FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782952
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27040.3453596133607169No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATATCCTAGA25950.3314379425558655No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC24520.3131737322339045No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23400.2988688961775435No Hit
CCCCTGTACTTGTCATCTATGGTAATTACAACCGGCCCTCAGGGATCCCA23350.29823028742502733No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTT22200.2835422861171566No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC20920.267193902052744No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20530.26221275378311826No Hit
AGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAATTACAACCGGCCC20040.25595438800846027No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19610.2504623527368217No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17570.22440711563416404No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17230.22006457611705443No Hit
ATGTATTACTGTGCGAGAGAGAATCCCCTAACTGGCTTCGACCCCTGGGGCCAGGGAACCCTGGTCACTGTCTC17130.21878735861202223No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15700.2005231482900612No Hit
GTAATACATAGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTGCAGATACA15460.19745782627798386No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAC15200.1941370607649No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15150.19349845201238391No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT14680.1874955297387324No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14420.18417476422564857No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14200.18136488571457765No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13990.17868272895400994No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA13860.17702234619746807No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13360.17063625867230686No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13060.16680460615721016No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATATCCTAGAT12560.16041851863204895No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA12480.15939674462802317No Hit
CTATTACTGTAACTCCCGGGACATCAATAATAACCATCTAGGATATGTGGTCTTCGGCGGAGGGACCAAGCTGAC12430.15875813587550705No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12400.15837497062399739No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA12360.1578640836219845No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12350.15773636187148127No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG12170.15543737036242325No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT11980.153010657102862No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11710.14956216983927495No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11540.14739090008072014No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11400.145602795573675No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11390.1454750738231718No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11170.14266519531210087No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10940.13972759505052673No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10760.13742860354146869No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAACCTATTATGCAAGCTGGTA10390.1327028987728494No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10150.12963757676077203No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10040.12823263750523659No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9720.12414554148913344No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9680.12363465448712053No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9350.11941983672051415No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9290.11865350621749482No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8990.1148218537023981No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8950.11431096670038521No Hit
GACTATTACTGTAACTCCCGGGACATCAATAATAACCATCTAGGATATGT8900.11367235794786908No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8780.1121396969418304No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8640.11035159243478529No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8370.10690310517119823No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8350.10664766167019178No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC8290.10588133116717245No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG8250.10537044416515955No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8190.1046041136621402No Hit
ACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAACCTATTATG8130.10383778315912087No Hit
CCACATATCCTAGATGGTTATTATTGATGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCC8090.10332689615710797No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7900.10090018289754674No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAATTACAACCGGCC7890.10077246114704351No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7840.10013385239452738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCT200.00763387851.27068333
GTTACCG306.4342323E-445.81859638
GGTATCA5400.045.2508051
TTACCGC350.001361350639.34688639
GTATCAA13550.037.0689241
AATGGTA4050.034.84120670
TATCAAC15550.032.074772
TCAACGC15800.031.5692774
GTGGTAT1400.031.5139521
ATCAACG15850.031.4696853
ATCTTTA450.00418666831.29257851
CAACGCA15950.031.2723835
AACGCAG16150.030.891036
TACTATT450.004711961430.5457338
CGACCGG2800.030.32512311
TCGTCGA450.004916656830.28132630
CCGACCG2850.029.79119910
ACGGGAT803.1182753E-629.7186216
GTCGCTG3000.029.4130211
CGCAGAG17300.028.6542158
Produced by FastQC (version 0.11.5)