FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206205
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACAGCTTCCTATCACCTTCGGCCCAGGGACACGACTGGAGATTGAA95730.7936461878370592No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT79370.6580141849851393No Hit
GCTGTATACTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT53700.4451979555713996No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52720.43707330014383955No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48250.4000149228364996No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47400.3929680278227996No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45410.37647000302601963No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATT38350.3179393220886997No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38220.3168615616748397No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37090.3074933365389797No Hit
GTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAAGTTT36160.2997832043475197No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA35200.2918243582143997No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34790.2884252676783797No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33160.2749118101815197No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32380.26844524769835976No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31920.26463163392623973No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31330.2597402597402597No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29160.24174995129351973No Hit
GTGCAGCCACAGTTCGTTCAATCTCCAGTCGTGTCCCTGGGCCGAAGGTG28940.23992604905467974No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27470.22773906591333978No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27070.22442288002453978No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26770.2219357406079398No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26140.21671274783307978No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26080.21621531994975976No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25530.21165556435265978No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25240.20925132958327977No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTG25080.2079248552277598No Hit
CCAGAGAACCGCTTGGAAACTTCATAGATCAGGAGCTGTGGAGGCTGGCC25030.2075103319916598No Hit
CTCCTGCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGAT24150.20021472303629978No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24020.1991369626224398No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA22980.1905148793115598No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGG22430.1859551237144598No Hit
TTCCATCACTATGCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCC22280.18471155400615982No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21640.17940565658407984No Hit
GGATACCTGGCTCCAGTGCACATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGG21420.17758175434523982No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA21360.17708432646191982No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGCTCCAGTGCACATATTGTGATGA21010.17418266380921985No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCAATCTCCAGTCGTGTCCCTGGGCCGAAGGTGATAGGAAG20920.17343652198423984No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20800.17244166621759985No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20590.17070066862597985No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20560.17045195468431984No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTGT20550.17036905003709982No Hit
TTATTACTGCATGCAAAGTATACAGCTTCCTATCACCTTCGGCCCAGGGA20210.16755029203161984No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT20060.16630672232331983No Hit
GGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGAT19260.15967435054571982No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19010.15760173436521985No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18800.15586073677359985No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGCTCCAGTGCACATATTGTGATGACCCAGACTCCACTCTCT18530.15362231129865986No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18480.15320778806255986No Hit
GTTTATTACTGCATGCAAAGTATACAGCTTCCTATCACCTTCGGCCCAGGGACACGACTGGAGATTGAACGAA18340.15204712300147985No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18180.15072064864595985No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTA18030.14947707893765985No Hit
AGCCTCCACAGCTCCTGATCTATGAAGTTTCCAAGCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGT17930.14864803246545985No Hit
GTGCACATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCT17790.14748736740437984No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17760.14723865346271983No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17750.14715574881549984No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA17490.14500022798777987No Hit
GTCCCTGACCCGCTGCCACTGAACCTATCTGGCACTCCAGAGAACCGCTT17480.14491732334055984No Hit
GTGGAGGCTGGCCTGGCTTCTGCAGGTACCAATACAAATAGGTCTTTCCA17320.14359084898503985No Hit
CGACTGGAGATTGAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT17260.14309342110171985No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC17240.14292761180727986No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17110.14184985139341985No Hit
TCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGG17100.14176694674619986No Hit
GTCCAGGGGTGACGGACAGAGAGAGTGGAGTCTGGGTCATCACAATATGT16880.13994304450735987No Hit
GCCCAGGGACACGACTGGAGATTGAACGAACTGTGGCTGCACCATCTGTC16480.13662685861855986No Hit
GTATACTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCC16220.13447133779083986No Hit
GTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATG15980.13248162625755988No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15950.13223291231589987No Hit
ACTGGAGATTGAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC15920.13198419837423986No Hit
ATGCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCCAGGGGTGACGGACAGAGAGAGTGGAGT15880.13165257978535988No Hit
CTCCAGAGAACCGCTTGGAAACTTCATAGATCAGGAGCTGTGGAGGCTGG15830.13123805654925985No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15700.13016029613539987No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15640.12966286825207987No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGCTCCAGTGCACATATTGTGATGACCCAGACTCCACT15640.12966286825207987No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15630.12957996360485988No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15620.12949705895763985No Hit
GTGTATTACTGTGCGAGAGGCGGGGAGCATGATAGTAGTGGTTATTACGGACGCATGACAACACTTGACTACTA15480.12833639389655987No Hit
GCTAATGCTCTGGATACCTGGCTCCAGTGCACATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCC15420.12783896601323985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15360.12734153812991988No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGCTCCAGTGCA15350.12725863348269986No Hit
GCCACAGTTCGTTCAATCTCCAGTCGTGTCCCTGGGCCGAAGGTGATAGG15170.12576634983273988No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14840.12303049647447988No Hit
ATCTCCAGTCGTGTCCCTGGGCCGAAGGTGATAGGAAGCTGTATACTTTGCATGCAGTAATAAACCCCAACATC14710.12195273606061988No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14560.12070916635231989No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14500.12021173846899988No Hit
CCTGCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAG14340.1188852641134799No Hit
GGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAAGTATACAGC14260.11822202693571987No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGCTCCAGTGCACATATTGTGATGACCCAGACTC14210.11780750369961988No Hit
GTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAAAATCAGCCGGG14120.11706136187463988No Hit
AGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTC14080.11672974328575988No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14000.11606650610799989No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14000.11606650610799989No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13890.11515455498857989No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13840.11474003175247988No Hit
ATCTGGCACTCCAGAGAACCGCTTGGAAACTTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGCAGGTA13630.11299903416085988No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13430.1113409412164599No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13360.1107606086859199No Hit
CCTTCGGCCCAGGGACACGACTGGAGATTGAACGAACTGTGGCTGCACCA13240.10976575291927988No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13070.10835637391653989No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13040.10810765997487988No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12890.10686409026657989No Hit
CTCCAGTCGTGTCCCTGGGCCGAAGGTGATAGGAAGCTGTATACTTTGCA12840.1064495670304799No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12800.10611794844159989No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12760.1057863298527199No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12740.1056205205582799No Hit
GCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCC12680.1051230926749599No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12620.10462566479163989No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12390.1027188579055799No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT12370.1025530486111399No Hit
GATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGCAGGTACCAATACAAAT12350.10238723931669991No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12280.1018069067861599No Hit
GGACACGACTGGAGATTGAACGAACTGTGGCTGCACCATCTGTCTTCATC12170.1008949556667399No Hit
GTCGTGTCCCTGGGCCGAAGGTGATAGGAAGCTGTATACTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCT12160.1008120510195199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)