FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966700
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGCCCTGACCCTTTCGGGTGCGCAGC40160.41543395055342924No Hit
CTCCTATAGTGGTACATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCA39460.40819282093720904No Hit
CTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCCTTGCCC38910.4025033619530361No Hit
GTCCAGGACTGTTTGTTGTTTGTATCATAAATCAGTGTCCTGGGGGCTTG34500.3568842453708493No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT34410.35595324299162096No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA33470.34622944036412534No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG28740.2973000931002379No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC28000.2896451846488052No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26980.2790938243508844No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA24780.25633598841419264No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC24730.2558187648701769No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24370.2520947553532637No Hit
GCCTGAGGATGAGGCTGACTATTACTGCTTGCTCTCCTATAGTGGTACATATGTCTTCGGAACTGGGACCAAGGT23380.24185372918175235No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT23200.23999172442329572No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22680.2346125995655322No Hit
GACTATTACTGCTTGCTCTCCTATAGTGGTACATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCCGTCAG21800.2255094651908555No Hit
GTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG19470.20140684803972275No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG18760.19406227371469947No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18620.19261404779145547No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18310.189407261818558No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17710.18320057929036931No Hit
CTATTACTGCTTGCTCTCCTATAGTGGTACATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCCGTCAGCC17440.18040757215268438No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATGTACCA16880.1746146684597083No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16750.1732698872452674No Hit
GGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAG16710.17285610841005483No Hit
CCTATAGTGGTACATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCA16670.17244232957484223No Hit
AGGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGACTATTACTGCTTGCTCTCCTATAGTGGTA16630.17202855073962967No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16580.17151132719561393No Hit
CACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGC16390.1695458777283542No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15280.15806351505120514No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15280.15806351505120514No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGCCCTGACCCTTTCGGGTG15250.15775318092479568No Hit
GTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGCACTGGA15060.15578773145753594No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG14900.15413261611668563No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCAC14700.15206372194062273No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14480.14978793834695356No Hit
CAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGC13860.14337436640115858No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13780.14254680873073342No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13510.1397538015930485No Hit
GTGGTGACTCAGGAGCCCTCACTGAGTGTGTCCCCAGGAGGGACAGTCAC13390.13851246508741077No Hit
CATATGTACCACTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGG13110.1356160132409227No Hit
GTACATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCCGTCAGCCC13020.13468501086169443No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12960.13406434260887556No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12440.12868521775111205No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12000.12413365056377365No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11930.12340953760215166No Hit
CCCGAAAGGGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCG11890.12299575876693906No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC11810.12216820109651391No Hit
GCCTTGGGCTGACGGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA11790.12196131167890763No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11200.11585807385952207No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11190.11575462915071895No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11150.11534085031550637No Hit
CCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGACTATTACTGCTTGCTC10710.11078928312816799No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10650.11016861487534912No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG10590.10954794662253026No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10590.10954794662253026No Hit
GCACTGGAACTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAG10360.10716871832005792No Hit
GGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGG10350.1070652736112548No Hit
GCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGACTATTACTG10320.10675493948484535No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10280.10634116064963278No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC10190.10541015827040448No Hit
CTCACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA10150.1049963794351919No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10130.10478949001758558No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10100.10447915589117616No Hit
GAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGCACTGGAACTGTCACCAGTGGTCATTATCC9830.10168614875349126No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9770.10106548050067239No Hit
CAACAAACAGTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGG9740.10075514637426296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG200.006964734352.4750450
CGTATGC1800.047.9918941
TCGTATG1750.047.33892440
CGGGTAT405.367471E-542.6118246
CTTATAC2600.041.9562571
TCTCGTA1850.040.9604338
TATACAC2700.040.402323
CCGTCTT2200.039.57316647
TTAACGG350.001380994639.23334535
ATCTCGT1950.038.81729537
ACGAGAC2400.038.35857822
AGGGGGG3150.038.0059470
GCCGTCT2350.037.01201646
TCCGAGC2500.036.8147113
TTATACA2900.036.4404562
ATGCCGT2400.036.1474344
TCCGAGA4600.036.14695470
CACGAGA2550.036.0965921
TGCCGTC2450.035.44705645
CGACCAA400.002334666235.2547155
Produced by FastQC (version 0.11.5)