FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144306
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAGTTGTTGTATATAAGCTGCAGTAATAATCAGCCTCGTCCTCAGG48590.4246241826923917No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46130.40312643646017765No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAATAGCGCAGTGTTAGTTGTTGTATATAAGCTGCAGTAATAAT42520.37157893081046506No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAATAGCGCAGTGTTAGTTGTTGTATATAAGCTG42190.3686950868037046No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG41310.36100483611901013No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37980.3319042284144276No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37620.32875821677068895No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTT36760.32124274451064666No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33210.29021957413489047No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30760.2688092171150025No Hit
CTCCTGCACTGGAACCAACAGTGACGTTGGTGACTATAATTATGTCTCCT29530.2580603439988954No Hit
GTAATAAACAGCCGTGTCCTCCTCTCTCAGGCTATTCATTTGCAGATATA29160.25482694314283066No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTC28330.24757363851976655No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28270.24704930324581012No Hit
GTCCCAGACTCATGATTTTTGAGGTCAGTAAGCGGCCCTCAGGGATTTCT25880.22616328149987855No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25420.22214337773287912No Hit
CTATAATTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGGTCCCAGAC25410.22205598852055308No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTACTGCAGCTTATAT24800.21672524656866257No Hit
GTTTATTACTGTGCGAGAGACCTTAGTTTCGGATCTTGGGAGTTCTGGGGCCAGGGAACCCTGGTCGCCGTCTC24780.21655046814401044No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24090.21052061249351137No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC23730.2073746008497727No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23400.20449075684301227No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23320.20379164314440365No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22990.20090779913764326No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTTGGTGACTATAATTATGTCTCCTGGTACCAACAGCA22720.1985482904048393No Hit
CAACTAACACTGCGCTATTCGGCGGAGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA22640.19784917670623067No Hit
GATTATTACTGCAGCTTATATACAACAACTAACACTGCGCTATTCGGCGGAGGGACCACGCTGACCGTCCTAGGT21490.1877994172887322No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21170.18500296249429785No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20660.18054611266566808No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20650.18045872345334202No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19680.17198196985771289No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19050.16647644948117024No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19030.16630167105651808No Hit
GTCTGGGACCTTTGCCTGGGTGCTGTTGGTACCAGGAGACATAATTATAG19020.166214281844192No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGAAGG18250.15948531249508435No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18130.15843664194717147No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC18020.15747536061158468No Hit
ACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAATAGCGCAGTGTTAGTTG17950.15686363612530213No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17640.15415457054319387No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT17510.1530185107829549No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17370.15179506181038988No Hit
GCCCAGAGATGGTCAGGAAGGCCGTGCTGCCAGACTTGGAGCCAGAGAAG17130.1496977207145641No Hit
TAATTATAGTCACCAACGTCACTGTTGGTTCCAGTGCAGGAGATGGTGAT17050.14899860701595552No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16620.14524087088593435No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16580.14489131403663005No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16450.1437552542763911No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16220.1417453023928914No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15830.13833712311217455No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG15760.137725398625892No Hit
CTCCTGTGCAGTGACTGGATTCACCCTCAGTAGCCATGGCATGCTCTGGGTCCGCCAGGCTCCAGGCAACGGT15330.13396766249587086No Hit
GGTACCAACAGCACCCAGGCAAAGGTCCCAGACTCATGATTTTTGAGGTC15230.1330937703726101No Hit
AACTAACACTGCGCTATTCGGCGGAGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA15180.13265682431097975No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14910.13029731557817578No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14910.13029731557817578No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT14610.12767563920839356No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14430.12610263338652422No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14410.1259278549618721No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14150.12365573544139416No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14120.12339356780441595No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14110.12330617859208988No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13980.12217011883185092No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13810.12068450222230767No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13800.1205971130099816No Hit
GTACCAACAGCACCCAGGCAAAGGTCCCAGACTCATGATTTTTGAGGTCA13680.11954844246206871No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13570.11858716112648189No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13560.11849977191415582No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13100.11447986814715644No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12970.11334380838691749No Hit
GGCTGGAGCCCAGAGATGGTCAGGAAGGCCGTGCTGCCAGACTTGGAGCC12910.11281947311296105No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12890.1126446946883089No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG12810.11194558098970031No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12590.11002301831852669No Hit
GTATATAAGCTGCAGTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGA12500.10923651540759204No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12480.10906173698293987No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12400.10836262328433129No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12330.10775089879804878No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12310.10757612037339663No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12220.10678961746246197No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12130.10600311455152729No Hit
ATATACAACAACTAACACTGCGCTATTCGGCGGAGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC12000.10486705479128836No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11900.1039931626680276No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11720.1024201568461583No Hit
GCTTATATACAACAACTAACACTGCGCTATTCGGCGGAGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGGCTG11680.10207059999685399No Hit
CAACAGCACCCAGGCAAAGGTCCCAGACTCATGATTTTTGAGGTCAGTAAGCGGCCCTCAGGGATTTCTAATCG11640.1017210431475497No Hit
GGAACCAACAGTGACGTTGGTGACTATAATTATGTCTCCTGGTACCAACA11590.10128409708591933No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11590.10128409708591933No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTC11570.10110931866126717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAA100.0088107195134.8348570
TACGTAT409.423002E-784.2717970
GTGGTAT2050.049.974451
GGTATCA8050.043.2698021
GTATCAA20100.038.906311
GTACGTA559.578337E-538.62402769
CGAACTA754.3102773E-836.51038729
TATCAAC22050.035.614262
TGGTATC2950.034.721942
GGTACGT551.8383414E-434.57288768
ATACGTA807.707604E-834.1760911
TCCTATA501.9786414E-434.1432382
TATACAG501.9786414E-434.1432382
ATCAACG23150.033.7730453
TCAACGC23450.033.340984
CAACGCA24450.031.8390925
AACGCAG24750.031.4586646
AATGGTA4100.031.24222470
AGTCGCG450.004706788330.55381435
GCGAACT902.1448068E-730.41466928
Produced by FastQC (version 0.11.5)