FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664058
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATGTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCTTCAGC39140.5894063470359516No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34470.5190811645970683No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT33260.5008598646503769No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGTGACCGTGGTCCCA30880.4650196217800252No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC30310.4564360342018317No Hit
CACATACACCGTCTTCGGAAGTGGGACCACGGTCACCGTCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACT29710.4474006788563649No Hit
TCTCTGGACTCCAGGCTGAAGATGAGGCTGATTATTACTGCTGCTCATAT26850.4043321517096399No Hit
GATTATTACTGCTGCTCATATGCAGGCACATACACCGTCTTCGGAAGTGGGACCACGGTCACCGTCCTCGGTCAG26690.40192272361751535No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG26150.3937909038065952No Hit
GCCTTGGGCTGACCGAGGACGGTGACCGTGGTCCCACTTCCGAAGACGGT25790.3883696905993151No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25240.3800872815326372No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC23570.3549388758210879No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCGTTGGATCCAG22550.33957877173379436No Hit
ATCCAGTACTGATGTCGGTGGTTACAACTATGTCTCCTGGTACCAACACC21520.324068078390743No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTCCCCGGGAGCTGTGACAGTGGC21430.32271277508892293No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC20640.31081622388405833No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGCTGCCAGACTTGGAGCCAGAGAAG20510.30885856355920716No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGTCCAGAGATGGTCAGGGAGG19790.29801613714464703No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19360.29154079914706243No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCGTTGGATCCAGTACTGATGTC19170.2886796032876646No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18580.27979483719795556No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCGCTCAGGGCACAGGATCCTGGG18370.27663246282704224No Hit
GTACCAACACCTCCCAGGCAAAGCCCCCAAACTCATTATTTATGATGTCA18310.27572892729249554No Hit
GTGTTGGTACCAGGAGACATAGTTGTAACCACCGACATCAGTACTGGATCCAACGCAGGAGATGGTGACTGA17190.2588629306476241No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17070.2570558595785308No Hit
GTATGTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCTTCAGCCTGGAGTCCAGAGATGGTCAGGGAGGC15610.23506982823789488No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGTGACCGTGGTCCCACTTCCGAAGACGGTGTATGTGC15570.23446747121486375No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG15500.2334133464245593No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15430.23235922163425482No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15060.22678741917121695No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCGCTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC14950.22513093735788142No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14570.21940854563908574No Hit
GGCACATACACCGTCTTCGGAAGTGGGACCACGGTCACCGTCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACT14290.21519204647786788No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG14230.21428851094332121No Hit
GTCAGTCACCATCTCCTGCGTTGGATCCAGTACTGATGTCGGTGGTTACAACTATGTCTCCTGGTACCAACACCT13560.20419903080754997No Hit
GGTTACAACTATGTCTCCTGGTACCAACACCTCCCAGGCAAAGCCCCCAAACTCATTATTTATGATGTCACTAAG13180.1984766390887543No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12810.19290483662571642No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12730.1917001225796542No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATAATGAGTTTGGGGGCTTTGCCTGGGAGGTGTTGGTACCAGGAGA12690.19109776555662308No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCGTTGGATCCAGTA12260.18462242755903852No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12240.18432124904752295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12200.18371889202449182No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12050.18146005318812514No Hit
GCCCTCAGGGGTCCCTGGTCGCTTCTCTGGCTCCAAGTCTGGCAGCACGG11470.17272587635417389No Hit
CCTCAGGGGTCCCTGGTCGCTTCTCTGGCTCCAAGTCTGGCAGCACGGCCTCCCTGACCATCTCTGGACTCCAGG11160.1680576094256827No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11070.16670230612386266No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCGTTGGATCCAGTACTGATGT11040.16625053835658934No Hit
CTAGTGTGTCTGATCAGTGACTTCTCCCCGGGAGCTGTGACAGTGGCCTG11010.165798770589316No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10650.1603775573820359No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10630.16007637887052034No Hit
GTACTGGATCCAACGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGA10540.15872107556870033No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10330.15555870119778695No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCG10310.15525752268627138No Hit
TCCGAAGACGGTGTATGTGCCTGCATATGAGCAGCAGTAATAATCAGCCT10290.15495634417475582No Hit
GTACTGATGTCGGTGGTTACAACTATGTCTCCTGGTACCAACACCTCCCAGGCAAAGCCCCCAAACTCATTATTT10140.1526975053383891No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10040.1511916127808113No Hit
CATCAGTACTGGATCCAACGCAGGAGATGGTGACTGACTGTCCAGGAGAC10030.15104102352505355No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9990.15043866650202242No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC9930.14953513096747575No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCGCTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT9890.14893277394444462No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9820.14787864915414015No Hit
GTACTGATGTCGGTGGTTACAACTATGTCTCCTGGTACCAACACCTCCCA9820.14787864915414015No Hit
TCCTCAGCCTCCTCGCTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT9790.14742688138686683No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9690.145920988829289No Hit
CTGTCACAGCTCCCGGGGAGAAGTCACTGATCAGACACACTAGTGTGGCC9670.14561981031777346No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTCCCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC9590.1444150962717112No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGGAGAAGTCACTGAT9510.14321038222564897No Hit
CAACTATGTCTCCTGGTACCAACACCTCCCAGGCAAAGCCCCCAAACTCATTATTTATGATGTCACTAAGCGGCC9310.14019859711049334No Hit
GTCTTCGGAAGTGGGACCACGGTCACCGTCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCC9280.13974682934322002No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC9250.13929506157594668No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9250.13929506157594668No Hit
GTCACAGCTCCCGGGGAGAAGTCACTGATCAGACACACTAGTGTGGCCTT8980.13522915167048663No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8840.1331209020898777No Hit
GCTCATATGCAGGCACATACACCGTCTTCGGAAGTGGGACCACGGTCACCGTCCTCGGTCAGCCCAAGGCCAACC8810.13266913432260435No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8710.13116324176502656No Hit
GACCGAGGACGGTGACCGTGGTCCCACTTCCGAAGACGGTGTATGTGCCTGCATATGAGCAGCAGTAATAATCAG8700.13101265250926877No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGGAGAAGTCACTGATCAGACACACTAGTGTGGC8580.1292055814401754No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8560.12890440292865984No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGGAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8530.1284526351613865No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8390.12634438558077757No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8170.1230314219541064No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA8120.12227847567531751No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG8090.12182670790804417No Hit
CCTGAGGGCCGCTTAGTGACATCATAAATAATGAGTTTGGGGGCTTTGCC8050.12122435088501307No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8000.12047140460622416No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCGCTCAGGGCACAGGATCCTGGGCTCAGTCTGC7960.11986904758319304No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA7930.11941727981591971No Hit
CTCCTGCGTTGGATCCAGTACTGATGTCGGTGGTTACAACTATGTCTCCTGGTACCAACACCTCCCAGGCAAAGC7860.11836315502561523No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7710.11610431618924853No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC7670.1155019591662174No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG7660.11535136991045963No Hit
GTCCAGGAGACCCGGACACTGAGCGAGGCTGAGTCAGGGCAGACTGAGCC7610.11459842363167073No Hit
GTTTGGGGGCTTTGCCTGGGAGGTGTTGGTACCAGGAGACATAGTTGTAACCACCGACATCAGTACTGGATCCAA7460.11233958479530401No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG7450.11218899553954624No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG7420.1117372277722729No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGG7410.11158663851651511No Hit
GGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGT7390.11128546000499957No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG7390.11128546000499957No Hit
GTTGTAACCACCGACATCAGTACTGGATCCAACGCAGGAGATGGTGACTG7360.11083369223772621No Hit
CTGCTCCTCCTCAGCCTCCTCGCTCAGGGCACAGGATCCTGGGCTCAGTC7300.10993015670317954No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG7210.10857485340135951No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC7070.10646660382075059No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGGAGAAGTCACTGA7060.10631601456499282No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT6910.10405717572862612No Hit
AGGCAGCACTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCT6870.10345481870559499No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT6790.10225010465953276No Hit
GTCTGGCAGCACGGCCTCCCTGACCATCTCTGGACTCCAGGCTGAAGATG6790.10225010465953276No Hit
CATACACCGTCTTCGGAAGTGGGACCACGGTCACCGTCCTCGGTCAGCCC6750.10164774763650163No Hit
CTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAG6660.10029244433468161No Hit
GCAGTAATAATCAGCCTCATCTTCAGCCTGGAGTCCAGAGATGGTCAGGG6650.10014185507892383No Hit
GACCATCTCTGGACTCCAGGCTGAAGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCACATACACCGTCTT6650.10014185507892383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)