FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467145
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57320.39069076335331543No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54380.3706518442280756No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA46460.3166694498498785No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45480.3099898101414652No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40950.27911351638726917No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGCT38630.26330049177143366No Hit
GTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT38570.26289153423826545No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37880.2581885226068316No Hit
CTCCCGGCACCGTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC35470.24176206169124387No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34910.23794512471500773No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33390.227584867208081No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAACTTCTTAGCCTGGTACC33000.22492664324248796No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32780.22342713228753805No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32270.21995099325560868No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31760.21647485422367935No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGACGATT31330.21354399190264087No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30750.20959073574868198No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30660.2089772994489297No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29540.2013434254964574No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA29380.20025287207467565No Hit
CTACCATACTGCTGACAGAAATATACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC28660.19534538167665771No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27010.18409904951453332No Hit
GGCCAAAAGTGTACGGTGAGCTACCATACTGCTGACAGAAATATACTGCA26900.18334929403705835No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26480.18048659130488123No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25990.17714677145067462No Hit
GAAATATACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25980.17707861186181326No Hit
TCTATGATGCGTCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT25980.17707861186181326No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25880.17639701597319965No Hit
GTATATTTCTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA25640.17476118584052702No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25610.1745567070739429No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA25400.17312535570785437No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24720.1684905036652819No Hit
CTCCCAGGCTCCTCATCTATGATGCGTCCAGCAGGGCCGCTGGCATCCCA24220.1650825242222139No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG23430.15969791670216646No Hit
GTATTACTGTGCTCGCGAGCCCGGGTCGTGGGTGGGAGTCACGGCAGGGTGGGGCCAGGGAAGCCTGGTCACCGT23210.15819840574721653No Hit
CATCATAGATGAGGAGCCTGGGAGTCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGAAGTTGCTGCCAACACT22260.15172324480538735No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT21920.14940581878410109No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC21640.14749735029598302No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21630.14742919070712165No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21520.1466794352296467No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21410.14592967975217175No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT21380.14572520098558767No Hit
GTCAGAGTGTTGGCAGCAACTTCTTAGCCTGGTACCAGCAGAAGCCTGGC20890.142385381131381No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20730.14129482770959928No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGACGATTTTGCAGTATATTTCTGTCAGCAGTATGGTAGCTCA20680.14095402976529245No Hit
TCATAGATGAGGAGCCTGGGAGTCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGAAGTTGCTGCCAACACTCTGA20570.1402042742878175No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20540.13999979552123343No Hit
CAGTAATACACGGCCGTGTCTTCAGATCTCAGGCTGGTCAGGTCCATGTA20320.1385002845662835No Hit
GTTCATCACTGTCCTCAATAAAGGGGACTATGCAGAGAAGTTCCAGGGCAGAGTCATCCTTACGGCGGACAAATC20310.13843212497742213No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20100.1370007736113336No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20090.13693261402247223No Hit
CATCTATGATGCGTCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA19770.13475150717890869No Hit
GTGCCGGGAGACTGTGTCAAAACAATTCCTCCGGTGGTATCTGGGAGCCA19680.13413807087915647No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19330.13175248526900885No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19120.1303211339029203No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGGAATTGTTTTGACACAGTCTC19110.13025297431405894No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18580.12664051610440685No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGGAATTGTTTTGACACAGTC18570.1265723565155455No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18270.1245275688497047No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18110.12343701542792294No Hit
GCCTGGGAGTCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGAAGTTGCTG17940.12227830241727981No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACACGGTGCCGGG17630.12016535516257765No Hit
GCAGAAGCCTGGCCAGACTCCCAGGCTCCTCATCTATGATGCGTCCAGCAGGGCCGCTGGCATCCCAGACAGGTT17420.1187340037964891No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17250.11757529078584598No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17140.11682553530837103No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17020.1160076202420347No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17010.11593946065317334No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17000.11587130106431198No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16470.1122588428546599No Hit
GTGTATTACTGTGCTCGCGAGCCCGGGTCGTGGGTGGGAGTCACGGCAGG16410.11184988532149175No Hit
ACCATACTGCTGACAGAAATATACTGCAAAATCGTCAGGCTCCAGTCTGC16320.11123644902173949No Hit
GTCTCCCGGCACCGTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG16230.11062301272198725No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16200.11041853395540317No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16120.1098732572445123No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16030.10925982094476007No Hit
GTCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG16010.10912350176703735No Hit
GTCTGGGATGCCAGCGGCCCTGCTGGACGCATCATAGATGAGGAGCCTGGGAGTCTGGCCAGGCTTCTGCTGGTA15810.10776030998981014No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15760.10741951204550333No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15670.1068060757457511No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15670.1068060757457511No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15630.10653343739030566No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15530.10585184150169206No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15530.10585184150169206No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15400.10496576684649439No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15380.10482944766877166No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15310.10435233054674214No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15290.10421601136901942No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15280.10414785178015806No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15250.10394337301357398No Hit
GGCTTCTGCTGGTACCAGGCTAAGAAGTTGCTGCCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC15150.10326177712496039No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15090.10285281959179222No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15070.1027165004140695No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15010.10230754288090135No Hit
ATGCAGAGAAGTTCCAGGGCAGAGTCATCCTTACGGCGGACAAATCCACG14910.10162594699228773No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14890.10148962781456504No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAAGGTTCATCACTGTCCTCAAT14880.10142146822570366No Hit
CTGCTGGACGCATCATAGATGAGGAGCCTGGGAGTCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGAAGTTGCTG14830.10108067028139686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3150.050.3325741
TCGTATG3250.049.75401740
GACTAGG3300.047.97640626
ACGAGAC3400.046.5161122
AGACTAG3500.045.2225525
CGAGACT3550.044.55671723
GGTATCA7650.043.9628751
GAGACTA3600.043.94986724
ATCTCGT3350.043.84135437
CCGTCTT3750.043.80595447
CTTATAC3550.043.5789761
ATGCCGT3800.042.00476544
GATCTCG3500.041.88270636
GCCGTCT3950.041.48653446
CACGAGA3900.040.54144321
TATACAC3900.040.5303883
GTATCAA20400.040.3908921
ACTAGGC3950.040.1007227
ATCTCCG3950.040.01734510
TACACAT4000.039.5171285
Produced by FastQC (version 0.11.5)