FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987074
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATGACTTTGTCTCCTGGTACCGACACCT36210.3668417970689128No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35690.36157370166775743No Hit
CTCCTGGACAGTCCCTCACCATCTCCTGCACTGGAACCAGCAGTGACATT34850.3530637014043526No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26510.26857155593197674No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCCCTCACCATCTCCTG26420.26765977018946907No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23130.23432893582446707No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21340.21619453050125925No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCAGCTCATAT19650.19907322044750445No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATGACTTTGTCTCCTGGTACCGACACCTCCCAGGCAAAGC19580.19836405375888735No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19160.194109053627185No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19060.19309595835773205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17920.1815466722859684No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17890.18124274370513255No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17610.1784060769506643No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16870.17090917195671246No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16790.1700986957411501No Hit
GTACCAAGGTGACCGTCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG16030.1623991716933077No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15780.15986643351967533No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCA15550.15753631439993354No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCCCTCAC15330.15530750480713706No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15170.15368655237601234No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14870.1506472665676535No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14160.14345429015453756No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG14030.14213726630424872No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13940.14122548056174106No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13530.13707178995698396No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12880.13048667070553982No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12780.12947357543608687No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12620.12785262300496214No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12330.12491464672354857No Hit
CTCTCGTGGTGCTCGGCGGAGGTACCAAGGTGACCGTCGTAGGTCAGCCC12030.12187536091518975No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCCCTCACCATCT11850.12005178943017443No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11690.11843083699904973No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC11360.115087622609855No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11320.1146823845020738No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC11200.11346667017873026No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11120.1126561939631679No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCCCTCAC10920.11063000342426202No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10910.11052869389731673No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10850.10992083673564494No Hit
GATTATTATTGCAGCTCATATGCAACCAACACTCTCGTGGTGCTCGGCGGAGGTACCAAGGTGACCGTCGTAGGT10270.10404488417281785No Hit
GGCCTGGCCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10260.10394357464587256No Hit
CCACCAAACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10190.10323440795725548No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10120.1025252412686384No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC10040.10171476505307607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)