FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259772
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACCATGTCTCCTGGTACCAGCAGTTCCCAGGCAAAGCCCCCAAAC47230.37490911053746234No Hit
GTCCATGGTGGGTCCGTCAGTGGTTACTACTGGAGTTGGATCCGCCAGCC43640.34641189040556547No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGAGTGTAG39100.3103736231635566No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG36000.28576599575161216No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33000.2619521627723112No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC31920.2533791828997628No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG31490.24996586683939637No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGAGTGTAGGTGCCTCTATAGGAG29530.23440749595958635No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29040.23051790323963384No Hit
GTGTCCGGGTCTCCTGGGCAGTCAGTCACCATCTCCTGCACTGGAACCAG28470.22599327497356664No Hit
TGGTTATACCCACCAATATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGCCCAGGAGACCCGGACA28360.22512010109765895No Hit
CCCAAAGAGTGTAGGTGCCTCTATAGGAGCAGCAGTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAGAGATGG28140.22337375334584353No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27910.22154802615076377No Hit
CCTATAGAGGCACCTACACTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTG27280.21654712122511055No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27240.21622960345205325No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27240.21622960345205325No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC26230.2082122796823552No Hit
CCTGAGGGCCGCTTAGTGACATCATAAATCATGAGTTTGGGGGCTTTGCC25370.20138564756162228No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT25220.2001949559126572No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25070.19900426426369217No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCTTGGG24370.1934477032351886No Hit
GATTATTACTGCTGCTCCTATAGAGGCACCTACACTCTTTGGGTGTTCGG23790.18884369552585706No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23310.1850334822491689No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGTGGGTATAACCATGTCTCCTGGTACCAGCAGTTCCCAGGCAAAGC23000.18257271950797446No Hit
CTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCC22960.18225520173491713No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22470.17836560901496462No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22420.17796871179864293No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22170.1759842257170345No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21950.1742378779652191No Hit
GCCCTCAGGTGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG21580.17130083856443865No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21570.1712214591211743No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21500.17066580301832396No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21240.1686019374934512No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20900.16590303642246376No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20450.16233096147556858No Hit
GTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAGAGATGGCCAGGGAGG19650.15598060601442165No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18580.14748700558513764No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18430.14629631393617257No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18360.14574065783332224No Hit
ATAATATACAGCCGTGTCCGCGGCGTTCACAGAGGTCAGCCTCAGGGAGA18330.14550251950352922No Hit
GTCTGGAGCCCAGAGATGGCCAGGGAGGCCGTGTTGCCAGACTTGGAGCC18190.1443912072978285No Hit
TCTCTGGGCTCCAGACTGAGGATGAGGCTGATTATTACTGCTGCTCCTAT17950.14248610065948442No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCTTGGGCTCAGTCTGCCCTGACTCAGC17690.14042223513461166No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17490.13883464626932493No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17340.1376439546203599No Hit
GTGTAGGTGCCTCTATAGGAGCAGCAGTAATAATCAGCCTCATCCTCAGT17220.13669140130118784No Hit
CTCTATAGGAGCAGCAGTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAGAGATGGCCAGGGAGGCCGTGTTGC17110.13581822742528013No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17080.1355800890954871No Hit
CTATAGGAGCAGCAGTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAGA16750.13296056746776402No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16690.13248429080817797No Hit
ATCTTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCACCATCTCCTG16580.1316111169322703No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15990.12692772977967443No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCAC15770.125181382027859No Hit
ACCATGGACAGCGCACGTGAGGGACAGGGTCTCCGAAGGCTCCAACAGTCCTGCGCCCCACTGCTGGAGCTGCAC15730.12486386425480166No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15590.12375255204910093No Hit
CACCTACACTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGT15510.12311751650298626No Hit
GGGCACAGGATCTTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCAC15300.12145054819443518No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC15240.12097427153484916No Hit
TCCTCACTCAGGGCACAGGATCTTGGGCTCAGTCTGCCCTGACTCAGCCT15010.11914854433976942No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT14980.1189104060099764No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT14710.11676716104183932No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14680.1165290227120463No Hit
CTCCTGGGCAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATT14540.1154177105063456No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14520.11525895161981692No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14500.11510019273328824No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14480.11494143384675959No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCACCATCT14380.1141476394141162No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14220.11287756832188682No Hit
GGGTATAACCATGTCTCCTGGTACCAGCAGTTCCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCACTAAG14080.11176625611618611No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC13900.11033742613742803No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCACCATCTCCTGCA13860.1100199083643707No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13860.1100199083643707No Hit
GCTCCAGACTGAGGATGAGGCTGATTATTACTGCTGCTCCTATAGAGGCA13820.10970239059131334No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG13770.10930549337499165No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13690.10867045782887697No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTC13510.10724162785011893No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13420.10652721286073988No Hit
CCTACACTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCA13370.1061303156444182No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCTTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT13260.1052571417685105No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCTTGGGCTCAGTCTGCCCTGACT13100.1039870706762811No Hit
GTCATGGACCTCCTGCACAAGAGCATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC12940.10271699958405171No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12820.10176444626487967No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC12610.1000974779563286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT1050.052.3708337
CGTATGC1200.051.83069241
TGCCGTC1250.050.0294245
TCGTATG1300.047.77062640
ATGCCGT1350.046.27482244
AGGGGGG1601.8189894E-1245.6388770
ACATGCG1350.045.49287427
ACGAGAC1350.045.4224422
GGTATCA8200.045.278571
GATCTCG1350.043.22500636
ATGCGCA1450.042.39083529
TGGATTG15700.041.8598570
CTTATAC1800.039.7399061
CATGCGC1550.039.63543728
GTGGTAT2200.038.70771
TATACAC1850.038.665853
GGATGGG12200.038.0890770
CCGTCTT1650.038.02114547
AGATCTC1550.037.6250135
TGCGCAG1650.037.2792530
Produced by FastQC (version 0.11.5)