FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259772
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACCATGTCTCCTGGTACCAGCAGTTCCCAGGCAAAGCCCCCAAAC44400.35244472809365507No Hit
GTCCATGGTGGGTCCGTCAGTGGTTACTACTGGAGTTGGATCCGCCAGCC40130.31854970581978326No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG38500.3056108565676964No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGAGTGTAG35540.2821145413614527No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33920.2692550715526302No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC30110.23901150366891785No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA29990.23805895034974583No Hit
GTGTCCGGGTCTCCTGGGCAGTCAGTCACCATCTCCTGCACTGGAACCAG29150.23139107711554152No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28440.22575513664377364No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGAGTGTAGGTGCCTCTATAGGAG27880.22130988782097077No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27660.21956354006915538No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27040.21464201458676646No Hit
TGGTTATACCCACCAATATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGCCCAGGAGACCCGGACA27000.21432449681370916No Hit
CCTATAGAGGCACCTACACTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTG25780.20464020473546007No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25690.20392578974608105No Hit
CCCAAAGAGTGTAGGTGCCTCTATAGGAGCAGCAGTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAGAGATGG25170.19979805869633555No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24580.19511467154373965No Hit
CTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCC24100.1913044582670515No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24000.19051066383440812No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC23560.18701796833077733No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCTTGGG23280.18479534391937588No Hit
CCTGAGGGCCGCTTAGTGACATCATAAATCATGAGTTTGGGGGCTTTGCC22760.18066761286963037No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22070.17519043128439116No Hit
GATTATTACTGCTGCTCCTATAGAGGCACCTACACTCTTTGGGTGTTCGG22060.1751110518411268No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21960.17431725740848344No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGTGGGTATAACCATGTCTCCTGGTACCAGCAGTTCCCAGGCAAAGC21580.17130083856443865No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21260.16876069637997987No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20990.16661745141184278No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20580.16336289423800496No Hit
GCCCTCAGGTGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG20420.16209282314577558No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20400.16193406425924692No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20060.15923516318825945No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19670.15613936490095034No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19430.15423425826260626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19410.1540754993760776No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19190.15232915162426217No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19090.15153535719161879No Hit
GTCTGGAGCCCAGAGATGGCCAGGGAGGCCGTGTTGCCAGACTTGGAGCC18900.15002714776959641No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18770.14899521500716No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC18520.14701072892555161No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18490.1467725905957586No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18010.14296237731907044No Hit
GTGTAGGTGCCTCTATAGGAGCAGCAGTAATAATCAGCCTCATCCTCAGT17970.14264485954601308No Hit
GTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAGAGATGGCCAGGGAGG17640.14002533791828997No Hit
TCTCTGGGCTCCAGACTGAGGATGAGGCTGATTATTACTGCTGCTCCTAT17610.13978719958849697No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCTTGGGCTCAGTCTGCCCTGACTCAGC17370.13788209295015288No Hit
ATAATATACAGCCGTGTCCGCGGCGTTCACAGAGGTCAGCCTCAGGGAGA17150.1361357451983375No Hit
GGGCACAGGATCTTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCAC16710.13264304969470667No Hit
CTCTATAGGAGCAGCAGTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAGAGATGGCCAGGGAGGCCGTGTTGC16400.13018228695351222No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16130.12803904198537513No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16110.12788028309884647No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCAC16000.12700710922293876No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15800.12541952035765203No Hit
CTATAGGAGCAGCAGTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAGA15430.12248248095687157No Hit
ATCTTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCACCATCTCCTG15390.12216496318381422No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15180.12049799487526315No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT15160.12033923598873447No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15160.12033923598873447No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15010.11914854433976942No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCACCATCT14770.11724343770142534No Hit
GGGTATAACCATGTCTCCTGGTACCAGCAGTTCCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCACTAAG14750.11708467881489666No Hit
TCCTCACTCAGGGCACAGGATCTTGGGCTCAGTCTGCCCTGACTCAGCCT14650.1162908843822533No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14500.11510019273328824No Hit
ACCATGGACAGCGCACGTGAGGGACAGGGTCTCCGAAGGCTCCAACAGTCCTGCGCCCCACTGCTGGAGCTGCAC14420.11446515718717355No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14310.11359198331126585No Hit
CTCCTGGGCAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATT14230.11295694776515117No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13920.11049618502395671No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13870.11009928780763505No Hit
CACCTACACTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGT13860.1100199083643707No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13760.10922611393172732No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13440.10668597174726856No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC13380.10620969508768255No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13360.10605093620115387No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13360.10605093620115387No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCACCATCTCCTGCA13340.10589217731462519No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13160.10446334733586711No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC12930.10263762014078737No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12930.10263762014078737No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12880.1022407229244657No Hit
CCTACACTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCA12860.10208196403793703No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTC12810.10168506682161534No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCTTGGGCTCAGTCTGCCCTGACT12730.10105003127550065No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC12670.10057375461591461No Hit
GTCATGGACCTCCTGCACAAGAGCATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC12660.1004943751726503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)