FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987074
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39280.3979438218411183No Hit
GCCTTGGGCTGACCTACGACGGTCACCTTGGTACCTCCGCCGAGCACCACGAGAGTGTTGGTTGCATATGAGCTG34640.35093620133850145No Hit
CTCCTGGACAGTCCCTCACCATCTCCTGCACTGGAACCAGCAGTGACATT29470.298559175907784No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27350.2770815561953815No Hit
GTCCCTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATGACTTTGTCTCCTGGTACCGACACCT25910.262492984315259No Hit
GACCTACGACGGTCACCTTGGTACCTCCGCCGAGCACCACGAGAGTGTTGGTTGCATATGAGCTGCAATAATAAT25850.26188512715358725No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24210.2452703647345589No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24130.24445988851899658No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCACCTTGGTACCTC22270.2256163165071717No Hit
CCTTGGTACCTCCGCCGAGCACCACGAGAGTGTTGGTTGCATATGAGCTGCAATAATAATCAGCCTCGTCCTCAG21720.22004429252518048No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCCCTCACCATCTCCTG20680.2095081017228698No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19550.1980601251780515No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18860.19106976781882615No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18690.18934750586075613No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17830.18063488654346077No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCA17750.17982441032789842No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16720.16938952905253304No Hit
GTACCAAGGTGACCGTCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG16490.16705940993279125No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16340.16553976702861184No Hit
CCACGAGAGTGTTGGTTGCATATGAGCTGCAATAATAATCAGCCTCGTCC15460.15662452865742588No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15240.1543957190646294No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15200.15399048095684822No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15150.15348393332212174No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15140.15338262379517645No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14930.15125512372932526No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14710.14902631413652875No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14670.1486210760287476No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG14640.14831714744791172No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATGACTTTGTCTCCTGGTACCGACACCTCCCAGGCAAAGC14550.14740536170540405No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14140.14325167110064696No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12940.13109452786721157No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12800.12967619448997744No Hit
CCACCAAACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12790.12957488496303216No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12360.1252185753043845No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCACC11930.1208622656457368No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11890.1204570275379556No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCAGCTCATAT11630.11782297983737794No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11480.11630333693319853No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11420.11569547977152675No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC11380.11529024166374557No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11360.115087622609855No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11110.11255488443622261No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCCCTCAC11070.11214964632844143No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11050.11194702727455084No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10840.10981952720869965No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC10780.10921167004702788No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10760.10900905099313729No Hit
CTCTCGTGGTGCTCGGCGGAGGTACCAAGGTGACCGTCGTAGGTCAGCCC10640.10779333666979375No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10530.10667893187339551No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10480.10617238423866904No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10100.10232262221474783No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10070.10201869363391194No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10060.10191738410696666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2000.045.8431361
GGTATCA10750.045.4877621
CGTATGC901.200533E-1038.42194741
GTATCAA29050.037.406271
TCGTATG952.1827873E-1036.33226840
TCTCGTA1003.947207E-1034.38640238
TATCTCG1004.0927262E-1034.2686236
TATCAAC32150.033.5864942
AATGGTA6600.032.85923870
ATCAACG33050.032.7746283
ATCTCGT1056.8030204E-1032.6852437
GAGCCTA1155.4569682E-1132.60201331
TCAACGC33250.032.5774884
ATGCCGT1109.786163E-1031.5942344
CAACGCA34500.031.4955675
TGGTATC3150.031.2612252
AACGCAG35100.031.0539196
CCTATAC450.00499591230.183253
ACGCAGA37500.028.8868357
CGCAGAG37900.028.4923638
Produced by FastQC (version 0.11.5)