FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782952
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30230.3861028517712452No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATATCCTAGA29260.3737138419724325No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC26780.34203884784763305No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25380.32415780277718176No Hit
CCCCTGTACTTGTCATCTATGGTAATTACAACCGGCCCTCAGGGATCCCA24180.3088311927167949No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTT23280.29733623517150476No Hit
AGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAATTACAACCGGCCC22700.28992837364231777No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20720.2646394670426795No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC20560.2625959190346279No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18510.23641296018146707No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18420.23526346442693807No Hit
ATGTATTACTGTGCGAGAGAGAATCCCCTAACTGGCTTCGACCCCTGGGGCCAGGGAACCCTGGTCACTGTCTC18210.23258130766637036No Hit
GTAATACATAGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTGCAGATACA17520.2237685068816479No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17480.223257619879635No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAC16680.2130398798393771No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16040.2048656878071708No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATATCCTAGAT14860.1897945212477904No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT14780.1887727472437646No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14600.1864737557347066No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14360.18340843372262924No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14230.18174805096608732No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13740.17548968519142935No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA13300.1698699281692875No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13250.1692313194167714No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG12670.16182345788758443No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA12460.15914130112701672No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12440.1588858576260103No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA12350.15773636187148127No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT12230.15620370086544258No Hit
CTATTACTGTAACTCCCGGGACATCAATAATAACCATCTAGGATATGTGGTCTTCGGCGGAGGGACCAAGCTGAC12210.15594825736443613No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12190.1556928138634297No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11520.1471354565797137No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11510.14700773482921048No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11490.14675229132820403No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11340.14483646507065567No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11250.14368696931612665No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11190.14292063881310732No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11130.142154308310088No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11120.14202658655958475No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10460.133596951026372No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAACCTATTATGCAAGCTGGTA10110.12912668975875916No Hit
GACTATTACTGTAACTCCCGGGACATCAATAATAACCATCTAGGATATGT9820.12542275899416566No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9760.12465642849114633No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9690.12376237623762376No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTGCAGATA9690.12376237623762376No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9590.12248515873259153No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9390.11993072372252706No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9340.11929211497001092No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9100.11622679295793356No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8980.11469413195189489No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8970.11456641020139166No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8820.1126505839438433No Hit
GGACAGGCCCCTGTACTTGTCATCTATGGTAATTACAACCGGCCCTCAGG8790.11226741869233363No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8720.11137336643881107No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8680.11086247943679817No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC8610.1099684271832756No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8410.10741399217321113No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8330.10639221816918533No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8220.10498727891364988No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG8220.10498727891364988No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT8180.10447639191163698No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG8170.10434867016113375No Hit
CCACATATCCTAGATGGTTATTATTGATGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCC8110.10358233965811442No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAATTACAACCGGCC8100.1034546179076112No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8040.10268828740459185No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8020.1024328439035854No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7970.10179423515106929No Hit
ACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAACCTATTATG7950.10153879165006284No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7900.10090018289754674No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7900.10090018289754674No Hit
GTATTACTGTGCGAGAGAGAATCCCCTAACTGGCTTCGACCCCTGGGGCC7890.10077246114704351No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT401.1546377E-464.8717670
ACCGAAC200.007776602651.0310416
GGTATCA5100.042.687181
TCTCGTA856.91216E-1140.4141138
CGTATGC955.456968E-1239.96413841
TCGTATG955.456968E-1239.90983240
GTACGTA655.0595663E-639.7709369
GTGGTAT1300.039.2496341
GGACCTT350.001423200838.99286330
GTATCAA12600.035.6361731
ATCTCGT1004.0199666E-1034.32100737
CACGAGA1103.274181E-1134.0185221
TCCATAC400.002781882634.016353
CTTATAC1103.274181E-1134.016351
TATGCCG1154.5474735E-1133.114743
TATACAC1155.638867E-1132.5373763
CAAACGA552.9995388E-431.80546649
TATCAAC14350.031.29032
TATCTCG1101.1332304E-931.16276636
CCGTCTT1251.10958354E-1030.66683447
Produced by FastQC (version 0.11.5)