FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210113
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGTACAGCCACTGTAGTCACCTTTAGCGGCTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG26870.22204537923317902No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25810.21328586669178828No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24680.20394789577502268No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23170.1914697222490792No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22950.1896517102121868No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22750.18799897199683005No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21840.18047901311695683No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21670.17907418563390362No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21340.17634716757856497No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20630.17047994691404852No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20450.16899248252022744No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20280.1675876550371742No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19460.16081142835421156No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18470.15263037418819564No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18290.15114290979437456No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC18020.14891171320364296No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT17120.14147439123453762No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17050.14089593285916274No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17010.14056538521609138No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16480.136185628945396No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16100.1330454263362182No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15890.13131005121009362No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15810.1306489559239509No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14980.1237900923302204No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14700.12147625882872096No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14640.12098043736411394No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14620.12081516354257825No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14530.12007143134566771No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14520.11998879443489989No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14520.11998879443489989No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14380.11883187768415016No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14290.11808814548723962No Hit
AGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT14240.11767496093340044No Hit
GTGTGGGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTGCAGCCGGGGGGGTCCCTGAGACTCTCCTGTACAGC14200.11734441329032909No Hit
GAAATATATGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA14170.11709650255802558No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14090.11643540727188288No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG14060.11618749653957937No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13800.1140389368596156No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13620.11255147246579451No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG13520.11172510335811614No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC13500.11155982953658047No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG13330.11015500205352723No Hit
ACCAAATACTACGCAGACTCCGTGAGGGGCCGGTTCACCGTCTCCAGAGACAATTCCAAGAACACACTGTATCTG13320.1100723651427594No Hit
CACTTGCACTGCTGCCTACCCCGAGTCCAAGACCCCGCTAACCGCCACCC13180.10891544839200966No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13140.10858490074893833No Hit
ATATATTTCTGTGCGATAGAGCATCTGTTTTCAGGGGGATGGGGACCACC13120.10841962692740265No Hit
AGGTAGTATGGGGTCGGTGGTCCCCATCCCCCTGAAAACAGATGCTCTAT13120.10841962692740265No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA13110.10833699001663481No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13080.10808907928433131No Hit
ATACTACCTTGACTACTGGGGCCTGGGAACCCAGGTCACCGTCTCCTCCGCATCCCCGACCAGCCCCAAGGTCTT12800.10577524578283186No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12760.1054446981397605No Hit
GTATCAACAGAGACCAGGAAAAGCCCCTAAACTCCTAGTCCATAAAAACT12710.10503151358592132No Hit
GCCCCAAGGACGTGCTGGTTCGCTGGCTGCAGGGGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCAT12470.10304822772749322No Hit
AGTCAGGACATTGGAGATAATTTAGGCTGGTATCAACAGAGACCAGGAAA12420.10263504317365403No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12170.10056912040445809No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTCG700.001572793938.20649370
AATGGTA4400.036.46983370
GTGGTAT3550.036.3441431
GGGTACT751.8777118E-631.71838819
ATCAACG36900.031.0865173
TCAACGC37600.030.6882974
CAACGCA38400.030.0489585
AACGCAG38500.029.9783426
GTATCAA40900.029.8023361
CGCAGAG40100.028.8859548
CTAAGCT605.969582E-428.2813724
AGAGTAC40550.028.23966211
CAGAGTA40850.028.03110910
GCAGAGT41750.027.5850549
CGGGTAC1254.8894435E-927.1804418
CTAGGTC756.89357E-527.1501184
TATCAAC45150.026.8366372
TATATGC801.0602439E-425.48482513
AGTACTT15550.025.12973213
GAGTACT15750.025.02637112
Produced by FastQC (version 0.11.5)