FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966700
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGCCCTGACCCTTTCGGGTGCGCAGC41460.428881762697838No Hit
CTCCTATAGTGGTACATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCA40380.4177097341470984No Hit
CTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCCTTGCCC36820.38088341781317886No Hit
GTCCAGGACTGTTTGTTGTTTGTATCATAAATCAGTGTCCTGGGGGCTTG33180.3432295438088342No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA32140.3324712940933071No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT31530.3261611668563153No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC30790.3185062584048826No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA26600.27516292541636495No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC26510.27423192303713667No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG26410.2731974759491052No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26220.2712320264818454No Hit
GCCTGAGGATGAGGCTGACTATTACTGCTTGCTCTCCTATAGTGGTACATATGTCTTCGGAACTGGGACCAAGGT23780.24599151753387813No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT22780.23564704665356367No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC21450.22188890038274545No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20440.21144098479362777No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20390.2109237612496121No Hit
GACTATTACTGCTTGCTCTCCTATAGTGGTACATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCCGTCAG20390.2109237612496121No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19640.20316540808937622No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19160.19820006206682528No Hit
GTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG18580.1922002689562429No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17290.17885590152063724No Hit
CTATTACTGCTTGCTCTCCTATAGTGGTACATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCCGTCAGCC16640.17213199544843283No Hit
GGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAG16560.17130443777800763No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT16380.16944243301955103No Hit
GTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGCACTGGA16340.16902865418433846No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATGTACCA15880.16427019757939382No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15720.16261508223854348No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15720.16261508223854348No Hit
CACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGC15680.16220130340333092No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15550.16085652218889004No Hit
AGGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGACTATTACTGCTTGCTCTCCTATAGTGGTA15490.16023585393607115No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15460.15992551980966174No Hit
CCTATAGTGGTACATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCA15340.158684183304024No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCAC15270.15796007034240198No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGCCCTGACCCTTTCGGGTG15060.15578773145753594No Hit
GTGGTGACTCAGGAGCCCTCACTGAGTGTGTCCCCAGGAGGGACAGTCAC13750.14223647460432398No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13440.1390296886314265No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13310.1376849074169856No Hit
CAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGC13150.1360297920761353No Hit
CATATGTACCACTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGG13030.13478845557049757No Hit
GTACATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCCGTCAGCCC12970.1341677873176787No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12860.13302989552084413No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC12690.13127133547119063No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12600.13034033309196233No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12570.13002999896555292No Hit
GCCTTGGGCTGACGGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA12410.1283748836247026No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12160.12578876590462398No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12160.12578876590462398No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11930.12340953760215166No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11670.12071997517326988No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11310.11699596565635667No Hit
CCCGAAAGGGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCG10980.1135822902658529No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10740.11109961725457743No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10520.10882383366090824No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT10420.10778938657287679No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG10370.10727216302886108No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10360.10716871832005792No Hit
GAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGCACTGGAACTGTCACCAGTGGTCATTATCC10080.10427226647356988No Hit
GGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGG9940.10282404055032585No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC9930.1027205958415227No Hit
GCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGACTATTACTG9770.10106548050067239No Hit
CCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGACTATTACTGCTTGCTC9740.10075514637426296No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC9700.10034136753905037No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9680.10013447812144409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT701.0725344E-634.07475716
TTCTCGG800.002978436433.5433770
AATGGTA5600.032.34539470
TATCAAC30300.031.01112
GTATCAA31450.030.966061
CAGTAGG14100.030.92651270
CCGTATT450.004908914730.29181114
TCAACGC31000.030.0896474
ATCAACG31350.029.8623093
CAACGCA31700.029.53265
AACGCAG32000.029.26336
CGCAGAG33300.028.028788
ACGCAGA33450.028.000587
ATAGGTG4400.027.8533924
AGAGTAC33700.027.60644511
CGGGATT500.00822092827.25698317
CAGAGTA34350.027.0826510
GCAGAGT34550.027.018919
GTCATAG4700.026.8067671
GTTCAAT659.342726E-426.1937221
Produced by FastQC (version 0.11.5)