FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522315
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC50780.3335709100941658No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT46490.305390145929062No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC44930.29514259532356973No Hit
GTACAGGGGCCTGACCTGGCTTCTGCTGGTACCAGTTTGCATAATAGAAT40680.267224588866299No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAG40020.2628890866870523No Hit
CCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA39890.2620351241365946No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA38620.25369256691289255No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC36740.24134295464473518No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36070.23694176303853012No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34410.226037318163455No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34350.22564318160170532No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33770.2218331948381248No Hit
GTGTATTACTGTGCCGCGGGCGATTACTATGATAGTAGTACTTATTCCCACTTTGACTACTGGGGCCAGGGAACC33710.22143905827637514No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32490.21342494818746446No Hit
GACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAATGATTACCA31230.2051480803907207No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30040.19733103858268491No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATA29730.19529466634697812No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTAATCATTATGTCTTCGGAG27190.178609551899574No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA26810.1761133536751592No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26660.17512801227078495No Hit
ACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAATGATTACCAC26120.1715807832150376No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26120.1715807832150376No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTAATCATTATGTCTTCGG24890.1635009836991687No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24890.1635009836991687No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23620.15515842647546665No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCATATGCCAAGGAGACAGCCTCAGATT23590.15496135819459178No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT23530.1545672216328421No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22700.14911499919530452No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22650.14878655206051308No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22080.14504225472389093No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAATGATTAC21840.1434657084768921No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA21490.14116657853335216No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA21320.14004985827506133No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21220.1393929640054785No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20690.13591142437668946No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20460.13440056755664892No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG20280.13321815787139982No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19230.12632076804078No Hit
CTCCAGCCCCTTCCCCGGAGCCTGGCGGACCCAGGTCATCCAATAGCTAC19010.12487560064769776No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18960.12454715351290634No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18660.12257647070415781No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18530.12172250815370013No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCATATGCCAAGGAGACAGCCTCAGATTCTATTATGCAAACTGGTA18260.11994889362582646No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18180.11942337821016019No Hit
GGTCAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGG17660.11600752800832942No Hit
CTATTATGCAAACTGGTACCAGCAGAAGCCAGGTCAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCC17540.11521925488483002No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17350.11397115577262262No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16240.1066796293802531No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16190.10635118224546168No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16040.10536584084108742No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15870.1042491205827966No Hit
CTCCAGGGACAACGCCAAGAAGTCAGTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTA15790.10372360516713033No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15570.10227843777404808No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15500.1018186117853401No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA15490.1017529223583818No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15470.10162154350446524No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15420.10129309636967383No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGG15390.10109602808879897No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15390.10109602808879897No Hit
GGCCTGACCTGGCTTCTGCTGGTACCAGTTTGCATAATAGAATCTGAGGCTGTCTCCTTGGCATATGATCCTGA15370.1009646492348824No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15250.10017637611138298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)