGACCTCCTATGTGCTGACT14350.11556299310568094No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14240.11467714437804158No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14100.1135497005428642No Hit
GGGATACCTGAGGGCCGGGCGCTATCATCATAGACGACCAGCACAGGGGC14050.11314704203030086No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13830.11137534457502213No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13320.10726822774687597No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13290.10702663263933795No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13180.10614078391169861No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13180.10614078391169861No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATACCTGAGGGCCGGGCGCTATCATCATAGA13080.10533546688657189No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13070.10525493518405923No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12900.10388589624134385No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12870.10364430113380585No Hit
CAGGTATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC12770.10283898410867913No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12620.10163100857098908No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12460.10034250133078637No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12430.10010090622324837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG150.00260491167.2711645
GCGTATA350.001521145538.47010415
GTATCAA35900.034.766891
ACCGGAC400.002724056634.16393336
ACTCCGC400.002930533.6586278
GGTATCA16250.033.124781
AACGCAG41450.030.1941176
ATCAACG42600.029.3683683
TCAACGC42950.029.1290474
TATCAAC43300.028.8970832
TCTCCGA500.00624123228.8400457
CAACGCA43450.028.797325
CGCAGAG43650.028.5307948
ACGCAGA43700.028.4935577
GAGTTTA6150.028.4455681
AGAGTAC44400.028.13146611
AGTTTAT6350.028.0737932
GATCGTT500.007144274728.05295448
CGTGCGT1256.3656236E-427.96957470
ATTTCGG1700.027.81977529
Produced by FastQC (version 0.11.5)