FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467145
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53770.3664941093075327No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50070.34127506142882946No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44510.3033783300219133No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43750.2981982012684499No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43740.2981300416795886No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA42870.29220015744865024No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37080.2527357554979228No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGCT37050.25253127673133874No Hit
GTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT36850.2511680849541115No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36120.24619243496723225No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34440.2347416240385238No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGACGATT33250.22663063296402197No Hit
CTCCCGGCACCGTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC33170.2260853562531311No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAACTTCTTAGCCTGGTACC33090.22554007954224017No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31690.2159977371016498No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30590.2085001823269002No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29920.20393348987318907No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA28960.1973901693424985No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28760.19602697756527132No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28170.19200556182245107No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27860.18989261456774892No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27290.1860075180026514No Hit
GGCCAAAAGTGTACGGTGAGCTACCATACTGCTGACAGAAATATACTGCA27110.18478064540314693No Hit
CTACCATACTGCTGACAGAAATATACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC26790.1825995385595834No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26550.18096370842691079No Hit
GTATATTTCTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA25990.17714677145067462No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA25930.17673781391750645No Hit
GAAATATACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25050.17073977009770677No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24720.1684905036652819No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG24570.1674681098323615No Hit
TCTATGATGCGTCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT24360.16603675846627294No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24340.1659004392885502No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23160.15785760780290972No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23130.15765312903632567No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT22160.1510416489167737No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21950.14961029755068517No Hit
CTCCCAGGCTCCTCATCTATGATGCGTCCAGCAGGGCCGCTGGCATCCCA21950.14961029755068517No Hit
GTATTACTGTGCTCGCGAGCCCGGGTCGTGGGTGGGAGTCACGGCAGGGTGGGGCCAGGGAAGCCTGGTCACCGT21800.14858790371776479No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21620.1473610311182603No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21530.14674759481850805No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC21310.14524808386355811No Hit
CATCATAGATGAGGAGCCTGGGAGTCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGAAGTTGCTGCCAACACT20930.14265801948682647No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20870.1422490619536583No Hit
GTTCATCACTGTCCTCAATAAAGGGGACTATGCAGAGAAGTTCCAGGGCAGAGTCATCCTTACGGCGGACAAATC20770.1415674660650447No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGACGATTTTGCAGTATATTTCTGTCAGCAGTATGGTAGCTCA20710.14115850853187653No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20510.13979531675464935No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19460.13263855992420653No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19340.1318206448578702No Hit
GTCAGAGTGTTGGCAGCAACTTCTTAGCCTGGTACCAGCAGAAGCCTGGC19150.13052561266950438No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19150.13052561266950438No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19000.12950321883658397No Hit
GTGCCGGGAGACTGTGTCAAAACAATTCCTCCGGTGGTATCTGGGAGCCA18960.12923058048113853No Hit
CAGTAATACACGGCCGTGTCTTCAGATCTCAGGCTGGTCAGGTCCATGTA18630.12698131404871366No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18540.1263678777489614No Hit
TCATAGATGAGGAGCCTGGGAGTCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGAAGTTGCTGCCAACACTCTGA18430.12561812227148647No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGGAATTGTTTTGACACAGTCTC18310.12480020720515014No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18180.12391413254995245No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17980.12255094077272527No Hit
CATCTATGATGCGTCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17900.12200566406183437No Hit
GCCTGGGAGTCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGAAGTTGCTG17820.1214603873509435No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17670.1204379935180231No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGGAATTGTTTTGACACAGTC17670.1204379935180231No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17610.12002903598485493No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACACGGTGCCGGG17400.11859768461876638No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17110.11662105654178694No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17030.11607577983089605No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16830.11471258805366887No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16650.11348571545416437No Hit
GTCTCCCGGCACCGTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG16610.11321307709871893No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16470.1122588428546599No Hit
GCAGAAGCCTGGCCAGACTCCCAGGCTCCTCATCTATGATGCGTCCAGCAGGGCCGCTGGCATCCCAGACAGGTT16450.11212252367693719No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16080.10960061888906686No Hit
GTCTGGGATGCCAGCGGCCCTGCTGGACGCATCATAGATGAGGAGCCTGGGAGTCTGGCCAGGCTTCTGCTGGTA16020.10919166135589871No Hit
GGCTTCTGCTGGTACCAGGCTAAGAAGTTGCTGCCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC15970.1088508634115919No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15880.10823742711183967No Hit
GTGTATTACTGTGCTCGCGAGCCCGGGTCGTGGGTGGGAGTCACGGCAGG15660.10673791615688974No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15580.10619263944599887No Hit
GTCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG15440.10523840520193983No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15180.10346625589154446No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15090.10285281959179222No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15080.10278466000293086No Hit
GTTTTGACACAGTCTCCCGGCACCGTGTCTTTGTCTCCAGGGGAAAGAGC15070.1027165004140695No Hit
ACCATACTGCTGACAGAAATATACTGCAAAATCGTCAGGCTCCAGTCTGC14940.10183042575887181No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14800.10087619151481278No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14770.1006717127482287No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14680.10005827644847647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)