FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522315
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT50410.33114040129670924No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC47600.3126816723214315No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAG43470.28555193898766024No Hit
GTACAGGGGCCTGACCTGGCTTCTGCTGGTACCAGTTTGCATAATAGAAT42800.28115074738145523No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA42410.27858885973008213No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40540.266304936888883No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC39950.26242926069834427No Hit
CCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA39830.2616409875748449No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39360.2585535845078056No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG38260.25132774754239434No Hit
GACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAATGATTACCA34910.22932178951136922No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34560.22702265956782924No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32050.2105346134013No Hit
GTGTATTACTGTGCCGCGGGCGATTACTATGATAGTAGTACTTATTCCCACTTTGACTACTGGGGCCAGGGAACC32000.21020616626650857No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31810.20895806715430118No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATA30190.19831637998705917No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTAATCATTATGTCTTCGGAG29250.1921415738529805No Hit
ACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAATGATTACCAC28420.18668935141544293No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTAATCATTATGTCTTCGG27440.1802517875735311No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG26340.17302595060811987No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26240.17236905633853702No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25660.16855906957495656No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25440.1671139021818743No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCATATGCCAAGGAGACAGCCTCAGATT25440.1671139021818743No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24990.16415787796875153No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA24690.16218719516000304No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA24130.15850858725033912No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24090.158245829542506No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT23750.15601238902592435No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG22290.14642173269001488No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAATGATTAC21720.1426774353533927No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG21710.1426117459264344No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21670.14234898821860129No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA20900.1372909023428134No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20740.13623987151148087No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20380.13387505214098264No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19760.12980230766956904No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19390.12737179887211253No Hit
GGTCAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGG19010.12487560064769776No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18530.12172250815370013No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17940.11784683196316137No Hit
CTCCAGCCCCTTCCCCGGAGCCTGGCGGACCCAGGTCATCCAATAGCTAC17770.11673011170487055No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17700.11627028571616256No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA17660.11600752800832942No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCATATGCCAAGGAGACAGCCTCAGATTCTATTATGCAAACTGGTA17640.11587614915441284No Hit
CTATTATGCAAACTGGTACCAGCAGAAGCCAGGTCAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCC17560.11535063373874657No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17420.11443098176133061No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17320.11377408749174776No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17320.11377408749174776No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17190.11292012494129007No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17130.11252598837954038No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16760.11009547958208386No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16740.1099641007281673No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16670.10950427473945931No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGG16630.10924151703162617No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16630.10924151703162617No Hit
CTCCAGGGACAACGCCAAGAAGTCAGTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTA16590.10897875932379304No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16530.10858462276204332No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15700.10313240032450578No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15700.10313240032450578No Hit
GTATTACTGTGCCGCGGGCGATTACTATGATAGTAGTACTTATTCCCACTTTGACTACTGGGGCCAGGGAACC15660.10286964261667264No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15650.10280395318971434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC3850.049.4341
ACGAGAC3900.047.05891422
TCTCGTA2800.046.63379338
CACGAGA4050.045.31152321
GGTATCA8800.044.7995641
TATACAC4400.044.7995643
CGAGACG4100.044.76335523
CGCTCAT4200.044.58733428
GACGCTC4200.043.7406726
TTATACA4300.043.4703062
CGTATGC3100.043.4515241
ATGCCGT3050.043.24944344
TCGTATG2950.043.24859240
AATCTCG3100.043.10759436
CCCACGA4350.042.97205719
TACACAT4550.041.828775
AGACGCT4400.041.73187325
GAATCTC3200.041.72731835
ACACATC4650.040.9292266
GAGACGC4500.040.7884124
Produced by FastQC (version 0.11.5)