FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206205
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACAGCTTCCTATCACCTTCGGCCCAGGGACACGACTGGAGATTGAA102940.8534204384826792No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81500.6756728748429993No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64150.5318333119162995No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47480.3936312650005596No Hit
GTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAAGTTT44830.3716615334872596No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44570.36950601265953964No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATT43920.36411721059023966No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43660.36196168976251963No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42140.3493601833850796No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG41090.3406551954269797No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTG40540.33609543982987966No Hit
GCTGTATACTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT39340.32614688216347965No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38990.32324521951077967No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36970.3064984807723397No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33270.2758237613009397No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32240.2672845826372798No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30570.25343950655153974No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30400.2520301275487997No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30200.2503720346043997No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29430.24398837676845977No Hit
CTCCTGCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGAT27730.22989458674105975No Hit
CCAGAGAACCGCTTGGAAACTTCATAGATCAGGAGCTGTGGAGGCTGGCC27350.22674421014669977No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27340.22666130549947977No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26770.2219357406079398No Hit
GTGCAGCCACAGTTCGTTCAATCTCCAGTCGTGTCCCTGGGCCGAAGGTG26510.21978021978021978No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26240.2175417943052798No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGCTCCAGTGCACATATTGTGATGA25100.20809066452219976No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA25070.20784195058053978No Hit
TTCCATCACTATGCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCC24350.20187281598069978No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23900.1981421068557998No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23380.1938310652003598No Hit
TTATTACTGCATGCAAAGTATACAGCTTCCTATCACCTTCGGCCCAGGGA23060.19117811648931982No Hit
AGCCTCCACAGCTCCTGATCTATGAAGTTTCCAAGCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGT23010.19076359325321982No Hit
GGATACCTGGCTCCAGTGCACATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGG22820.1891884049560398No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTA22770.1887738817199398No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT22680.1880277398949598No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA22050.1828047471200998No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21980.18222441458955982No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21800.1807321309395998No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGG21770.18048341699793982No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21510.17832789617021982No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCAATCTCCAGTCGTGTCCCTGGGCCGAAGGTGATAGGAAG21310.17666980322581982No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21210.17584075675361982No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21090.17484590098697983No Hit
TCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGG20990.17401685451477983No Hit
GTGCACATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCT20550.17036905003709982No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA20500.16995452680099984No Hit
GTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATG20160.16713576879551983No Hit
GGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGAT20140.16696995950107985No Hit
GTTTATTACTGCATGCAAAGTATACAGCTTCCTATCACCTTCGGCCCAGGGACACGACTGGAGATTGAACGAA19910.16506315261501983No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGCTCCAGTGCACATATTGTGATGACCCAGACTCCACTCTCT19380.16066920631235984No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19170.15892820872073984No Hit
GCTAATGCTCTGGATACCTGGCTCCAGTGCACATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCC18860.15635816465691985No Hit
ATGCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCCAGGGGTGACGGACAGAGAGAGTGGAGT18840.15619235536247983No Hit
CGACTGGAGATTGAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT18600.15420264382919985No Hit
AGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTC18560.15387102524031984No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18490.15329069270977985No Hit
CCTGCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAG18450.15295907412089985No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18440.15287616947367985No Hit
ACTGGAGATTGAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC18170.15063774399873983No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGCTCCAGTGCACATATTGTGATGACCCAGACTCCACT17870.14815060458213986No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17860.14806769993491983No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17730.14698993952105985No Hit
GTGGAGGCTGGCCTGGCTTCTGCAGGTACCAATACAAATAGGTCTTTCCA17670.14649251163773985No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17590.14582927445997987No Hit
GTCCAGGGGTGACGGACAGAGAGAGTGGAGTCTGGGTCATCACAATATGT17540.14541475122387984No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGCTCCAGTGCACATATTGTGATGACCCAGACTC17470.14483441869333985No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTGT17440.14458570475167984No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17390.14417118151557987No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17250.14301051645449986No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC17200.14259599321839986No Hit
GCCCAGGGACACGACTGGAGATTGAACGAACTGTGGCTGCACCATCTGTC16800.13927980732959985No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16640.13795333297407986No Hit
GTGTATTACTGTGCGAGAGGCGGGGAGCATGATAGTAGTGGTTATTACGGACGCATGACAACACTTGACTACTA15860.13148677049091986No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15830.13123805654925985No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15800.13098934260759987No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15640.12966286825207987No Hit
AGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAAGTTTCCAAGCGGTTC15600.12933124966319987No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15480.12833639389655987No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15390.12759025207157987No Hit
GTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAAAATCAGCCGGG15270.12659539630493988No Hit
CTCCAGAGAACCGCTTGGAAACTTCATAGATCAGGAGCTGTGGAGGCTGG15270.12659539630493988No Hit
CCACTCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAA15220.12618087306883988No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15170.12576634983273988No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15150.1256005405382999No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGCTCCAGTGCA14980.12419116153555988No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA14630.12128949888285988No Hit
CCTTCGGCCCAGGGACACGACTGGAGATTGAACGAACTGTGGCTGCACCA14530.12046045241065988No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14410.11946559664401989No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14330.11880235946625987No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14320.11871945481903988No Hit
ATCTGGCACTCCAGAGAACCGCTTGGAAACTTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGCAGGTA14260.11822202693571987No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14250.11813912228849988No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14180.11755878975795989No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14060.1165639339913199No Hit
GCCACAGTTCGTTCAATCTCCAGTCGTGTCCCTGGGCCGAAGGTGATAGG14020.11623231540243988No Hit
CCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTC13740.11391098528027988No Hit
ATCTCCAGTCGTGTCCCTGGGCCGAAGGTGATAGGAAGCTGTATACTTTGCATGCAGTAATAAACCCCAACATC13620.11291612951363988No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13510.11200417839421989No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13510.11200417839421989No Hit
GTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAA13470.1116725598053399No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13440.1114238458636799No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13330.1105118947442599No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13330.1105118947442599No Hit
GCAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAAGTTTCCAAGCGGTTCTCTGGAGTGCCAGATAGGTT13330.1105118947442599No Hit
GCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCC12990.1076931367387799No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12970.10752732744433989No Hit
GCTCCTGATCTATGAAGTTTCCAAGCGGTTCTCTGGAGTGCCAGATAGGT12950.1073615181498999No Hit
GGACACGACTGGAGATTGAACGAACTGTGGCTGCACCATCTGTCTTCATC12870.10669828097213989No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12660.10495728338051988No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12500.1036308090249999No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12450.10321628578889988No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12300.10197271608059989No Hit
GATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGCAGGTACCAATACAAAT12280.1018069067861599No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG12230.10139238355005989No Hit
GATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAAGTATACAGCTT12180.1009778603139599No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12160.1008120510195199No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12090.1002317184889799No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGT150.002448442268.330396
CGCAATT300.003194775263.89087370
CGCGTAT200.00737138251.72818839
ATCGCGT200.00742887451.62664437
GTCGTAA200.007573441651.3755930
GGTATCA8450.044.4754071
ACGCAAT408.944516E-442.85529769
GTATCAA24550.041.4713971
CTACGCT1000.041.06804328
ACTACGC1050.039.09781327
TACGCTG1100.037.3485429
GTGGTAT2600.036.7932851
TGGTATC2600.035.477772
TATCAAC29500.034.6270182
ATCAACG30200.034.0506553
TCAACGC30750.033.552724
CAACGCA31350.033.0195355
AACGCAG31450.032.9159136
ATAATAC851.3170029E-732.1541443
ATTCACT2250.031.8861942
Produced by FastQC (version 0.11.5)