FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144306
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49600.43345049313732514No Hit
GTGTTAGTTGTTGTATATAAGCTGCAGTAATAATCAGCCTCGTCCTCAGG46680.4079328431381117No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAATAGCGCAGTGTTAGTTGTTGTATATAAGCTGCAGTAATAAT44470.38861982721404936No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAATAGCGCAGTGTTAGTTGTTGTATATAAGCTG43660.38154130101563744No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG43590.3809295765293549No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG41010.3583831597492279No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTT37380.3266608756748632No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35610.3111929850931482No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34460.30114322567564966No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31730.2772859707106316No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31020.27108133663548034No Hit
GTAATAAACAGCCGTGTCCTCCTCTCTCAGGCTATTCATTTGCAGATATA30960.27055700136152394No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTC30290.264701924135677No Hit
CTCCTGCACTGGAACCAACAGTGACGTTGGTGACTATAATTATGTCTCCT29250.25561344605376535No Hit
CTATAATTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGGTCCCAGAC27530.2405825015336807No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTACTGCAGCTTATAT27100.23682476540365952No Hit
GTTTATTACTGTGCGAGAGACCTTAGTTTCGGATCTTGGGAGTTCTGGGGCCAGGGAACCCTGGTCGCCGTCTC25630.22397855119172672No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25470.2225803237945095No Hit
GTCCCAGACTCATGATTTTTGAGGTCAGTAAGCGGCCCTCAGGGATTTCT25040.21882258766448837No Hit
CAACTAACACTGCGCTATTCGGCGGAGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA24450.21366662413725No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24060.21025844485653314No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTTGGTGACTATAATTATGTCTCCTGGTACCAACAGCA23350.20405381078138193No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23160.20239341574718653No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22660.19802395513088283No Hit
GATTATTACTGCAGCTTATATACAACAACTAACACTGCGCTATTCGGCGGAGGGACCACGCTGACCGTCCTAGGT22570.19723745221994815No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22570.19723745221994815No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22500.19662572773366566No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC22270.19461577585016596No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21300.1861390222545368No Hit
ACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAATAGCGCAGTGTTAGTTG20710.18098305872729847No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20480.17897310684379877No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20280.1772253225972773No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20190.17643881968634265No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGAAGG19760.17268108355632147No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19410.1696224611249089No Hit
GTCTGGGACCTTTGCCTGGGTGCTGTTGGTACCAGGAGACATAATTATAG19220.16796206609071349No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT19080.16673861711814847No Hit
TAATTATAGTCACCAACGTCACTGTTGGTTCCAGTGCAGGAGATGGTGAT19050.16647644948117024No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18860.16481605444697484No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18810.16437910838534447No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18600.16254393492649694No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17990.15721319297460645No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17840.15590235478971534No Hit
GCCCAGAGATGGTCAGGAAGGCCGTGCTGCCAGACTTGGAGCCAGAGAAG17640.15415457054319387No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17440.1524067862966724No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17110.14952294228991198No Hit
AACTAACACTGCGCTATTCGGCGGAGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA17070.14917338544060768No Hit
GGTACCAACAGCACCCAGGCAAAGGTCCCAGACTCATGATTTTTGAGGTC16840.14716343355710798No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG15690.1371136741396095No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC15430.13484155461913158No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15380.13440460855750125No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15160.13248204588632762No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14960.13073426163980614No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14840.12968559109189326No Hit
CTCCTGTGCAGTGACTGGATTCACCCTCAGTAGCCATGGCATGCTCTGGGTCCGCCAGGCTCCAGGCAACGGT14830.1295982018795672No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14750.1288990881809586No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT14730.12872430975630644No Hit
GTACCAACAGCACCCAGGCAAAGGTCCCAGACTCATGATTTTTGAGGTCA14660.12811258527002392No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG14420.12601524417419815No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14410.1259278549618721No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14400.125840465749546No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14120.12339356780441595No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14030.12260706489348129No Hit
GTATATAAGCTGCAGTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGA13900.12147100513324233No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13880.1212962267085902No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13830.12085928064695982No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13830.12085928064695982No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13810.12068450222230767No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13650.11928627482509048No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13640.11919888561276443No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13550.11841238270182976No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC13460.11762587979089509No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13280.11605287396902576No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13020.11378075444854786No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12990.11351858681156962No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12920.11290686232528711No Hit
GGAACCAACAGTGACGTTGGTGACTATAATTATGTCTCCTGGTACCAACA12830.11212035941435246No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12770.11159602414039602No Hit
CAACAGCACCCAGGCAAAGGTCCCAGACTCATGATTTTTGAGGTCAGTAAGCGGCCCTCAGGGATTTCTAATCG12690.11089691044178743No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12610.11019779674317884No Hit
GCTTATATACAACAACTAACACTGCGCTATTCGGCGGAGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGGCTG12590.11002301831852669No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12590.11002301831852669No Hit
ATATACAACAACTAACACTGCGCTATTCGGCGGAGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC12570.10984823989387453No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12190.10652744982548375No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12170.1063526714008316No Hit
CTCCAGACCGTTGCCTGGAGCCTGGCGGACCCAGAGCATGCCATGGCTAC11980.1046922763666362No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11980.1046922763666362No Hit
GGCTGGAGCCCAGAGATGGTCAGGAAGGCCGTGCTGCCAGACTTGGAGCC11900.1039931626680276No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11870.10373099503104939No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11780.10294449212011472No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11570.10110931866126717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG501.6370905E-1162.1530140
CGTATGC652.1100277E-1047.85011341
TCTCGTA605.678885E-945.9379338
GGTATCA8000.044.8881871
TATCTCG702.2482709E-839.30961636
AGACCGG801.7807906E-938.49072625
TCGGCAA559.795168E-538.47752469
GTATCAA19950.037.200571
GCCTATC754.1965905E-836.6213833
GAGACCG853.2232492E-936.223424
AATGGTA5350.035.9082470
GTGGTAT2050.035.0346831
ATGCCGT904.953108E-934.66555844
ATCTCGT807.2403054E-834.42011637
GACCGGA905.6224962E-934.21846826
TCTCCGA1004.1836756E-1034.2020211
CGTCGGA1557.66795E-834.1907270
CGTCTTC958.021743E-932.99449548
TATCAAC22550.032.7597052
TATACAC1400.031.7576313
Produced by FastQC (version 0.11.5)