FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297362
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATGCTTTCCCTCCGTACACTTTTGGCCAGGGGACCAAGTTAGAAATCAAACGAACTGTGGCTGCACCAT62890.4847529062821325No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62400.48097601132143536No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60840.4689516110383995No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48320.3724480908181371No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44410.3423100106215536No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG42170.32504420508693793No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39020.30076416605388473No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37040.2855024272331084No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37040.2855024272331084No Hit
GCATCATACTGTTGACAGTAATATGTCGCAATATCTTCAGGCTCCAGGCTGCTGATGGTGAAAGTAAAATCTGTC36780.28349836051926913No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36700.28288172460731853No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34580.2665408729406288No Hit
AGTCAGGACATTGGCAAGTATGTAAATTGGTATCAGCAGAAACCAGGGAA33510.25829336761829No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32460.250200021273939No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG32390.24966046485098223No Hit
CTTTTGGCCAGGGGACCAAGTTAGAAATCAAACGAACTGTGGCTGCACCA31870.2456523314233036No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31820.2452669339783345No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31520.2429545493085199No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31300.24125880055065585No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30530.23532367989813177No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29430.22684493610881157No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29090.2242242334830217No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27830.2145122178698004No Hit
GTATGTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGA26930.20757506386035662No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26090.20110038678487577No Hit
GGCCAAAAGTGTACGGAGGGAAAGCATCATACTGTTGACAGTAATATGTCGCAATATCTTCAGGCTCCAGGCTG25870.19940463802701172No Hit
GTGCAGCCACAGTTCGTTTGATTTCTAACTTGGTCCCCTGGCCAAAAGTG25250.1946257097093949No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTACATACTTGCCA24210.18660944285403766No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCTAACTTGGTCC23930.1844512171622107No Hit
ATATTACTGTCAACAGTATGATGCTTTCCCTCCGTACACTTTTGGCCAGGGGACCAAGTTAGAAATCAAACGAA23790.18337210431629722No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC23550.18152219658044555No Hit
CAGTAATATGTCGCAATATCTTCAGGCTCCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA23480.18098264015748883No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT23070.1778223811087422No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22960.1769745067298102No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22730.17520167848295234No Hit
GATCTACGATGCATCCGATTTGGATACGGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA22230.17134770403326136No Hit
GTAATATGTCGCAATATCTTCAGGCTCCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA21560.16618337827067542No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21440.16525842440274957No Hit
GTCCTGATCTACGATGCATCCGATTTGGATACGGGGGTCCCATCAAGGTT21390.1648730269577805No Hit
GTAGGAGACACAGTCACCATCACTTGCCAGGCGAGTCAGGACATTGGCAAGTATGTAAATTGGTATCAGCAGAAA21120.16279188075494733No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACTGTGTCTCCTACAGATGCAGACAGGGAGGAAGGCGACTGGGTCAT20930.16132737046406476No Hit
TCGTAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT20800.1603253371071451No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAGAGGACAGTTCCAGGGAGA20720.15970870119519456No Hit
ACATATTACTGTCAACAGTATGATGCTTTCCCTCCGTACACTTTTGGCCA20680.1594003832392193No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20670.15932330375022546No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT20660.15924622426123164No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTACAT20260.15616304470147885No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19740.1521549112738002No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCAGGCGAGTCAGG19380.14938004967002272No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19270.1485321752910907No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCGCCTTCCTCC18800.14490943930838115No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18590.14329077003951096No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18570.1431366110615233No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18340.14136378281466544No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18140.13982219303478904No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT17880.13781812632094975No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17750.1368160929640301No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17720.13658485449704863No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17690.13635361603006715No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17480.13473494676119696No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17470.13465786727220314No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17180.13242256209138237No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16890.1301872569105616No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16890.1301872569105616No Hit
GGATACGGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATT16820.12964770048760485No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16510.12725823632879643No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16460.12687283888382733No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16410.12648744143885823No Hit
ACCTTGATGGGACCCCCGTATCCAAATCGGATGCATCGTAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTG16290.12556248757093239No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16230.1251000106369695No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16180.12471461319200038No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15960.12301886443413636No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCGCCTTC15890.12247930801117962No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15740.12132311567627233No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15650.12062940027532792No Hit
CCCCTAAGGTCCTGATCTACGATGCATCCGATTTGGATACGGGGGTCCCA15650.12062940027532792No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGGAGCCTGAAGATATTG15070.11615878991368639No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14930.1150796770677729No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14880.11469427962280382No Hit
GATTTTACTTTCACCATCAGCAGCCTGGAGCCTGAAGATATTGCGACATATTACTGTCAACAGTATGATGCTTTC14830.11430888217783472No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14640.11284437188695215No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT14410.11107154364009429No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT14360.11068614619512518No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14300.11022366926116227No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14210.10952995386021788No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14110.1087591589702797No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13900.10714048970140946No Hit
CACCATCACTTGCCAGGCGAGTCAGGACATTGGCAAGTATGTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC13870.106909251234428No Hit
GGGTATATCTATTCCAGTGGAAACACCAAGTACAATCCCGCCCTCAATAG13810.1064467743004651No Hit
ACTGTCAACAGTATGATGCTTTCCCTCCGTACACTTTTGGCCAGGGGACCAAGTTAGAAATCAAACGAACTGTGG13760.106061376855496No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCG13600.10482810503159487No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13540.10436562809763197No Hit
GTTTATTACTGTGCGAGAGATCGAGGTCTTGTGCCCTCCTGGGGCCAGGG13480.10390315116366904No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13470.10382607167467522No Hit
TTTACATACTTGCCAATGTCCTGACTCGCCTGGCAAGTGATGGTGACTGT13370.10305527678473703No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13050.1005887331369348No Hit
CCTTCCTCCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCA13000.10020333569196571No Hit
ATTTACATACTTGCCAATGTCCTGACTCGCCTGGCAAGTGATGGTGACTG12980.10004917671397806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)