FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297362
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60750.46825789563745507No Hit
GTATGATGCTTTCCCTCCGTACACTTTTGGCCAGGGGACCAAGTTAGAAATCAAACGAACTGTGGCTGCACCAT60620.46725586228053545No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50900.3923345989785426No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46850.36111740593604563No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG46130.3555676827284906No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45290.3490930056530097No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38120.29382701204444095No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36180.27887359117963995No Hit
GCATCATACTGTTGACAGTAATATGTCGCAATATCTTCAGGCTCCAGGCTGCTGATGGTGAAAGTAAAATCTGTC35670.2749425372409551No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34770.26800538323151135No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33220.25605806243746926No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33110.25521018805853724No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32730.2522811674767721No Hit
CTTTTGGCCAGGGGACCAAGTTAGAAATCAAACGAACTGTGGCTGCACCA32040.24696268273619854No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31510.2428774698195261No Hit
AGTCAGGACATTGGCAAGTATGTAAATTGGTATCAGCAGAAACCAGGGAA30930.23840685945788453No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30930.23840685945788453No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30820.2375589850789525No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG29510.22746157202076212No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29460.22707617457579302No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29070.22407007450503405No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29020.22368467706006495No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28890.2226826437031453No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27300.21042700495312797No Hit
GTGCAGCCACAGTTCGTTTGATTTCTAACTTGGTCCCCTGGCCAAAAGTG26730.20603347408048026No Hit
GTATGTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGA26600.20503144072356055No Hit
GGCCAAAAGTGTACGGAGGGAAAGCATCATACTGTTGACAGTAATATGTCGCAATATCTTCAGGCTCCAGGCTG25710.1981713662031106No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24000.18499077358516744No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23950.18460537614019834No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23860.18391166073925397No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23510.18121387862447025No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCTAACTTGGTCC23150.17843901702069276No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTACATACTTGCCA22880.17635787081785964No Hit
ATATTACTGTCAACAGTATGATGCTTTCCCTCCGTACACTTTTGGCCAGGGGACCAAGTTAGAAATCAAACGAA22870.17628079132886582No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC22140.17065398863231696No Hit
CAGTAATATGTCGCAATATCTTCAGGCTCCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA21990.16949779629740966No Hit
GTCCTGATCTACGATGCATCCGATTTGGATACGGGGGTCCCATCAAGGTT21950.16918947834143439No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACTGTGTCTCCTACAGATGCAGACAGGGAGGAAGGCGACTGGGTCAT21590.16641461673765687No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT21510.16579798082570632No Hit
GTAATATGTCGCAATATCTTCAGGCTCCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA21360.16464178849079902No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21010.16194400637601533No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCAGGCGAGTCAGG20960.16155860893104623No Hit
GATCTACGATGCATCCGATTTGGATACGGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA20950.1614815294420524No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20470.15778171397034907No Hit
GTAGGAGACACAGTCACCATCACTTGCCAGGCGAGTCAGGACATTGGCAAGTATGTAAATTGGTATCAGCAGAAA20300.15647136265745412No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20180.1555464087895283No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTACAT19460.14999668558197327No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19100.14722182397819575No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19070.14699058551121427No Hit
TCGTAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT19030.146682267555239No Hit
ACATATTACTGTCAACAGTATGATGCTTTCCCTCCGTACACTTTTGGCCA18940.14598855215429463No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18870.1454489957313379No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAGAGGACAGTTCCAGGGAGA18830.14514067777536263No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT18810.14498651879737498No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCGCCTTCCTCC18770.1446782008413997No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18660.14383032646246768No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT18580.14321369055051714No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17960.1384347622329003No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17170.13234548260238854No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17120.13196008515741944No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17060.13149760822345652No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16970.13080389282251215No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCGCCTTC16560.12764363377376553No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16450.1267957593948335No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16440.12671867990583968No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16390.1263332824608706No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16330.1258708055269077No Hit
ACCTTGATGGGACCCCCGTATCCAAATCGGATGCATCGTAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTG16280.1254854080819386No Hit
CCCCTAAGGTCCTGATCTACGATGCATCCGATTTGGATACGGGGGTCCCA16190.12479169268099422No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16160.12456045421401275No Hit
GGATACGGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATT15760.12147727465425995No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15110.11646710786966166No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15020.11577339246871729No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT14720.1134610077989027No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14520.11191941801902631No Hit
GGGTATATCTATTCCAGTGGAAACACCAAGTACAATCCCGCCCTCAATAG14470.1115340205740572No Hit
GATTTTACTTTCACCATCAGCAGCCTGGAGCCTGAAGATATTGCGACATATTACTGTCAACAGTATGATGCTTTC14250.10983827181619316No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCG14210.10952995386021788No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14160.10914455641524878No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14100.10868207948128586No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14070.10845084101430441No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14000.10791128459134768No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13930.10737172816839094No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13820.10652385378945892No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13760.106061376855496No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13600.10482810503159487No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13570.10459686656461344No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13290.10243864087278647No Hit
GTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGGAGCCTGAAGATATTGCGACATATTA13220.10189908444982973No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13180.10159076649385444No Hit
ACTGTCAACAGTATGATGCTTTCCCTCCGTACACTTTTGGCCAGGGGACCAAGTTAGAAATCAAACGAACTGTGG13160.10143660751586682No Hit
GTTTATTACTGTGCGAGAGATCGAGGTCTTGTGCCCTCCTGGGGCCAGGG13080.10081997160391626No Hit
CCTTCCTCCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCA13070.10074289211492243No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGGC12980.10004917671397806No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG12980.10004917671397806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAAT400.00815594450.418970
GTGGTAT2950.037.7933461
TAGTCCG400.001915563536.7059264
TAGTGAT8900.034.7456170
GTATCAA20200.032.9486961
GGGCGTA851.4564648E-731.7878065
TCGGCTT450.00510607830.04961413
TATCAAC22450.028.8829042
TGTTAGA18450.027.69168370
ATCAACG23400.027.5659853
TCAACGC23750.027.3019474
GACAGGT1504.5474735E-1127.0279661
GCCTGAC6250.027.0269247
TACAGTA500.00858351127.0175512
CAACGCA24500.026.7439235
AACGCAG24650.026.5842576
GCTTGTA659.783576E-425.988431
GCACCGA4100.025.5437417
CGCAGAG25700.025.5079618
ACGCAGA25750.025.4525437
Produced by FastQC (version 0.11.5)